Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators

We prepared water soluble, biocompatible fluorescent turn-on pH nanosensors and characterized their behavior as a function of changes in pH. The response relies on a halochromic reaction of a spirorhodamineamide derived from the bright and highly chemically and photo-stable rhodamine 6G, encapsulate...

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Publicado: 2019
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v18_n1_p155_DiPaolo
http://hdl.handle.net/20.500.12110/paper_1474905X_v18_n1_p155_DiPaolo
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_1474905X_v18_n1_p155_DiPaolo
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spelling paper:paper_1474905X_v18_n1_p155_DiPaolo2023-06-08T16:17:42Z Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators We prepared water soluble, biocompatible fluorescent turn-on pH nanosensors and characterized their behavior as a function of changes in pH. The response relies on a halochromic reaction of a spirorhodamineamide derived from the bright and highly chemically and photo-stable rhodamine 6G, encapsulated in core/nanoporous shell silica nanoparticles. The fluorescent sensors displayed a fast response in the pH range of intracellular compartments. The encapsulation conferred solubility in aqueous environments and biocompatibility. We assessed the two main properties of the sensor, namely the useful pH range and the kinetics of the response, and compared them to those of the free probe. We found that such properties are strongly dependent on the functionalization and position in the silica matrix relative to the core/shell structure. Finally, we demonstrated the cellular uptake of the nanosensors, and their localization in lysosomes of living cells, by fluorescence confocal microscopy. © The Royal Society of Chemistry and Owner Societies. 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v18_n1_p155_DiPaolo http://hdl.handle.net/20.500.12110/paper_1474905X_v18_n1_p155_DiPaolo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description We prepared water soluble, biocompatible fluorescent turn-on pH nanosensors and characterized their behavior as a function of changes in pH. The response relies on a halochromic reaction of a spirorhodamineamide derived from the bright and highly chemically and photo-stable rhodamine 6G, encapsulated in core/nanoporous shell silica nanoparticles. The fluorescent sensors displayed a fast response in the pH range of intracellular compartments. The encapsulation conferred solubility in aqueous environments and biocompatibility. We assessed the two main properties of the sensor, namely the useful pH range and the kinetics of the response, and compared them to those of the free probe. We found that such properties are strongly dependent on the functionalization and position in the silica matrix relative to the core/shell structure. Finally, we demonstrated the cellular uptake of the nanosensors, and their localization in lysosomes of living cells, by fluorescence confocal microscopy. © The Royal Society of Chemistry and Owner Societies.
title Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators
spellingShingle Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators
title_short Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators
title_full Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators
title_fullStr Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators
title_full_unstemmed Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators
title_sort nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as ph indicators
publishDate 2019
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1474905X_v18_n1_p155_DiPaolo
http://hdl.handle.net/20.500.12110/paper_1474905X_v18_n1_p155_DiPaolo
_version_ 1768542380228608000

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