Alternative infectious entry pathways for dengue virus serotypes into mammalian cells
The entry of two dengue virus (DENV) serotypes into Vero cells was analysed using biochemical inhibitors, dominant negative mutants of cellular proteins involved in endocytic pathways, fluorescence microscopy and infectivity determinations. By treatment with dansylcadaverine and chlorpromazine and o...
| Autores principales: | , , |
|---|---|
| Publicado: |
2009
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14625814_v11_n10_p1533_Acosta http://hdl.handle.net/20.500.12110/paper_14625814_v11_n10_p1533_Acosta |
| Aporte de: |
| id |
paper:paper_14625814_v11_n10_p1533_Acosta |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_14625814_v11_n10_p1533_Acosta2023-06-08T16:16:15Z Alternative infectious entry pathways for dengue virus serotypes into mammalian cells Acosta, Eliana Gisela Castilla, Viviana Damonte, Elsa Beatriz clathrin dynamin epidermal growth factor receptor pathway substrate 15 clathrin dynamin animal cell article cell invasion controlled study dengue Dengue virus endocytosis human human cell internalization nonhuman priority journal protein function serotype Vero cell virus entry animal cell line Cercopithecus Dengue virus metabolism pathogenicity physiology Dengue virus Mammalia Animals Cell Line Cercopithecus aethiops Clathrin Dengue Virus Dynamins Endocytosis Humans Virus Internalization The entry of two dengue virus (DENV) serotypes into Vero cells was analysed using biochemical inhibitors, dominant negative mutants of cellular proteins involved in endocytic pathways, fluorescence microscopy and infectivity determinations. By treatment with dansylcadaverine and chlorpromazine and overexpression of a dominant negative form of the Eps15 protein, a clathrin-mediated endocytosis for productive DENV-1 internalization into Vero cells was demonstrated whereas the infectious entry of DENV-2 in the same cell system was independent of clathrin. Treatment with the inhibitors nystatin and methyl-β-cyclodextrin, as well as transfection of Vero cells with dominant negative caveolin-1, had no effect on DENV-2 virus infection. It was also shown, by using the K44A mutant and the inhibitor dynasore, that dynamin was required for DENV-2 entry. Consequently, the infectious entry of DENV-2 into Vero cells occurs by a non-classical endocytic pathway independent of clathrin, caveolae and lipid rafts, but dependent on dynamin. By contrast, DENV-2 entry into A549 cells was clathrin-dependent, as previously reported in HeLa, C6/36 and BS-C-1 cells. Our results conclusively show, for the first time, a differential mode of infective entry for DENV-1 and DENV-2 into a common host cell, Vero cells, as well as alternative entry pathways for a given serotype, DENV-2, into different types of cells. © 2009 Blackwell Publishing Ltd. Fil:Acosta, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14625814_v11_n10_p1533_Acosta http://hdl.handle.net/20.500.12110/paper_14625814_v11_n10_p1533_Acosta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
clathrin dynamin epidermal growth factor receptor pathway substrate 15 clathrin dynamin animal cell article cell invasion controlled study dengue Dengue virus endocytosis human human cell internalization nonhuman priority journal protein function serotype Vero cell virus entry animal cell line Cercopithecus Dengue virus metabolism pathogenicity physiology Dengue virus Mammalia Animals Cell Line Cercopithecus aethiops Clathrin Dengue Virus Dynamins Endocytosis Humans Virus Internalization |
| spellingShingle |
clathrin dynamin epidermal growth factor receptor pathway substrate 15 clathrin dynamin animal cell article cell invasion controlled study dengue Dengue virus endocytosis human human cell internalization nonhuman priority journal protein function serotype Vero cell virus entry animal cell line Cercopithecus Dengue virus metabolism pathogenicity physiology Dengue virus Mammalia Animals Cell Line Cercopithecus aethiops Clathrin Dengue Virus Dynamins Endocytosis Humans Virus Internalization Acosta, Eliana Gisela Castilla, Viviana Damonte, Elsa Beatriz Alternative infectious entry pathways for dengue virus serotypes into mammalian cells |
| topic_facet |
clathrin dynamin epidermal growth factor receptor pathway substrate 15 clathrin dynamin animal cell article cell invasion controlled study dengue Dengue virus endocytosis human human cell internalization nonhuman priority journal protein function serotype Vero cell virus entry animal cell line Cercopithecus Dengue virus metabolism pathogenicity physiology Dengue virus Mammalia Animals Cell Line Cercopithecus aethiops Clathrin Dengue Virus Dynamins Endocytosis Humans Virus Internalization |
| description |
The entry of two dengue virus (DENV) serotypes into Vero cells was analysed using biochemical inhibitors, dominant negative mutants of cellular proteins involved in endocytic pathways, fluorescence microscopy and infectivity determinations. By treatment with dansylcadaverine and chlorpromazine and overexpression of a dominant negative form of the Eps15 protein, a clathrin-mediated endocytosis for productive DENV-1 internalization into Vero cells was demonstrated whereas the infectious entry of DENV-2 in the same cell system was independent of clathrin. Treatment with the inhibitors nystatin and methyl-β-cyclodextrin, as well as transfection of Vero cells with dominant negative caveolin-1, had no effect on DENV-2 virus infection. It was also shown, by using the K44A mutant and the inhibitor dynasore, that dynamin was required for DENV-2 entry. Consequently, the infectious entry of DENV-2 into Vero cells occurs by a non-classical endocytic pathway independent of clathrin, caveolae and lipid rafts, but dependent on dynamin. By contrast, DENV-2 entry into A549 cells was clathrin-dependent, as previously reported in HeLa, C6/36 and BS-C-1 cells. Our results conclusively show, for the first time, a differential mode of infective entry for DENV-1 and DENV-2 into a common host cell, Vero cells, as well as alternative entry pathways for a given serotype, DENV-2, into different types of cells. © 2009 Blackwell Publishing Ltd. |
| author |
Acosta, Eliana Gisela Castilla, Viviana Damonte, Elsa Beatriz |
| author_facet |
Acosta, Eliana Gisela Castilla, Viviana Damonte, Elsa Beatriz |
| author_sort |
Acosta, Eliana Gisela |
| title |
Alternative infectious entry pathways for dengue virus serotypes into mammalian cells |
| title_short |
Alternative infectious entry pathways for dengue virus serotypes into mammalian cells |
| title_full |
Alternative infectious entry pathways for dengue virus serotypes into mammalian cells |
| title_fullStr |
Alternative infectious entry pathways for dengue virus serotypes into mammalian cells |
| title_full_unstemmed |
Alternative infectious entry pathways for dengue virus serotypes into mammalian cells |
| title_sort |
alternative infectious entry pathways for dengue virus serotypes into mammalian cells |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14625814_v11_n10_p1533_Acosta http://hdl.handle.net/20.500.12110/paper_14625814_v11_n10_p1533_Acosta |
| work_keys_str_mv |
AT acostaelianagisela alternativeinfectiousentrypathwaysfordenguevirusserotypesintomammaliancells AT castillaviviana alternativeinfectiousentrypathwaysfordenguevirusserotypesintomammaliancells AT damonteelsabeatriz alternativeinfectiousentrypathwaysfordenguevirusserotypesintomammaliancells |
| _version_ |
1768544381539713024 |