Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan)
Implantation is a crucial event in human pregnancy. The participation of cytokines in the implantation process has been widely documented, although the role of many of these molecules is still a matter of controversy. In a previous report from our laboratory, we demonstrated that addition of interfe...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v128_n6_p717_Fontana http://hdl.handle.net/20.500.12110/paper_14701626_v128_n6_p717_Fontana |
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paper:paper_14701626_v128_n6_p717_Fontana2023-06-08T16:17:02Z Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) epidermal growth factor receptor epidermal growth factor receptor 4 gamma interferon proteoheparan sulfate adhesion animal tissue article blastocyst controlled study embryo embryo culture embryo development female genital system disease immunocytochemistry immunohistochemistry immunopathology in vitro study inner cell mass morphology mouse nonhuman preimplantation embryo priority journal protein expression protein localization trophoblast Animals Blastocyst Embryonic Development Female Heparan Sulfate Proteoglycans Immunohistochemistry Interferon-gamma, Recombinant Mice Receptor, Epidermal Growth Factor Tissue Culture Techniques Trophoblasts Murinae Implantation is a crucial event in human pregnancy. The participation of cytokines in the implantation process has been widely documented, although the role of many of these molecules is still a matter of controversy. In a previous report from our laboratory, we demonstrated that addition of interferon-γ to the culture medium produces deleterious effects on mouse embryo development. In this study we investigated the effect of this cytokine on outgrowing embryo morphology and on the expression of epidermal growth factor receptors (ErbBs) and heparan sulfate proteoglycan (perlecan) in mouse embryos cultured in vitro. Morphological assessment of inner cell mass and trophoblast development was carried on in-situ fixed and stained outgrowths. Localization of ErbB1, ErbB4 and perlecan on pre- and peri-implantation embryos was investigated by immunocytochemistry. Addition of interferon-γ produced a deleterious effect on both inner cell mass and trophoblast morphology. Immunostaining demonstrated that ErbB1, ErbB4 and perlecan are present on pre-implantation embryos and blastocysts; interferon-γ altered the expression of ErbB4 and Perlecan at the blastocyst stage. We propose that the effects produced by this cytokine could be related to the altered acquisition of adhesion competence and low implantation rates observed in certain reproductive immunological disorders. © 2004 Society for Reproduction and Fertility. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v128_n6_p717_Fontana http://hdl.handle.net/20.500.12110/paper_14701626_v128_n6_p717_Fontana |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
epidermal growth factor receptor epidermal growth factor receptor 4 gamma interferon proteoheparan sulfate adhesion animal tissue article blastocyst controlled study embryo embryo culture embryo development female genital system disease immunocytochemistry immunohistochemistry immunopathology in vitro study inner cell mass morphology mouse nonhuman preimplantation embryo priority journal protein expression protein localization trophoblast Animals Blastocyst Embryonic Development Female Heparan Sulfate Proteoglycans Immunohistochemistry Interferon-gamma, Recombinant Mice Receptor, Epidermal Growth Factor Tissue Culture Techniques Trophoblasts Murinae |
| spellingShingle |
epidermal growth factor receptor epidermal growth factor receptor 4 gamma interferon proteoheparan sulfate adhesion animal tissue article blastocyst controlled study embryo embryo culture embryo development female genital system disease immunocytochemistry immunohistochemistry immunopathology in vitro study inner cell mass morphology mouse nonhuman preimplantation embryo priority journal protein expression protein localization trophoblast Animals Blastocyst Embryonic Development Female Heparan Sulfate Proteoglycans Immunohistochemistry Interferon-gamma, Recombinant Mice Receptor, Epidermal Growth Factor Tissue Culture Techniques Trophoblasts Murinae Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) |
| topic_facet |
epidermal growth factor receptor epidermal growth factor receptor 4 gamma interferon proteoheparan sulfate adhesion animal tissue article blastocyst controlled study embryo embryo culture embryo development female genital system disease immunocytochemistry immunohistochemistry immunopathology in vitro study inner cell mass morphology mouse nonhuman preimplantation embryo priority journal protein expression protein localization trophoblast Animals Blastocyst Embryonic Development Female Heparan Sulfate Proteoglycans Immunohistochemistry Interferon-gamma, Recombinant Mice Receptor, Epidermal Growth Factor Tissue Culture Techniques Trophoblasts Murinae |
| description |
Implantation is a crucial event in human pregnancy. The participation of cytokines in the implantation process has been widely documented, although the role of many of these molecules is still a matter of controversy. In a previous report from our laboratory, we demonstrated that addition of interferon-γ to the culture medium produces deleterious effects on mouse embryo development. In this study we investigated the effect of this cytokine on outgrowing embryo morphology and on the expression of epidermal growth factor receptors (ErbBs) and heparan sulfate proteoglycan (perlecan) in mouse embryos cultured in vitro. Morphological assessment of inner cell mass and trophoblast development was carried on in-situ fixed and stained outgrowths. Localization of ErbB1, ErbB4 and perlecan on pre- and peri-implantation embryos was investigated by immunocytochemistry. Addition of interferon-γ produced a deleterious effect on both inner cell mass and trophoblast morphology. Immunostaining demonstrated that ErbB1, ErbB4 and perlecan are present on pre-implantation embryos and blastocysts; interferon-γ altered the expression of ErbB4 and Perlecan at the blastocyst stage. We propose that the effects produced by this cytokine could be related to the altered acquisition of adhesion competence and low implantation rates observed in certain reproductive immunological disorders. © 2004 Society for Reproduction and Fertility. |
| title |
Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) |
| title_short |
Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) |
| title_full |
Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) |
| title_fullStr |
Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) |
| title_full_unstemmed |
Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan) |
| title_sort |
exogenous interferon-γ alters murine inner cell mass and trophoblast development. effect on the expression of erbb1, erbb4 and heparan sulfate proteoglycan (perlecan) |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v128_n6_p717_Fontana http://hdl.handle.net/20.500.12110/paper_14701626_v128_n6_p717_Fontana |
| _version_ |
1768541813910536192 |