Determination of the molecular size of BSA by fluorescence anisotropy
This work describes a laboratory experiment to illustrate the usefulness of fluorescence anisotropy in the field of biophysics. Fluorescence anisotropy of dansyl-labeled BSA was determined in media of increasing glycerol concentrations. The Perrin equation was fitted to the experimental data, obtain...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14708175_v31_n5_p319_Flecha http://hdl.handle.net/20.500.12110/paper_14708175_v31_n5_p319_Flecha |
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paper:paper_14708175_v31_n5_p319_Flecha |
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paper:paper_14708175_v31_n5_p319_Flecha2023-06-08T16:17:09Z Determination of the molecular size of BSA by fluorescence anisotropy BSA Fluorescence anisotropy Molecular size glycerol protein protein BSA unclassified drug anisotropy article biophysics data analysis fluorescence analysis molecular probe molecular size university student This work describes a laboratory experiment to illustrate the usefulness of fluorescence anisotropy in the field of biophysics. Fluorescence anisotropy of dansyl-labeled BSA was determined in media of increasing glycerol concentrations. The Perrin equation was fitted to the experimental data, obtaining the molecular volume of the protein. The simplicity of the experiment and data analysis helped the students to focus on the relationship between probe anisotropy and rotational diffusion. Additionally, this laboratory exercise has the advantage of using a protein and a probe that are inexpensive and very common in many laboratories. © 2003 by The International Union of Biochemistry and Molecular Biology. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14708175_v31_n5_p319_Flecha http://hdl.handle.net/20.500.12110/paper_14708175_v31_n5_p319_Flecha |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
BSA Fluorescence anisotropy Molecular size glycerol protein protein BSA unclassified drug anisotropy article biophysics data analysis fluorescence analysis molecular probe molecular size university student |
| spellingShingle |
BSA Fluorescence anisotropy Molecular size glycerol protein protein BSA unclassified drug anisotropy article biophysics data analysis fluorescence analysis molecular probe molecular size university student Determination of the molecular size of BSA by fluorescence anisotropy |
| topic_facet |
BSA Fluorescence anisotropy Molecular size glycerol protein protein BSA unclassified drug anisotropy article biophysics data analysis fluorescence analysis molecular probe molecular size university student |
| description |
This work describes a laboratory experiment to illustrate the usefulness of fluorescence anisotropy in the field of biophysics. Fluorescence anisotropy of dansyl-labeled BSA was determined in media of increasing glycerol concentrations. The Perrin equation was fitted to the experimental data, obtaining the molecular volume of the protein. The simplicity of the experiment and data analysis helped the students to focus on the relationship between probe anisotropy and rotational diffusion. Additionally, this laboratory exercise has the advantage of using a protein and a probe that are inexpensive and very common in many laboratories. © 2003 by The International Union of Biochemistry and Molecular Biology. |
| title |
Determination of the molecular size of BSA by fluorescence anisotropy |
| title_short |
Determination of the molecular size of BSA by fluorescence anisotropy |
| title_full |
Determination of the molecular size of BSA by fluorescence anisotropy |
| title_fullStr |
Determination of the molecular size of BSA by fluorescence anisotropy |
| title_full_unstemmed |
Determination of the molecular size of BSA by fluorescence anisotropy |
| title_sort |
determination of the molecular size of bsa by fluorescence anisotropy |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14708175_v31_n5_p319_Flecha http://hdl.handle.net/20.500.12110/paper_14708175_v31_n5_p319_Flecha |
| _version_ |
1768545343602950144 |