The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses

We describe for the first time the karyotype of the black howler monkey, Alouatta pigra. Conventional staining, G- and C-banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were performed. Eight free ranging adult individuals, four males and fo...

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Detalles Bibliográficos
Publicado:	2008
Materias:	peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Primates
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v122_n2_p103_Steinberg http://hdl.handle.net/20.500.12110/paper_14248581_v122_n2_p103_Steinberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14248581_v122_n2_p103_Steinberg
record_format	dspace
spelling	paper:paper_14248581_v122_n2_p103_Steinberg2023-06-08T16:14:01Z The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates We describe for the first time the karyotype of the black howler monkey, Alouatta pigra. Conventional staining, G- and C-banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were performed. Eight free ranging adult individuals, four males and four females, within the natural distribution of the species presented a diploid karyotype with 2n = 58. Mitotic analyses showed an autosomal complement composed of 6 submetacentric, 3 metacentric, and 19 acrocentric chromosome pairs for females, and 6 submetacentric, 3 metacentric, and 18 acrocentric pairs for males. Meiotic analyses in males revealed 27 autosomal bivalents and a quadrivalent composed of a submetacentric X1 and acrocentric X 2, Y1, and Y2. The G-banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the quadrivalent components. C-banding technique in metaphase I corroborated the structure of the quadrivalent showing four C+ centromeres. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. Copyright © 2008 S. Karger AG. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v122_n2_p103_Steinberg http://hdl.handle.net/20.500.12110/paper_14248581_v122_n2_p103_Steinberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates
spellingShingle	peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses
topic_facet	peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates
description	We describe for the first time the karyotype of the black howler monkey, Alouatta pigra. Conventional staining, G- and C-banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were performed. Eight free ranging adult individuals, four males and four females, within the natural distribution of the species presented a diploid karyotype with 2n = 58. Mitotic analyses showed an autosomal complement composed of 6 submetacentric, 3 metacentric, and 19 acrocentric chromosome pairs for females, and 6 submetacentric, 3 metacentric, and 18 acrocentric pairs for males. Meiotic analyses in males revealed 27 autosomal bivalents and a quadrivalent composed of a submetacentric X1 and acrocentric X 2, Y1, and Y2. The G-banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the quadrivalent components. C-banding technique in metaphase I corroborated the structure of the quadrivalent showing four C+ centromeres. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. Copyright © 2008 S. Karger AG.
title	The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses
title_short	The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses
title_full	The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses
title_fullStr	The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses
title_full_unstemmed	The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses
title_sort	karyotype of alouattapigra (primates: platyrrhini): mitotic and meiotic analyses
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v122_n2_p103_Steinberg http://hdl.handle.net/20.500.12110/paper_14248581_v122_n2_p103_Steinberg
_version_	1768545524958363648

The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses

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