Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication
Summary: Translation efficiency of viral mRNAs is a key factor defining both cytopathogenicity and virulence of viruses, which are entirely dependent on the cellular translation machinery to synthesize their proteins. This dependence has led them to develop different translational reprogramming stra...
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paper:paper_14625814_v15_n10_p1766_Linero2023-06-08T16:16:16Z Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication Linero, Florencia N. Scolaro, Luis Alberto 2 morpholino 8 phenylchromone initiation factor 4A initiation factor 4E initiation factor 4F initiation factor 4G messenger RNA rapamycin virus protein amino terminal sequence Argentine hemorrhagic fever article cellular distribution comparative study confocal microscopy controlled study cytoplasm Encephalomyocarditis virus immunoprecipitation Junin virus nonhuman oxidative stress Pichinde arenavirus polyacrylamide gel electrophoresis priority journal protein secondary structure protein synthesis synthesis translation initiation Vero cell virogenesis virus culture virus replication Arenaviridae Junin virus Pichinde virus Animals Cercopithecus aethiops Eukaryotic Initiation Factor-4A Eukaryotic Initiation Factor-4E Eukaryotic Initiation Factor-4G Guanosine Host-Pathogen Interactions Junin virus Nucleocapsid Proteins Vero Cells Virus Replication Summary: Translation efficiency of viral mRNAs is a key factor defining both cytopathogenicity and virulence of viruses, which are entirely dependent on the cellular translation machinery to synthesize their proteins. This dependence has led them to develop different translational reprogramming strategies to ensure viral mRNAs can effectively compete with cellular mRNAs. Junin virus (JUNV) is a member of the family Arenaviridae, whose mRNAs are capped but not polyadenylated. In this work we evaluated the relevance to JUNV replication of the main components of the eIF4F complex: eIF4A, eIF4GI and eIF4E. We found the viral nucleoprotein (N) of JUNV colocalized with eIF4A and eIF4GI but not with eIF4E. Moreover, N could be immunoprecipitated in association with eIF4A and eIF4GI but not with eIF4E. Accordingly, functional impairment of eIF4A as well as eIF4GI reduced JUNV multiplication. By contrast, inhibition of eIF4E did not show a significant effect on JUNV protein synthesis. A similar situation was observed for another two members of arenaviruses: Tacaribe (TCRV) and Pichinde (PICV) viruses. Finally, the nucleoproteins of JUNV, TCRV and PICV were able to interact with 7 methyl-guanosine (cap), suggesting that the independence of JUNV multiplication on eIF4E, the cap-binding protein, may be due to the replacement of this factor by N protein. © 2013 John Wiley & Sons Ltd. Fil:Linero, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14625814_v15_n10_p1766_Linero http://hdl.handle.net/20.500.12110/paper_14625814_v15_n10_p1766_Linero |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
2 morpholino 8 phenylchromone initiation factor 4A initiation factor 4E initiation factor 4F initiation factor 4G messenger RNA rapamycin virus protein amino terminal sequence Argentine hemorrhagic fever article cellular distribution comparative study confocal microscopy controlled study cytoplasm Encephalomyocarditis virus immunoprecipitation Junin virus nonhuman oxidative stress Pichinde arenavirus polyacrylamide gel electrophoresis priority journal protein secondary structure protein synthesis synthesis translation initiation Vero cell virogenesis virus culture virus replication Arenaviridae Junin virus Pichinde virus Animals Cercopithecus aethiops Eukaryotic Initiation Factor-4A Eukaryotic Initiation Factor-4E Eukaryotic Initiation Factor-4G Guanosine Host-Pathogen Interactions Junin virus Nucleocapsid Proteins Vero Cells Virus Replication |
spellingShingle |
2 morpholino 8 phenylchromone initiation factor 4A initiation factor 4E initiation factor 4F initiation factor 4G messenger RNA rapamycin virus protein amino terminal sequence Argentine hemorrhagic fever article cellular distribution comparative study confocal microscopy controlled study cytoplasm Encephalomyocarditis virus immunoprecipitation Junin virus nonhuman oxidative stress Pichinde arenavirus polyacrylamide gel electrophoresis priority journal protein secondary structure protein synthesis synthesis translation initiation Vero cell virogenesis virus culture virus replication Arenaviridae Junin virus Pichinde virus Animals Cercopithecus aethiops Eukaryotic Initiation Factor-4A Eukaryotic Initiation Factor-4E Eukaryotic Initiation Factor-4G Guanosine Host-Pathogen Interactions Junin virus Nucleocapsid Proteins Vero Cells Virus Replication Linero, Florencia N. Scolaro, Luis Alberto Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication |
topic_facet |
2 morpholino 8 phenylchromone initiation factor 4A initiation factor 4E initiation factor 4F initiation factor 4G messenger RNA rapamycin virus protein amino terminal sequence Argentine hemorrhagic fever article cellular distribution comparative study confocal microscopy controlled study cytoplasm Encephalomyocarditis virus immunoprecipitation Junin virus nonhuman oxidative stress Pichinde arenavirus polyacrylamide gel electrophoresis priority journal protein secondary structure protein synthesis synthesis translation initiation Vero cell virogenesis virus culture virus replication Arenaviridae Junin virus Pichinde virus Animals Cercopithecus aethiops Eukaryotic Initiation Factor-4A Eukaryotic Initiation Factor-4E Eukaryotic Initiation Factor-4G Guanosine Host-Pathogen Interactions Junin virus Nucleocapsid Proteins Vero Cells Virus Replication |
description |
Summary: Translation efficiency of viral mRNAs is a key factor defining both cytopathogenicity and virulence of viruses, which are entirely dependent on the cellular translation machinery to synthesize their proteins. This dependence has led them to develop different translational reprogramming strategies to ensure viral mRNAs can effectively compete with cellular mRNAs. Junin virus (JUNV) is a member of the family Arenaviridae, whose mRNAs are capped but not polyadenylated. In this work we evaluated the relevance to JUNV replication of the main components of the eIF4F complex: eIF4A, eIF4GI and eIF4E. We found the viral nucleoprotein (N) of JUNV colocalized with eIF4A and eIF4GI but not with eIF4E. Moreover, N could be immunoprecipitated in association with eIF4A and eIF4GI but not with eIF4E. Accordingly, functional impairment of eIF4A as well as eIF4GI reduced JUNV multiplication. By contrast, inhibition of eIF4E did not show a significant effect on JUNV protein synthesis. A similar situation was observed for another two members of arenaviruses: Tacaribe (TCRV) and Pichinde (PICV) viruses. Finally, the nucleoproteins of JUNV, TCRV and PICV were able to interact with 7 methyl-guanosine (cap), suggesting that the independence of JUNV multiplication on eIF4E, the cap-binding protein, may be due to the replacement of this factor by N protein. © 2013 John Wiley & Sons Ltd. |
author |
Linero, Florencia N. Scolaro, Luis Alberto |
author_facet |
Linero, Florencia N. Scolaro, Luis Alberto |
author_sort |
Linero, Florencia N. |
title |
Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication |
title_short |
Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication |
title_full |
Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication |
title_fullStr |
Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication |
title_full_unstemmed |
Participation Of Eif4F Complex In Junin Virus Infection: Blockage Of Eif4E Does Not Impair Virus Replication |
title_sort |
participation of eif4f complex in junin virus infection: blockage of eif4e does not impair virus replication |
publishDate |
2013 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14625814_v15_n10_p1766_Linero http://hdl.handle.net/20.500.12110/paper_14625814_v15_n10_p1766_Linero |
work_keys_str_mv |
AT lineroflorencian participationofeif4fcomplexinjuninvirusinfectionblockageofeif4edoesnotimpairvirusreplication AT scolaroluisalberto participationofeif4fcomplexinjuninvirusinfectionblockageofeif4edoesnotimpairvirusreplication |
_version_ |
1768542379493556224 |