Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients
Sperm protein tyrosine phosphorylation has been associated with capacitation, motility changes, zona binding, and fertilizing ability. We previously demonstrated that gradient-isolated human sperm subpopulations differ in their plasma membrane composition, their ability to phosphorylate proteins in...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v129_n6_p697_Buffone http://hdl.handle.net/20.500.12110/paper_14701626_v129_n6_p697_Buffone |
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paper:paper_14701626_v129_n6_p697_Buffone2023-06-08T16:17:02Z Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients cyclic AMP laurdan phosphodiesterase inhibitor tyrosine adult article asthenospermia cell activation cell isolation cell membrane cell subpopulation clinical article computer analysis controlled study disease association fertilization fluorescence analysis human human cell hypothesis immunofluorescence incubation time male male fertility male infertility membrane binding membrane fluidity membrane permeability pathogenesis polarization priority journal protein phosphorylation semen analysis sperm donor spermatozoon capacitation spermatozoon count spermatozoon motility velocity Western blotting zona pellucida Adult Analysis of Variance Blotting, Western Bucladesine Case-Control Studies Cells, Cultured Fluorescent Antibody Technique, Indirect Humans Image Processing, Computer-Assisted Male Membrane Fluidity Microscopy, Fluorescence Oligospermia Pentoxifylline Phosphodiesterase Inhibitors Phosphorylation Phosphotyrosine Sperm Capacitation Sperm Motility Spermatozoa Tyrosine Sperm protein tyrosine phosphorylation has been associated with capacitation, motility changes, zona binding, and fertilizing ability. We previously demonstrated that gradient-isolated human sperm subpopulations differ in their plasma membrane composition, their ability to phosphorylate proteins in tyrosine residues, and their capacity to undergo hyperactivation. In this study, we have characterized capacitation-associated changes in protein tyrosine phosphorylation and membrane fluidity in spermatozoa of asthenozoospermic and normozoospermic patients consulting for infertility. Semen samples were studied at baseline and after a capacitating incubation with or without the addition of a permeable cAMP analog and a phosphodiesterase inhibitor. Basic sperm and computer-assisted motion parameters, hyperactivation, protein tyrosine phosphorylation (immunofluorescence and Western blot), and membrane fluidity (fluorescent Laurdan probe) were the main study parameters. In comparison with normozoospermic and proven-fertile donor semen, asthenozoospermic samples showed lower motility, velocity, and amplitude of lateral head displacement, both originally and after a 6-h capacitating incubation. Unlike those in normal samples, asthenozoospermic spermatozoa were unable to increase protein tyrosine phosphorylation during capacitation. Such impairment, however, was overcome when they were incubated with a membrane-permeable cAMP analog and a phosphodiesterase inhibitor, indicating a possible membrane defect. Confirming this hypothesis, plasma membranes of asthenozoospermic sperm showed decreased fluidity (increased Laurdan polarization), even after a capacitating incubation. In conclusion, spermatozoa from functional asthenozoospermic samples may owe their poor motility, and their inability to properly capacitate and develop hyperactivation, to an impairment in the tyrosine phosphorylation of critical proteins caused by decreased membrane fluidity. These findings suggest a molecular pathogenetic mechanism for a common seminal pathology associated with male infertility. © 2005 Society for Reproduction and Fertility. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v129_n6_p697_Buffone http://hdl.handle.net/20.500.12110/paper_14701626_v129_n6_p697_Buffone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cyclic AMP laurdan phosphodiesterase inhibitor tyrosine adult article asthenospermia cell activation cell isolation cell membrane cell subpopulation clinical article computer analysis controlled study disease association fertilization fluorescence analysis human human cell hypothesis immunofluorescence incubation time male male fertility male infertility membrane binding membrane fluidity membrane permeability pathogenesis polarization priority journal protein phosphorylation semen analysis sperm donor spermatozoon capacitation spermatozoon count spermatozoon motility velocity Western blotting zona pellucida Adult Analysis of Variance Blotting, Western Bucladesine Case-Control Studies Cells, Cultured Fluorescent Antibody Technique, Indirect Humans Image Processing, Computer-Assisted Male Membrane Fluidity Microscopy, Fluorescence Oligospermia Pentoxifylline Phosphodiesterase Inhibitors Phosphorylation Phosphotyrosine Sperm Capacitation Sperm Motility Spermatozoa Tyrosine |
| spellingShingle |
cyclic AMP laurdan phosphodiesterase inhibitor tyrosine adult article asthenospermia cell activation cell isolation cell membrane cell subpopulation clinical article computer analysis controlled study disease association fertilization fluorescence analysis human human cell hypothesis immunofluorescence incubation time male male fertility male infertility membrane binding membrane fluidity membrane permeability pathogenesis polarization priority journal protein phosphorylation semen analysis sperm donor spermatozoon capacitation spermatozoon count spermatozoon motility velocity Western blotting zona pellucida Adult Analysis of Variance Blotting, Western Bucladesine Case-Control Studies Cells, Cultured Fluorescent Antibody Technique, Indirect Humans Image Processing, Computer-Assisted Male Membrane Fluidity Microscopy, Fluorescence Oligospermia Pentoxifylline Phosphodiesterase Inhibitors Phosphorylation Phosphotyrosine Sperm Capacitation Sperm Motility Spermatozoa Tyrosine Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients |
| topic_facet |
cyclic AMP laurdan phosphodiesterase inhibitor tyrosine adult article asthenospermia cell activation cell isolation cell membrane cell subpopulation clinical article computer analysis controlled study disease association fertilization fluorescence analysis human human cell hypothesis immunofluorescence incubation time male male fertility male infertility membrane binding membrane fluidity membrane permeability pathogenesis polarization priority journal protein phosphorylation semen analysis sperm donor spermatozoon capacitation spermatozoon count spermatozoon motility velocity Western blotting zona pellucida Adult Analysis of Variance Blotting, Western Bucladesine Case-Control Studies Cells, Cultured Fluorescent Antibody Technique, Indirect Humans Image Processing, Computer-Assisted Male Membrane Fluidity Microscopy, Fluorescence Oligospermia Pentoxifylline Phosphodiesterase Inhibitors Phosphorylation Phosphotyrosine Sperm Capacitation Sperm Motility Spermatozoa Tyrosine |
| description |
Sperm protein tyrosine phosphorylation has been associated with capacitation, motility changes, zona binding, and fertilizing ability. We previously demonstrated that gradient-isolated human sperm subpopulations differ in their plasma membrane composition, their ability to phosphorylate proteins in tyrosine residues, and their capacity to undergo hyperactivation. In this study, we have characterized capacitation-associated changes in protein tyrosine phosphorylation and membrane fluidity in spermatozoa of asthenozoospermic and normozoospermic patients consulting for infertility. Semen samples were studied at baseline and after a capacitating incubation with or without the addition of a permeable cAMP analog and a phosphodiesterase inhibitor. Basic sperm and computer-assisted motion parameters, hyperactivation, protein tyrosine phosphorylation (immunofluorescence and Western blot), and membrane fluidity (fluorescent Laurdan probe) were the main study parameters. In comparison with normozoospermic and proven-fertile donor semen, asthenozoospermic samples showed lower motility, velocity, and amplitude of lateral head displacement, both originally and after a 6-h capacitating incubation. Unlike those in normal samples, asthenozoospermic spermatozoa were unable to increase protein tyrosine phosphorylation during capacitation. Such impairment, however, was overcome when they were incubated with a membrane-permeable cAMP analog and a phosphodiesterase inhibitor, indicating a possible membrane defect. Confirming this hypothesis, plasma membranes of asthenozoospermic sperm showed decreased fluidity (increased Laurdan polarization), even after a capacitating incubation. In conclusion, spermatozoa from functional asthenozoospermic samples may owe their poor motility, and their inability to properly capacitate and develop hyperactivation, to an impairment in the tyrosine phosphorylation of critical proteins caused by decreased membrane fluidity. These findings suggest a molecular pathogenetic mechanism for a common seminal pathology associated with male infertility. © 2005 Society for Reproduction and Fertility. |
| title |
Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients |
| title_short |
Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients |
| title_full |
Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients |
| title_fullStr |
Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients |
| title_full_unstemmed |
Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients |
| title_sort |
capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermotozoa of asthenozzospermic patients |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v129_n6_p697_Buffone http://hdl.handle.net/20.500.12110/paper_14701626_v129_n6_p697_Buffone |
| _version_ |
1768544792790171648 |