ArcA redox mutants as a source of reduced bioproducts

Escherichia coli and other facultative anaerobes can adapt their metabolism according to oxygen availability by means of aerobic and anaerobic respiration and fermentation. ArcAB is a two-component signal transduction system that controls, at the transcriptional level, the choice of energy generatio...

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Autores principales:	Pettinari, María Julia, Ruiz, Jimena Alicia, Méndez, Beatriz Silvia
Publicado:	2008
Materias:	ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14641801_v15_n1_p41_Pettinari http://hdl.handle.net/20.500.12110/paper_14641801_v15_n1_p41_Pettinari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14641801_v15_n1_p41_Pettinari
record_format	dspace
spelling	paper:paper_14641801_v15_n1_p41_Pettinari2025-07-30T18:52:23Z ArcA redox mutants as a source of reduced bioproducts Pettinari, María Julia Ruiz, Jimena Alicia Méndez, Beatriz Silvia ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli Escherichia coli and other facultative anaerobes can adapt their metabolism according to oxygen availability by means of aerobic and anaerobic respiration and fermentation. ArcAB is a two-component signal transduction system that controls, at the transcriptional level, the choice of energy generation pathway according to the intracellular redox state. High throughput studies on different redox regulator mutants, involving transcriptome analysis, RT-PCR and phenotypic arrays enabled the elucidation of a repertoire of operons coordinated by ArcA which extended beyond respiration control including, among others, those which code for survival, chromosome replication and degradation of fatty acids. Flux analysis by 13C labeling provided new clues to the understanding of the distribution of metabolites mediated by ArcAB. The genetic manipulation of this regulator proved to be useful for the generation of reduced products of commercial value. Copyright © 2008 S. Karger AG. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14641801_v15_n1_p41_Pettinari http://hdl.handle.net/20.500.12110/paper_14641801_v15_n1_p41_Pettinari
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli
spellingShingle	ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli Pettinari, María Julia Ruiz, Jimena Alicia Méndez, Beatriz Silvia ArcA redox mutants as a source of reduced bioproducts
topic_facet	ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli
description	Escherichia coli and other facultative anaerobes can adapt their metabolism according to oxygen availability by means of aerobic and anaerobic respiration and fermentation. ArcAB is a two-component signal transduction system that controls, at the transcriptional level, the choice of energy generation pathway according to the intracellular redox state. High throughput studies on different redox regulator mutants, involving transcriptome analysis, RT-PCR and phenotypic arrays enabled the elucidation of a repertoire of operons coordinated by ArcA which extended beyond respiration control including, among others, those which code for survival, chromosome replication and degradation of fatty acids. Flux analysis by 13C labeling provided new clues to the understanding of the distribution of metabolites mediated by ArcAB. The genetic manipulation of this regulator proved to be useful for the generation of reduced products of commercial value. Copyright © 2008 S. Karger AG.
author	Pettinari, María Julia Ruiz, Jimena Alicia Méndez, Beatriz Silvia
author_facet	Pettinari, María Julia Ruiz, Jimena Alicia Méndez, Beatriz Silvia
author_sort	Pettinari, María Julia
title	ArcA redox mutants as a source of reduced bioproducts
title_short	ArcA redox mutants as a source of reduced bioproducts
title_full	ArcA redox mutants as a source of reduced bioproducts
title_fullStr	ArcA redox mutants as a source of reduced bioproducts
title_full_unstemmed	ArcA redox mutants as a source of reduced bioproducts
title_sort	arca redox mutants as a source of reduced bioproducts
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14641801_v15_n1_p41_Pettinari http://hdl.handle.net/20.500.12110/paper_14641801_v15_n1_p41_Pettinari
work_keys_str_mv	AT pettinarimariajulia arcaredoxmutantsasasourceofreducedbioproducts AT ruizjimenaalicia arcaredoxmutantsasasourceofreducedbioproducts AT mendezbeatrizsilvia arcaredoxmutantsasasourceofreducedbioproducts
_version_	1840327526435520512

ArcA redox mutants as a source of reduced bioproducts

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