Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles

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The behavior of a fluorophore near a gold nanoparticle is rationalized by a theoretical description of the parameters that modify the fluorescence emission: nanoparticle-fluorophore distance, fluorescence quantum yield (φ0), and fluorophore absorption and emission spectra, to find optimum conditions...

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Autores principales: Estrada, Laura Cecilia, Roberti, Maria Julia, Simoncelli, Sabrina, Levi, Valeria, Aramendía, Pedro Francisco, Martínez, Oscar Eduardo
Publicado: 2012
Materias:
Emission spectroscopy
Fluorescence
Gold
Gold coatings
Luminance
Molecular biology
Molecules
Nanoparticles
Photobleaching
Quantum yield
Silicon compounds
Absorption and emission spectra
Core-shell nanoparticles
Fluorescence emission
Fluorescence quantum yield
Fluorescent molecules
Gold Nanoparticles
Imaging experiments
In-vitro
Isolated molecules
Maximum gain
Metallic nanoparticles
Optimum conditions
Photo-stability
Fluorophores
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15206106_v116_n7_p2306_Estrada
http://hdl.handle.net/20.500.12110/paper_15206106_v116_n7_p2306_Estrada
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_15206106_v116_n7_p2306_Estrada
record_format dspace
spelling paper:paper_15206106_v116_n7_p2306_Estrada2023-06-08T16:19:07Z Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles Estrada, Laura Cecilia Roberti, Maria Julia Simoncelli, Sabrina Levi, Valeria Aramendía, Pedro Francisco Martínez, Oscar Eduardo Emission spectroscopy Fluorescence Gold Gold coatings Luminance Molecular biology Molecules Nanoparticles Photobleaching Quantum yield Silicon compounds Absorption and emission spectra Core-shell nanoparticles Fluorescence emission Fluorescence quantum yield Fluorescent molecules Gold Nanoparticles Imaging experiments In-vitro Isolated molecules Maximum gain Metallic nanoparticles Optimum conditions Photo-stability Fluorophores The behavior of a fluorophore near a gold nanoparticle is rationalized by a theoretical description of the parameters that modify the fluorescence emission: nanoparticle-fluorophore distance, fluorescence quantum yield (φ0), and fluorophore absorption and emission spectra, to find optimum conditions for designing fluorophore-nanoparticle probes. The theoretical maximum gain in brightness of the nanoparticle-fluorophore system with respect to the isolated molecule increases almost inversely proportional to φ0. The brightness enhancement in imaging experiments in vitro was assessed by using Au-SiO2 core-shell nanoparticles deposited on glass. A ∼13-fold emission brightness enhancement for weakly fluorescent molecules was observed. A significant increase in fluorophore photostability, rendering longer imaging times, was obtained for fluorophores interacting with gold nanoparticles incorporated by endocytosis in cells. Our results illustrate a way to increase imaging times and to study molecules in the vicinity of a metallic nanoparticle after photobleaching of background fluorescence. © 2012 American Chemical Society. Fil:Estrada, L.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roberti, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Simoncelli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aramendía, P.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15206106_v116_n7_p2306_Estrada http://hdl.handle.net/20.500.12110/paper_15206106_v116_n7_p2306_Estrada
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Emission spectroscopy
Fluorescence
Gold
Gold coatings
Luminance
Molecular biology
Molecules
Nanoparticles
Photobleaching
Quantum yield
Silicon compounds
Absorption and emission spectra
Core-shell nanoparticles
Fluorescence emission
Fluorescence quantum yield
Fluorescent molecules
Gold Nanoparticles
Imaging experiments
In-vitro
Isolated molecules
Maximum gain
Metallic nanoparticles
Optimum conditions
Photo-stability
Fluorophores
spellingShingle Emission spectroscopy
Fluorescence
Gold
Gold coatings
Luminance
Molecular biology
Molecules
Nanoparticles
Photobleaching
Quantum yield
Silicon compounds
Absorption and emission spectra
Core-shell nanoparticles
Fluorescence emission
Fluorescence quantum yield
Fluorescent molecules
Gold Nanoparticles
Imaging experiments
In-vitro
Isolated molecules
Maximum gain
Metallic nanoparticles
Optimum conditions
Photo-stability
Fluorophores
Estrada, Laura Cecilia
Roberti, Maria Julia
Simoncelli, Sabrina
Levi, Valeria
Aramendía, Pedro Francisco
Martínez, Oscar Eduardo
Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
topic_facet Emission spectroscopy
Fluorescence
Gold
Gold coatings
Luminance
Molecular biology
Molecules
Nanoparticles
Photobleaching
Quantum yield
Silicon compounds
Absorption and emission spectra
Core-shell nanoparticles
Fluorescence emission
Fluorescence quantum yield
Fluorescent molecules
Gold Nanoparticles
Imaging experiments
In-vitro
Isolated molecules
Maximum gain
Metallic nanoparticles
Optimum conditions
Photo-stability
Fluorophores
description The behavior of a fluorophore near a gold nanoparticle is rationalized by a theoretical description of the parameters that modify the fluorescence emission: nanoparticle-fluorophore distance, fluorescence quantum yield (φ0), and fluorophore absorption and emission spectra, to find optimum conditions for designing fluorophore-nanoparticle probes. The theoretical maximum gain in brightness of the nanoparticle-fluorophore system with respect to the isolated molecule increases almost inversely proportional to φ0. The brightness enhancement in imaging experiments in vitro was assessed by using Au-SiO2 core-shell nanoparticles deposited on glass. A ∼13-fold emission brightness enhancement for weakly fluorescent molecules was observed. A significant increase in fluorophore photostability, rendering longer imaging times, was obtained for fluorophores interacting with gold nanoparticles incorporated by endocytosis in cells. Our results illustrate a way to increase imaging times and to study molecules in the vicinity of a metallic nanoparticle after photobleaching of background fluorescence. © 2012 American Chemical Society.
author Estrada, Laura Cecilia
Roberti, Maria Julia
Simoncelli, Sabrina
Levi, Valeria
Aramendía, Pedro Francisco
Martínez, Oscar Eduardo
author_facet Estrada, Laura Cecilia
Roberti, Maria Julia
Simoncelli, Sabrina
Levi, Valeria
Aramendía, Pedro Francisco
Martínez, Oscar Eduardo
author_sort Estrada, Laura Cecilia
title Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
title_short Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
title_full Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
title_fullStr Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
title_full_unstemmed Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
title_sort detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15206106_v116_n7_p2306_Estrada
http://hdl.handle.net/20.500.12110/paper_15206106_v116_n7_p2306_Estrada
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