Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis

The aim of this work was to study the oxidative stress effects and histological alterations caused by dietary copper on the filter-feeding freshwater mussel Diplodon chilensis. Bivalves were fed during 6 weeks with the green algae Scenedesmus vacuolatus previously exposed to copper. Metal concentrat...

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Autores principales:	Sabatini, Sebastián Eduardo, Rocchetta, Iara, Nahabedian, Daniel Eduardo, Luquet, Carlos Marcelo, Ríos de Molina, María del Carmen
Publicado:	2011
Materias:	Antioxidant defenses Dietary copper Diplodon chilensis Metal bioaccumulation Oxidative stress copper fresh water rhodanine article bivalve Chlorophyceae controlled study dietary intake exocrine gland freshwater species histology nonhuman oxidative stress priority journal Scenedesmus Animals Bivalvia Catalase Copper Fresh Water Glutathione Lipid Peroxidation Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances algae Chlorophyta Scenedesmus vacuolatus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v154_n4_p391_Sabatini http://hdl.handle.net/20.500.12110/paper_15320456_v154_n4_p391_Sabatini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15320456_v154_n4_p391_Sabatini
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spelling	paper:paper_15320456_v154_n4_p391_Sabatini2023-06-08T16:19:55Z Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis Sabatini, Sebastián Eduardo Rocchetta, Iara Nahabedian, Daniel Eduardo Luquet, Carlos Marcelo Ríos de Molina, María del Carmen Antioxidant defenses Dietary copper Diplodon chilensis Metal bioaccumulation Oxidative stress copper fresh water rhodanine article bivalve Chlorophyceae controlled study dietary intake exocrine gland freshwater species histology nonhuman oxidative stress priority journal Scenedesmus Animals Bivalvia Catalase Copper Fresh Water Glutathione Lipid Peroxidation Oxidative Stress Scenedesmus Superoxide Dismutase Thiobarbituric Acid Reactive Substances algae Bivalvia Chlorophyta Diplodon chilensis Scenedesmus vacuolatus The aim of this work was to study the oxidative stress effects and histological alterations caused by dietary copper on the filter-feeding freshwater mussel Diplodon chilensis. Bivalves were fed during 6 weeks with the green algae Scenedesmus vacuolatus previously exposed to copper. Metal concentration in algae cultures and bivalve digestive gland was measured by TXRF. A maximum accumulation of 0.49 μg Cu/mg protein was detected at week 6. Also at this week, the hepatosomatic index (HSI) showed the highest decrease (50%) in response to Cu exposure. SOD and GST activities were significantly increased at weeks 4, 5 and 6, reaching an activity on average 50% higher than in controls for GST. CAT activity and GSH increased significantly at weeks 5 and 6. Despite this response, oxidative damage measured as TBARS and carbonyl groups contents increased significantly at weeks 4, 5 and 6, respectively. Digestive tubule and duct atrophy and cell-type replacement in treated mussels were observed by histological studies. The presence of intracellular rhodanine-positive granules, suggests copper accumulation in intracellular vacuoles of digestive cells. © 2011 Elsevier Inc. All rights reserved. Fil:Sabatini, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nahabedian, D.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos De Molina, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v154_n4_p391_Sabatini http://hdl.handle.net/20.500.12110/paper_15320456_v154_n4_p391_Sabatini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antioxidant defenses Dietary copper Diplodon chilensis Metal bioaccumulation Oxidative stress copper fresh water rhodanine article bivalve Chlorophyceae controlled study dietary intake exocrine gland freshwater species histology nonhuman oxidative stress priority journal Scenedesmus Animals Bivalvia Catalase Copper Fresh Water Glutathione Lipid Peroxidation Oxidative Stress Scenedesmus Superoxide Dismutase Thiobarbituric Acid Reactive Substances algae Bivalvia Chlorophyta Diplodon chilensis Scenedesmus vacuolatus
spellingShingle	Antioxidant defenses Dietary copper Diplodon chilensis Metal bioaccumulation Oxidative stress copper fresh water rhodanine article bivalve Chlorophyceae controlled study dietary intake exocrine gland freshwater species histology nonhuman oxidative stress priority journal Scenedesmus Animals Bivalvia Catalase Copper Fresh Water Glutathione Lipid Peroxidation Oxidative Stress Scenedesmus Superoxide Dismutase Thiobarbituric Acid Reactive Substances algae Bivalvia Chlorophyta Diplodon chilensis Scenedesmus vacuolatus Sabatini, Sebastián Eduardo Rocchetta, Iara Nahabedian, Daniel Eduardo Luquet, Carlos Marcelo Ríos de Molina, María del Carmen Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis
topic_facet	Antioxidant defenses Dietary copper Diplodon chilensis Metal bioaccumulation Oxidative stress copper fresh water rhodanine article bivalve Chlorophyceae controlled study dietary intake exocrine gland freshwater species histology nonhuman oxidative stress priority journal Scenedesmus Animals Bivalvia Catalase Copper Fresh Water Glutathione Lipid Peroxidation Oxidative Stress Scenedesmus Superoxide Dismutase Thiobarbituric Acid Reactive Substances algae Bivalvia Chlorophyta Diplodon chilensis Scenedesmus vacuolatus
description	The aim of this work was to study the oxidative stress effects and histological alterations caused by dietary copper on the filter-feeding freshwater mussel Diplodon chilensis. Bivalves were fed during 6 weeks with the green algae Scenedesmus vacuolatus previously exposed to copper. Metal concentration in algae cultures and bivalve digestive gland was measured by TXRF. A maximum accumulation of 0.49 μg Cu/mg protein was detected at week 6. Also at this week, the hepatosomatic index (HSI) showed the highest decrease (50%) in response to Cu exposure. SOD and GST activities were significantly increased at weeks 4, 5 and 6, reaching an activity on average 50% higher than in controls for GST. CAT activity and GSH increased significantly at weeks 5 and 6. Despite this response, oxidative damage measured as TBARS and carbonyl groups contents increased significantly at weeks 4, 5 and 6, respectively. Digestive tubule and duct atrophy and cell-type replacement in treated mussels were observed by histological studies. The presence of intracellular rhodanine-positive granules, suggests copper accumulation in intracellular vacuoles of digestive cells. © 2011 Elsevier Inc. All rights reserved.
author	Sabatini, Sebastián Eduardo Rocchetta, Iara Nahabedian, Daniel Eduardo Luquet, Carlos Marcelo Ríos de Molina, María del Carmen
author_facet	Sabatini, Sebastián Eduardo Rocchetta, Iara Nahabedian, Daniel Eduardo Luquet, Carlos Marcelo Ríos de Molina, María del Carmen
author_sort	Sabatini, Sebastián Eduardo
title	Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis
title_short	Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis
title_full	Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis
title_fullStr	Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis
title_full_unstemmed	Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis
title_sort	oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve diplodon chilensis
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v154_n4_p391_Sabatini http://hdl.handle.net/20.500.12110/paper_15320456_v154_n4_p391_Sabatini
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Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis

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