Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.

BACKGROUND: Acute Intermittent Porphyria is a genetic disorder of heme metabolism, characterized by increased levels of porphyrin precursors, delta-aminolevulinic acid (ALA) and porphobilinogen (PBG). ALA has been reported to generate reactive oxygen species and to cause oxidative damage to proteins...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	De Siervi, Adriana, Vázquez, Elba Susana, Rezával, Carolina, Rossetti, María Victoria, Batlle, Alcira María del Carmen
Publicado:	2002
Materias:	aminolevulinic acid DNA DNA fragment glucose hemin protein p53 tetrazolium thiazole derivative thiazolyl blue article biosynthesis cell culture cell cycle dose response drug effect human liver cell carcinoma liver tumor metabolism Aminolevulinic Acid Carcinoma, Hepatocellular Cell Cycle DNA Fragmentation DNA, Neoplasm Dose-Response Relationship, Drug Glucose Hemin Humans Liver Neoplasms Tetrazolium Salts Thiazoles Tumor Cells, Cultured Tumor Suppressor Protein p53
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712407_v2_n1_p6_DeSiervi http://hdl.handle.net/20.500.12110/paper_14712407_v2_n1_p6_DeSiervi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14712407_v2_n1_p6_DeSiervi
record_format	dspace
spelling	paper:paper_14712407_v2_n1_p6_DeSiervi2023-06-08T16:17:20Z Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines. De Siervi, Adriana Vázquez, Elba Susana Rezával, Carolina Rossetti, María Victoria Batlle, Alcira María del Carmen aminolevulinic acid DNA DNA fragment glucose hemin protein p53 tetrazolium thiazole derivative thiazolyl blue article biosynthesis cell culture cell cycle dose response drug effect human liver cell carcinoma liver tumor metabolism Aminolevulinic Acid Carcinoma, Hepatocellular Cell Cycle DNA Fragmentation DNA, Neoplasm Dose-Response Relationship, Drug Glucose Hemin Humans Liver Neoplasms Tetrazolium Salts Thiazoles Tumor Cells, Cultured Tumor Suppressor Protein p53 BACKGROUND: Acute Intermittent Porphyria is a genetic disorder of heme metabolism, characterized by increased levels of porphyrin precursors, delta-aminolevulinic acid (ALA) and porphobilinogen (PBG). ALA has been reported to generate reactive oxygen species and to cause oxidative damage to proteins, subcellular structures and DNA. It is known that oxidative stress can induce apoptosis. The aim of this work was to study the cytotoxic effect of ALA on two hepatocarcinoma cell lines. RESULTS: We have determined the impact of ALA on HEP G2 and HEP 3B hepatocarcinoma cell lines survival as measured by the MTT assay. ALA proved to be cytotoxic in both cell lines however; HEP G2 was more sensitive to ALA than HEP 3B. Addition of hemin or glucose diminished ALA cytotoxicity in HEP G2 cells; instead it was enhanced in HEP 3B cells. Because apoptosis is usually associated with DNA fragmentation, the DNA of ALA treated and untreated cells were analyzed. The characteristic pattern of DNA fragmentation ladders was observed in ALA treated cells. To elucidate the mechanisms of ALA induced apoptosis, we examined its effect on p53 expression. No changes in p53 mRNA levels were observed after exposure of both cell lines to ALA for 24 h. CDK2 and CDK4 protein levels were reduced after ALA treatment at physiological concentrations. Fil:De Siervi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rezaval, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712407_v2_n1_p6_DeSiervi http://hdl.handle.net/20.500.12110/paper_14712407_v2_n1_p6_DeSiervi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	aminolevulinic acid DNA DNA fragment glucose hemin protein p53 tetrazolium thiazole derivative thiazolyl blue article biosynthesis cell culture cell cycle dose response drug effect human liver cell carcinoma liver tumor metabolism Aminolevulinic Acid Carcinoma, Hepatocellular Cell Cycle DNA Fragmentation DNA, Neoplasm Dose-Response Relationship, Drug Glucose Hemin Humans Liver Neoplasms Tetrazolium Salts Thiazoles Tumor Cells, Cultured Tumor Suppressor Protein p53
spellingShingle	aminolevulinic acid DNA DNA fragment glucose hemin protein p53 tetrazolium thiazole derivative thiazolyl blue article biosynthesis cell culture cell cycle dose response drug effect human liver cell carcinoma liver tumor metabolism Aminolevulinic Acid Carcinoma, Hepatocellular Cell Cycle DNA Fragmentation DNA, Neoplasm Dose-Response Relationship, Drug Glucose Hemin Humans Liver Neoplasms Tetrazolium Salts Thiazoles Tumor Cells, Cultured Tumor Suppressor Protein p53 De Siervi, Adriana Vázquez, Elba Susana Rezával, Carolina Rossetti, María Victoria Batlle, Alcira María del Carmen Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.
topic_facet	aminolevulinic acid DNA DNA fragment glucose hemin protein p53 tetrazolium thiazole derivative thiazolyl blue article biosynthesis cell culture cell cycle dose response drug effect human liver cell carcinoma liver tumor metabolism Aminolevulinic Acid Carcinoma, Hepatocellular Cell Cycle DNA Fragmentation DNA, Neoplasm Dose-Response Relationship, Drug Glucose Hemin Humans Liver Neoplasms Tetrazolium Salts Thiazoles Tumor Cells, Cultured Tumor Suppressor Protein p53
description	BACKGROUND: Acute Intermittent Porphyria is a genetic disorder of heme metabolism, characterized by increased levels of porphyrin precursors, delta-aminolevulinic acid (ALA) and porphobilinogen (PBG). ALA has been reported to generate reactive oxygen species and to cause oxidative damage to proteins, subcellular structures and DNA. It is known that oxidative stress can induce apoptosis. The aim of this work was to study the cytotoxic effect of ALA on two hepatocarcinoma cell lines. RESULTS: We have determined the impact of ALA on HEP G2 and HEP 3B hepatocarcinoma cell lines survival as measured by the MTT assay. ALA proved to be cytotoxic in both cell lines however; HEP G2 was more sensitive to ALA than HEP 3B. Addition of hemin or glucose diminished ALA cytotoxicity in HEP G2 cells; instead it was enhanced in HEP 3B cells. Because apoptosis is usually associated with DNA fragmentation, the DNA of ALA treated and untreated cells were analyzed. The characteristic pattern of DNA fragmentation ladders was observed in ALA treated cells. To elucidate the mechanisms of ALA induced apoptosis, we examined its effect on p53 expression. No changes in p53 mRNA levels were observed after exposure of both cell lines to ALA for 24 h. CDK2 and CDK4 protein levels were reduced after ALA treatment at physiological concentrations.
author	De Siervi, Adriana Vázquez, Elba Susana Rezával, Carolina Rossetti, María Victoria Batlle, Alcira María del Carmen
author_facet	De Siervi, Adriana Vázquez, Elba Susana Rezával, Carolina Rossetti, María Victoria Batlle, Alcira María del Carmen
author_sort	De Siervi, Adriana
title	Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.
title_short	Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.
title_full	Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.
title_fullStr	Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.
title_full_unstemmed	Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.
title_sort	delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712407_v2_n1_p6_DeSiervi http://hdl.handle.net/20.500.12110/paper_14712407_v2_n1_p6_DeSiervi
work_keys_str_mv	AT desierviadriana deltaaminolevulinicacidcytotoxiceffectsonhumanhepatocarcinomacelllines AT vazquezelbasusana deltaaminolevulinicacidcytotoxiceffectsonhumanhepatocarcinomacelllines AT rezavalcarolina deltaaminolevulinicacidcytotoxiceffectsonhumanhepatocarcinomacelllines AT rossettimariavictoria deltaaminolevulinicacidcytotoxiceffectsonhumanhepatocarcinomacelllines AT batllealciramariadelcarmen deltaaminolevulinicacidcytotoxiceffectsonhumanhepatocarcinomacelllines
_version_	1768544289142341632

Delta-aminolevulinic acid cytotoxic effects on human hepatocarcinoma cell lines.

Ejemplares similares