Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase
Self-assembled multilayers comprised of alternate layers of polyphenol oxidase (PPO) and poly(allylamine) (PAH) or PPO and poly(diallyldimethylamine) (PDDA), deposited on a 3-mercaptopropanesulfonic acid (MPS)-modified gold surface, were studied "in-situ" (under water) by means of ellipsom...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15257797_v4_n4_p869_Forzani http://hdl.handle.net/20.500.12110/paper_15257797_v4_n4_p869_Forzani |
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paper:paper_15257797_v4_n4_p869_Forzani2023-06-08T16:19:36Z Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase Absorption spectroscopy Electric impedance Enzymes Fourier transform infrared spectroscopy Positive ions Self assembly Polycations Macromolecules 3 mercaptopropanesulfonic acid catechol oxidase enzyme gold poly(allylamine) poly(diallyldimethylamine) polycation polymer silicon dioxide sulfonic acid derivative thiol unclassified drug water absorption article calculation chemical structure comparative study controlled study density electricity ellipsometry enzyme structure enzyme substrate film gravimetry impedance infrared spectroscopy molecular model priority journal surface property thickness water content Catechol Oxidase Electrostatics Gold Microchemistry Polyamines Quartz Spectroscopy, Fourier Transform Infrared Sulfonic Acids Surface Properties Self-assembled multilayers comprised of alternate layers of polyphenol oxidase (PPO) and poly(allylamine) (PAH) or PPO and poly(diallyldimethylamine) (PDDA), deposited on a 3-mercaptopropanesulfonic acid (MPS)-modified gold surface, were studied "in-situ" (under water) by means of ellipsometry and quartz crystal microbalance (QCM), and "ex-situ" (in open air) by ellipsometry and fourier transform infrared reflection-absorption spectroscopy (FT-IRRAS). Optical ellipsometric properties of (PAH n(PPO)n and (PDDA)n(PPO)n multilayers were obtained at two wavelengths, employing an isotropic single-layer model with the substrate parameters measured after thiol adsorption. Film thickness as well as ellipsometric mass values based on the de Feijter equation were also evaluated. The quartz crystal impedance analysis showed that self-assembled multilayers behaved as acoustically thin films, and therefore, the shifts observed in the film inductive impedance parameter were interpreted in terms of gravimetric mass. The enzyme mass uptake in each adsorption step was determined on PAH or on PDDA topmost layer. A comparative study between the ellipsometric thickness and acoustic mass values allowed us to obtain average values of "apparent" densities of (2.1 ± 0.1) and (2.4 ± 0.1) g cm-3 for (PAH)n(PPO n and (PDDA)n(PPO)n multilayers, respectively. The content of water included in the open polymer-enzyme structure was evaluated by comparison of QCM and ellipsometric mass values. FT-IRRAS spectra of each layer in (PAH)n(PPO n and (PDDA)n(PPO)n films were recorded, and the intensity ratio of the amide bands was evaluated to obtain information about layer-by-layer enzyme conformation. An enzyme/polycation distribution model for (PAH)n-(PPO)n and (PDDA)n(PPO)n multilayer structures is presented on the basis of combined ellipsometric, QCM, and FT-IRRAS results. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15257797_v4_n4_p869_Forzani http://hdl.handle.net/20.500.12110/paper_15257797_v4_n4_p869_Forzani |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Absorption spectroscopy Electric impedance Enzymes Fourier transform infrared spectroscopy Positive ions Self assembly Polycations Macromolecules 3 mercaptopropanesulfonic acid catechol oxidase enzyme gold poly(allylamine) poly(diallyldimethylamine) polycation polymer silicon dioxide sulfonic acid derivative thiol unclassified drug water absorption article calculation chemical structure comparative study controlled study density electricity ellipsometry enzyme structure enzyme substrate film gravimetry impedance infrared spectroscopy molecular model priority journal surface property thickness water content Catechol Oxidase Electrostatics Gold Microchemistry Polyamines Quartz Spectroscopy, Fourier Transform Infrared Sulfonic Acids Surface Properties |
| spellingShingle |
Absorption spectroscopy Electric impedance Enzymes Fourier transform infrared spectroscopy Positive ions Self assembly Polycations Macromolecules 3 mercaptopropanesulfonic acid catechol oxidase enzyme gold poly(allylamine) poly(diallyldimethylamine) polycation polymer silicon dioxide sulfonic acid derivative thiol unclassified drug water absorption article calculation chemical structure comparative study controlled study density electricity ellipsometry enzyme structure enzyme substrate film gravimetry impedance infrared spectroscopy molecular model priority journal surface property thickness water content Catechol Oxidase Electrostatics Gold Microchemistry Polyamines Quartz Spectroscopy, Fourier Transform Infrared Sulfonic Acids Surface Properties Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase |
| topic_facet |
Absorption spectroscopy Electric impedance Enzymes Fourier transform infrared spectroscopy Positive ions Self assembly Polycations Macromolecules 3 mercaptopropanesulfonic acid catechol oxidase enzyme gold poly(allylamine) poly(diallyldimethylamine) polycation polymer silicon dioxide sulfonic acid derivative thiol unclassified drug water absorption article calculation chemical structure comparative study controlled study density electricity ellipsometry enzyme structure enzyme substrate film gravimetry impedance infrared spectroscopy molecular model priority journal surface property thickness water content Catechol Oxidase Electrostatics Gold Microchemistry Polyamines Quartz Spectroscopy, Fourier Transform Infrared Sulfonic Acids Surface Properties |
| description |
Self-assembled multilayers comprised of alternate layers of polyphenol oxidase (PPO) and poly(allylamine) (PAH) or PPO and poly(diallyldimethylamine) (PDDA), deposited on a 3-mercaptopropanesulfonic acid (MPS)-modified gold surface, were studied "in-situ" (under water) by means of ellipsometry and quartz crystal microbalance (QCM), and "ex-situ" (in open air) by ellipsometry and fourier transform infrared reflection-absorption spectroscopy (FT-IRRAS). Optical ellipsometric properties of (PAH n(PPO)n and (PDDA)n(PPO)n multilayers were obtained at two wavelengths, employing an isotropic single-layer model with the substrate parameters measured after thiol adsorption. Film thickness as well as ellipsometric mass values based on the de Feijter equation were also evaluated. The quartz crystal impedance analysis showed that self-assembled multilayers behaved as acoustically thin films, and therefore, the shifts observed in the film inductive impedance parameter were interpreted in terms of gravimetric mass. The enzyme mass uptake in each adsorption step was determined on PAH or on PDDA topmost layer. A comparative study between the ellipsometric thickness and acoustic mass values allowed us to obtain average values of "apparent" densities of (2.1 ± 0.1) and (2.4 ± 0.1) g cm-3 for (PAH)n(PPO n and (PDDA)n(PPO)n multilayers, respectively. The content of water included in the open polymer-enzyme structure was evaluated by comparison of QCM and ellipsometric mass values. FT-IRRAS spectra of each layer in (PAH)n(PPO n and (PDDA)n(PPO)n films were recorded, and the intensity ratio of the amide bands was evaluated to obtain information about layer-by-layer enzyme conformation. An enzyme/polycation distribution model for (PAH)n-(PPO)n and (PDDA)n(PPO)n multilayer structures is presented on the basis of combined ellipsometric, QCM, and FT-IRRAS results. |
| title |
Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase |
| title_short |
Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase |
| title_full |
Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase |
| title_fullStr |
Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase |
| title_full_unstemmed |
Effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase |
| title_sort |
effect of the polycation nature on the structure of layer-by-layer electrostatically self-assembled multilayers of polyphenol oxidase |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15257797_v4_n4_p869_Forzani http://hdl.handle.net/20.500.12110/paper_15257797_v4_n4_p869_Forzani |
| _version_ |
1768542662061719552 |