Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic
Summary: The diversity of naphthalene dioxygenase genes (ndo) in soil environments from the Maritime Antarctic was assessed, dissecting as well the influence of the two vascular plants that grow in the Antarctic: Deschampsia antarctica and Colobanthus quitensis. Total community DNA was extracted fro...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14622912_v11_n3_p700_Flocco http://hdl.handle.net/20.500.12110/paper_14622912_v11_n3_p700_Flocco |
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paper:paper_14622912_v11_n3_p700_Flocco2023-06-08T16:16:13Z Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic bacterial DNA multienzyme complex naphthalene dioxygenase oxygenase Antarctica article chemistry DNA fingerprinting DNA sequence genetic variability genetics microbiology molecular genetics nucleotide sequence phylogeny polymerase chain reaction Pseudomonas sequence homology Antarctic Regions DNA Fingerprinting DNA, Bacterial Genetic Variation Molecular Sequence Data Multienzyme Complexes Oxygenases Phylogeny Polymerase Chain Reaction Pseudomonas Sequence Analysis, DNA Sequence Homology Soil Microbiology Colobanthus quitensis Deschampsia antarctica Pseudomonas Tracheophyta Summary: The diversity of naphthalene dioxygenase genes (ndo) in soil environments from the Maritime Antarctic was assessed, dissecting as well the influence of the two vascular plants that grow in the Antarctic: Deschampsia antarctica and Colobanthus quitensis. Total community DNA was extracted from bulk and rhizosphere soil samples from Jubany station and Potter Peninsula, South Shetland Islands. ndo genes were amplified by a nested PCR and analysed by denaturant gradient gel electrophoresis approach (PCR-DGGE) and cloning and sequencing. The ndo-DGGE fingerprints of oil-contaminated soil samples showed even and reproducible patterns, composed of four dominant bands. The presence of vascular plants did not change the relative abundance of ndo genotypes compared with bulk soil. For non-contaminated sites, amplicons were not obtained for all replicates and the variability among the fingerprints was comparatively higher, likely reflecting a lower abundance of ndo genes. The phylogenetic analyses showed that all sequences were affiliated to the nahAc genes closely related to those described for Pseudomonas species and related mobile genetic elements. This study revealed that a microdiversity of nahAc-like genes exists in microbial communities of Antarctic soils and quantitative PCR indicated that their relative abundance was increased in response to anthropogenic sources of pollution. © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14622912_v11_n3_p700_Flocco http://hdl.handle.net/20.500.12110/paper_14622912_v11_n3_p700_Flocco |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial DNA multienzyme complex naphthalene dioxygenase oxygenase Antarctica article chemistry DNA fingerprinting DNA sequence genetic variability genetics microbiology molecular genetics nucleotide sequence phylogeny polymerase chain reaction Pseudomonas sequence homology Antarctic Regions DNA Fingerprinting DNA, Bacterial Genetic Variation Molecular Sequence Data Multienzyme Complexes Oxygenases Phylogeny Polymerase Chain Reaction Pseudomonas Sequence Analysis, DNA Sequence Homology Soil Microbiology Colobanthus quitensis Deschampsia antarctica Pseudomonas Tracheophyta |
| spellingShingle |
bacterial DNA multienzyme complex naphthalene dioxygenase oxygenase Antarctica article chemistry DNA fingerprinting DNA sequence genetic variability genetics microbiology molecular genetics nucleotide sequence phylogeny polymerase chain reaction Pseudomonas sequence homology Antarctic Regions DNA Fingerprinting DNA, Bacterial Genetic Variation Molecular Sequence Data Multienzyme Complexes Oxygenases Phylogeny Polymerase Chain Reaction Pseudomonas Sequence Analysis, DNA Sequence Homology Soil Microbiology Colobanthus quitensis Deschampsia antarctica Pseudomonas Tracheophyta Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic |
| topic_facet |
bacterial DNA multienzyme complex naphthalene dioxygenase oxygenase Antarctica article chemistry DNA fingerprinting DNA sequence genetic variability genetics microbiology molecular genetics nucleotide sequence phylogeny polymerase chain reaction Pseudomonas sequence homology Antarctic Regions DNA Fingerprinting DNA, Bacterial Genetic Variation Molecular Sequence Data Multienzyme Complexes Oxygenases Phylogeny Polymerase Chain Reaction Pseudomonas Sequence Analysis, DNA Sequence Homology Soil Microbiology Colobanthus quitensis Deschampsia antarctica Pseudomonas Tracheophyta |
| description |
Summary: The diversity of naphthalene dioxygenase genes (ndo) in soil environments from the Maritime Antarctic was assessed, dissecting as well the influence of the two vascular plants that grow in the Antarctic: Deschampsia antarctica and Colobanthus quitensis. Total community DNA was extracted from bulk and rhizosphere soil samples from Jubany station and Potter Peninsula, South Shetland Islands. ndo genes were amplified by a nested PCR and analysed by denaturant gradient gel electrophoresis approach (PCR-DGGE) and cloning and sequencing. The ndo-DGGE fingerprints of oil-contaminated soil samples showed even and reproducible patterns, composed of four dominant bands. The presence of vascular plants did not change the relative abundance of ndo genotypes compared with bulk soil. For non-contaminated sites, amplicons were not obtained for all replicates and the variability among the fingerprints was comparatively higher, likely reflecting a lower abundance of ndo genes. The phylogenetic analyses showed that all sequences were affiliated to the nahAc genes closely related to those described for Pseudomonas species and related mobile genetic elements. This study revealed that a microdiversity of nahAc-like genes exists in microbial communities of Antarctic soils and quantitative PCR indicated that their relative abundance was increased in response to anthropogenic sources of pollution. © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd. |
| title |
Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic |
| title_short |
Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic |
| title_full |
Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic |
| title_fullStr |
Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic |
| title_full_unstemmed |
Occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the Maritime Antarctic |
| title_sort |
occurrence and diversity of naphthalene dioxygenase genes in soil microbial communities from the maritime antarctic |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14622912_v11_n3_p700_Flocco http://hdl.handle.net/20.500.12110/paper_14622912_v11_n3_p700_Flocco |
| _version_ |
1768541908564443136 |