Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method

Reflectance spectra from pink petals of Rhododendron indicum flowers showed absorption in the NIR (1470, 1930 and 2500 nm) due to water, in the visible (533 nm, due to anthocyanins) and in the UV (broad absorption due to phenolic compounds other than anthocyanins). A linear correlation between the r...

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Autores principales:	Iriel, Analía, Lagorio, María Gabriela
Publicado:	2009
Materias:	anthocyanin flavonoid phenol derivative absorption article flower fluorescence nonhuman petal priority journal reflectometry Rhododendron spectroscopy ultraviolet radiation Anthocyanins Flavonoids Flowers Phenols Pigments, Biological Spectrometry, Fluorescence Rhododendron indicum
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v8_n3_p337_Iriel http://hdl.handle.net/20.500.12110/paper_1474905X_v8_n3_p337_Iriel
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1474905X_v8_n3_p337_Iriel
record_format	dspace
spelling	paper:paper_1474905X_v8_n3_p337_Iriel2023-06-08T16:17:48Z Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method Iriel, Analía Lagorio, María Gabriela anthocyanin flavonoid phenol derivative absorption article flower fluorescence nonhuman petal priority journal reflectometry Rhododendron spectroscopy ultraviolet radiation Anthocyanins Flavonoids Flowers Phenols Pigments, Biological Rhododendron Spectrometry, Fluorescence Rhododendron indicum Reflectance spectra from pink petals of Rhododendron indicum flowers showed absorption in the NIR (1470, 1930 and 2500 nm) due to water, in the visible (533 nm, due to anthocyanins) and in the UV (broad absorption due to phenolic compounds other than anthocyanins). A linear correlation between the remission function at 533 nm and the anthocyanin content in μmol per g fresh weight has been found, allowing non-destructive quantification of anthocyanins. The remission function could be obtained either from reflectance of a group of stacked petals (Kubelka-Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. The intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm under UV excitation. The red emission was attributed to anthocyanins whereas blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of crude and purified extracts from the petals, the last compounds could possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or others. © 2009 The Royal Society of Chemistry and Owner Societies. Fil:Iriel, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lagorio, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v8_n3_p337_Iriel http://hdl.handle.net/20.500.12110/paper_1474905X_v8_n3_p337_Iriel
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	anthocyanin flavonoid phenol derivative absorption article flower fluorescence nonhuman petal priority journal reflectometry Rhododendron spectroscopy ultraviolet radiation Anthocyanins Flavonoids Flowers Phenols Pigments, Biological Rhododendron Spectrometry, Fluorescence Rhododendron indicum
spellingShingle	anthocyanin flavonoid phenol derivative absorption article flower fluorescence nonhuman petal priority journal reflectometry Rhododendron spectroscopy ultraviolet radiation Anthocyanins Flavonoids Flowers Phenols Pigments, Biological Rhododendron Spectrometry, Fluorescence Rhododendron indicum Iriel, Analía Lagorio, María Gabriela Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method
topic_facet	anthocyanin flavonoid phenol derivative absorption article flower fluorescence nonhuman petal priority journal reflectometry Rhododendron spectroscopy ultraviolet radiation Anthocyanins Flavonoids Flowers Phenols Pigments, Biological Rhododendron Spectrometry, Fluorescence Rhododendron indicum
description	Reflectance spectra from pink petals of Rhododendron indicum flowers showed absorption in the NIR (1470, 1930 and 2500 nm) due to water, in the visible (533 nm, due to anthocyanins) and in the UV (broad absorption due to phenolic compounds other than anthocyanins). A linear correlation between the remission function at 533 nm and the anthocyanin content in μmol per g fresh weight has been found, allowing non-destructive quantification of anthocyanins. The remission function could be obtained either from reflectance of a group of stacked petals (Kubelka-Munk theory) or through determination of the absorption and scattering coefficients following the Pile of Plates model. The intact petals have shown fluorescence emission in the blue (400-500 nm) and in the visible around 624 nm under UV excitation. The red emission was attributed to anthocyanins whereas blue emission was assigned to other phenolic compounds. On the basis of absorption and fluorescence measurements of crude and purified extracts from the petals, the last compounds could possibly be a mixture of flavonoids and hydroxycinnamic-type plant phenolics such as ferulic acid, chlorogenic acid or others. © 2009 The Royal Society of Chemistry and Owner Societies.
author	Iriel, Analía Lagorio, María Gabriela
author_facet	Iriel, Analía Lagorio, María Gabriela
author_sort	Iriel, Analía
title	Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method
title_short	Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method
title_full	Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method
title_fullStr	Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method
title_full_unstemmed	Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method
title_sort	biospectroscopy of rhododendron indicum flowers. non-destructive assessment of anthocyanins in petals using a reflectance-based method
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v8_n3_p337_Iriel http://hdl.handle.net/20.500.12110/paper_1474905X_v8_n3_p337_Iriel
work_keys_str_mv	AT irielanalia biospectroscopyofrhododendronindicumflowersnondestructiveassessmentofanthocyaninsinpetalsusingareflectancebasedmethod AT lagoriomariagabriela biospectroscopyofrhododendronindicumflowersnondestructiveassessmentofanthocyaninsinpetalsusingareflectancebasedmethod
_version_	1768546738849710080

Biospectroscopy of Rhododendron indicum flowers. Non-destructive assessment of anthocyanins in petals using a reflectance-based method

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