Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids

The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decre...

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Publicado:	2014
Materias:	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques Metal Nanoparticles Photons
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15306984_v14_n5_p2831_Pellegrotti http://hdl.handle.net/20.500.12110/paper_15306984_v14_n5_p2831_Pellegrotti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15306984_v14_n5_p2831_Pellegrotti
record_format	dspace
spelling	paper:paper_15306984_v14_n5_p2831_Pellegrotti2023-06-08T16:19:47Z Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles' size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching. © 2014 American Chemical Society. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15306984_v14_n5_p2831_Pellegrotti http://hdl.handle.net/20.500.12110/paper_15306984_v14_n5_p2831_Pellegrotti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons
spellingShingle	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
topic_facet	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons
description	The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles' size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching. © 2014 American Chemical Society.
title	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_short	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_full	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_fullStr	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_full_unstemmed	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_sort	controlled reduction of photobleaching in dna origami-gold nanoparticle hybrids
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15306984_v14_n5_p2831_Pellegrotti http://hdl.handle.net/20.500.12110/paper_15306984_v14_n5_p2831_Pellegrotti
_version_	1768544290044116992

Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids

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