Expression of the TGF-beta1 system in human testicular pathologies

Background: In non-obstructive azoospermia, histological patterns of Sertoli cell-only Syndrome (SCO) and hypospermatogenesis (H) are commonly found. In these pathologies, Leydig cell hyperplasia (LCH) is detected in some patients. Since TGF-β1 is involved in cellular proliferation/development, the...

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Publicado: 2010
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14777827_v8_n_p_Gonzalez
http://hdl.handle.net/20.500.12110/paper_14777827_v8_n_p_Gonzalez
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spelling paper:paper_14777827_v8_n_p_Gonzalez2023-06-08T16:18:10Z Expression of the TGF-beta1 system in human testicular pathologies endoglin messenger RNA Smad protein Smad1 protein Smad2 protein Smad3 protein Smad5 protein transforming growth factor beta receptor 1 transforming growth factor beta receptor 2 transforming growth factor beta1 activin receptor 2 ACVRL1 protein, human cell surface receptor ENG protein, human leukocyte antigen messenger RNA protein serine threonine kinase Smad protein TGF beta type I receptor TGF-beta type I receptor transforming growth factor beta receptor transforming growth factor beta type II receptor transforming growth factor beta1 transforming growth factor-beta type II receptor adult article clinical article gene expression human human tissue hypospermatogenesis immunopathology infertility Leydig cell male nucleotide sequence polymerase chain reaction Sertoli cell only syndrome testis disease biosynthesis hyperplasia male infertility metabolism middle aged pathology testis Activin Receptors, Type II Adult Antigens, CD Humans Hyperplasia Infertility, Male Leydig Cells Male Middle Aged Protein-Serine-Threonine Kinases Receptors, Cell Surface Receptors, Transforming Growth Factor beta RNA, Messenger Sertoli Cell-Only Syndrome Smad Proteins Testicular Diseases Testis Transforming Growth Factor beta1 Background: In non-obstructive azoospermia, histological patterns of Sertoli cell-only Syndrome (SCO) and hypospermatogenesis (H) are commonly found. In these pathologies, Leydig cell hyperplasia (LCH) is detected in some patients. Since TGF-β1 is involved in cellular proliferation/development, the aim of this work was to analyze the expression of TGF-β1, its receptors TGFBRII, TGFBRI (ALK-1 and ALK-5), and the co-receptor endoglin in human biopsies from patients with idiopathic infertility.Methods: Specific immunostaining of TGF-β1, its receptors TGFBRII, TGFBRI (ALK-1 and ALK-5), co-receptor endoglin and Smads proteins, were carried out in testicular biopsies from normal and infertile men with SCO or H. Gene expression of TGF-β1 system were made in biopsies from infertile patients with semi-quantitative and quantitative PCR.Results: Immunohistochemical studies revealed that TGF-β1 and its specific receptors are present in Leydig cells in biopsies from normal tissue or patients with SCO or H with or without LCH. Smad proteins, which are involved in TGF-β1 signaling, are also detected in both their phosphorylated (activated) and dephosphorylated form in all samples TGF-β1, ALK-1 and endoglin gene expression are stronger in human biopsies with LCH than in those with SCO or H. Neither TGFBRII nor ALK-5 gene expression showed significant differences between pathologies. A significant correlation between ALK-1 and endoglin expression was observed.Conclusions: In conclusion, the high levels of mRNA and protein expression of the TGF-β1 system in patients with LCH, particularly ALK1 and its correlation with endoglin, suggest that these proteins acting in concert might be, at least in part, committed actors in the Leydig cell hyperplasia. © 2010 Gonzalez et al; licensee BioMed Central Ltd. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14777827_v8_n_p_Gonzalez http://hdl.handle.net/20.500.12110/paper_14777827_v8_n_p_Gonzalez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic endoglin
messenger RNA
Smad protein
Smad1 protein
Smad2 protein
Smad3 protein
Smad5 protein
transforming growth factor beta receptor 1
transforming growth factor beta receptor 2
transforming growth factor beta1
activin receptor 2
ACVRL1 protein, human
cell surface receptor
ENG protein, human
leukocyte antigen
messenger RNA
protein serine threonine kinase
Smad protein
TGF beta type I receptor
TGF-beta type I receptor
transforming growth factor beta receptor
transforming growth factor beta type II receptor
transforming growth factor beta1
transforming growth factor-beta type II receptor
adult
article
clinical article
gene expression
human
human tissue
hypospermatogenesis
immunopathology
infertility
Leydig cell
male
nucleotide sequence
polymerase chain reaction
Sertoli cell only syndrome
testis disease
biosynthesis
hyperplasia
male infertility
metabolism
middle aged
pathology
testis
Activin Receptors, Type II
Adult
Antigens, CD
Humans
Hyperplasia
Infertility, Male
Leydig Cells
Male
Middle Aged
Protein-Serine-Threonine Kinases
Receptors, Cell Surface
Receptors, Transforming Growth Factor beta
RNA, Messenger
Sertoli Cell-Only Syndrome
Smad Proteins
Testicular Diseases
Testis
Transforming Growth Factor beta1
spellingShingle endoglin
messenger RNA
Smad protein
Smad1 protein
Smad2 protein
Smad3 protein
Smad5 protein
transforming growth factor beta receptor 1
transforming growth factor beta receptor 2
transforming growth factor beta1
activin receptor 2
ACVRL1 protein, human
cell surface receptor
ENG protein, human
leukocyte antigen
messenger RNA
protein serine threonine kinase
Smad protein
TGF beta type I receptor
TGF-beta type I receptor
transforming growth factor beta receptor
transforming growth factor beta type II receptor
transforming growth factor beta1
transforming growth factor-beta type II receptor
adult
article
clinical article
gene expression
human
human tissue
hypospermatogenesis
immunopathology
infertility
Leydig cell
male
nucleotide sequence
polymerase chain reaction
Sertoli cell only syndrome
testis disease
biosynthesis
hyperplasia
male infertility
metabolism
middle aged
pathology
testis
Activin Receptors, Type II
Adult
Antigens, CD
Humans
Hyperplasia
Infertility, Male
Leydig Cells
Male
Middle Aged
Protein-Serine-Threonine Kinases
Receptors, Cell Surface
Receptors, Transforming Growth Factor beta
RNA, Messenger
Sertoli Cell-Only Syndrome
Smad Proteins
Testicular Diseases
Testis
Transforming Growth Factor beta1
Expression of the TGF-beta1 system in human testicular pathologies
topic_facet endoglin
messenger RNA
Smad protein
Smad1 protein
Smad2 protein
Smad3 protein
Smad5 protein
transforming growth factor beta receptor 1
transforming growth factor beta receptor 2
transforming growth factor beta1
activin receptor 2
ACVRL1 protein, human
cell surface receptor
ENG protein, human
leukocyte antigen
messenger RNA
protein serine threonine kinase
Smad protein
TGF beta type I receptor
TGF-beta type I receptor
transforming growth factor beta receptor
transforming growth factor beta type II receptor
transforming growth factor beta1
transforming growth factor-beta type II receptor
adult
article
clinical article
gene expression
human
human tissue
hypospermatogenesis
immunopathology
infertility
Leydig cell
male
nucleotide sequence
polymerase chain reaction
Sertoli cell only syndrome
testis disease
biosynthesis
hyperplasia
male infertility
metabolism
middle aged
pathology
testis
Activin Receptors, Type II
Adult
Antigens, CD
Humans
Hyperplasia
Infertility, Male
Leydig Cells
Male
Middle Aged
Protein-Serine-Threonine Kinases
Receptors, Cell Surface
Receptors, Transforming Growth Factor beta
RNA, Messenger
Sertoli Cell-Only Syndrome
Smad Proteins
Testicular Diseases
Testis
Transforming Growth Factor beta1
description Background: In non-obstructive azoospermia, histological patterns of Sertoli cell-only Syndrome (SCO) and hypospermatogenesis (H) are commonly found. In these pathologies, Leydig cell hyperplasia (LCH) is detected in some patients. Since TGF-β1 is involved in cellular proliferation/development, the aim of this work was to analyze the expression of TGF-β1, its receptors TGFBRII, TGFBRI (ALK-1 and ALK-5), and the co-receptor endoglin in human biopsies from patients with idiopathic infertility.Methods: Specific immunostaining of TGF-β1, its receptors TGFBRII, TGFBRI (ALK-1 and ALK-5), co-receptor endoglin and Smads proteins, were carried out in testicular biopsies from normal and infertile men with SCO or H. Gene expression of TGF-β1 system were made in biopsies from infertile patients with semi-quantitative and quantitative PCR.Results: Immunohistochemical studies revealed that TGF-β1 and its specific receptors are present in Leydig cells in biopsies from normal tissue or patients with SCO or H with or without LCH. Smad proteins, which are involved in TGF-β1 signaling, are also detected in both their phosphorylated (activated) and dephosphorylated form in all samples TGF-β1, ALK-1 and endoglin gene expression are stronger in human biopsies with LCH than in those with SCO or H. Neither TGFBRII nor ALK-5 gene expression showed significant differences between pathologies. A significant correlation between ALK-1 and endoglin expression was observed.Conclusions: In conclusion, the high levels of mRNA and protein expression of the TGF-β1 system in patients with LCH, particularly ALK1 and its correlation with endoglin, suggest that these proteins acting in concert might be, at least in part, committed actors in the Leydig cell hyperplasia. © 2010 Gonzalez et al; licensee BioMed Central Ltd.
title Expression of the TGF-beta1 system in human testicular pathologies
title_short Expression of the TGF-beta1 system in human testicular pathologies
title_full Expression of the TGF-beta1 system in human testicular pathologies
title_fullStr Expression of the TGF-beta1 system in human testicular pathologies
title_full_unstemmed Expression of the TGF-beta1 system in human testicular pathologies
title_sort expression of the tgf-beta1 system in human testicular pathologies
publishDate 2010
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14777827_v8_n_p_Gonzalez
http://hdl.handle.net/20.500.12110/paper_14777827_v8_n_p_Gonzalez
_version_ 1768543673237110784