Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges
Sentinel species are useful tools for studying the deleterious effects of xenobiotics on wildlife. The large hairy armadillo (Chaetophractus villosus) is the most abundant and widely distributed mammal in Argentina. It is a long-lived, omnivorous, burrowing species, with fairly restricted home range...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v796_n_p40_Rossi http://hdl.handle.net/20.500.12110/paper_13835718_v796_n_p40_Rossi |
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paper:paper_13835718_v796_n_p40_Rossi2023-06-08T16:12:31Z Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges Biomarkers Chaetophractus villosus Chromosome aberrations Genotoxicity Sentinel organism Sister chromatid exchange biological marker mitomycin xenobiotic agent mitomycin xenobiotic agent adult animal cell armadillo Article autosome biological monitoring cell proliferation Chaetophractus villosus chromosome aberration controlled study DNA damage female genetic damage genotoxicity karyotype lymphocyte culture male mitosis index nonhuman priority journal sex chromosome sister chromatid sister chromatid exchange animal chemically induced chromosome aberration drug effects lymphocyte metabolism Xenarthra Animals Chromosome Aberrations DNA Damage Female Lymphocytes Male Mitomycin Mitotic Index Sister Chromatid Exchange Xenarthra Xenobiotics Sentinel species are useful tools for studying the deleterious effects of xenobiotics on wildlife. The large hairy armadillo (Chaetophractus villosus) is the most abundant and widely distributed mammal in Argentina. It is a long-lived, omnivorous, burrowing species, with fairly restricted home ranges. To evaluate the level of spontaneous genetic damage in this mammal, we determined the baseline values of several genotoxicity biomarkers. The study included 20 C. villosus adults of both sexes from eight pristine localities within its geographic distribution range. Genotoxicity analysis was performed on 72-h lymphocyte cultures, using mitomycin C as positive control. We obtained the baseline values of mitotic index (MI = 10.52 ± 0.30 metaphases/total cells, n = 20), chromosome aberrations (CA = 0.13 ± 0.22, n = 20), sister chromatid exchanges (SCE) = 6.55 ± 0.26, n = 6) and replication index (RI = 1.66, n = 6). MI and CA did not show significant differences (P > 0.05) among localities or between sexes. No significant differences in MI, CA, SCE, and RI (P > 0.05) were found between values from the pristine localities and historical data. There were significant differences in CA, SCE, and RI (P < 0.05) between lymphocyte cultures from pristine localities and those exposed to mitomycin C. We propose the large hairy armadillo as a sentinel organism for environmental biomonitoring of genotoxic chemicals due to its abundance, easy manipulation, well-known biology, the fact that it is usually exposed to different mixtures and concentrations of environmental contaminants, and the baseline values of genetic damage characterized by MI, CA, SCE and RI as biomarkers. © 2015 Elsevier B.V. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v796_n_p40_Rossi http://hdl.handle.net/20.500.12110/paper_13835718_v796_n_p40_Rossi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biomarkers Chaetophractus villosus Chromosome aberrations Genotoxicity Sentinel organism Sister chromatid exchange biological marker mitomycin xenobiotic agent mitomycin xenobiotic agent adult animal cell armadillo Article autosome biological monitoring cell proliferation Chaetophractus villosus chromosome aberration controlled study DNA damage female genetic damage genotoxicity karyotype lymphocyte culture male mitosis index nonhuman priority journal sex chromosome sister chromatid sister chromatid exchange animal chemically induced chromosome aberration drug effects lymphocyte metabolism Xenarthra Animals Chromosome Aberrations DNA Damage Female Lymphocytes Male Mitomycin Mitotic Index Sister Chromatid Exchange Xenarthra Xenobiotics |
| spellingShingle |
Biomarkers Chaetophractus villosus Chromosome aberrations Genotoxicity Sentinel organism Sister chromatid exchange biological marker mitomycin xenobiotic agent mitomycin xenobiotic agent adult animal cell armadillo Article autosome biological monitoring cell proliferation Chaetophractus villosus chromosome aberration controlled study DNA damage female genetic damage genotoxicity karyotype lymphocyte culture male mitosis index nonhuman priority journal sex chromosome sister chromatid sister chromatid exchange animal chemically induced chromosome aberration drug effects lymphocyte metabolism Xenarthra Animals Chromosome Aberrations DNA Damage Female Lymphocytes Male Mitomycin Mitotic Index Sister Chromatid Exchange Xenarthra Xenobiotics Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges |
| topic_facet |
Biomarkers Chaetophractus villosus Chromosome aberrations Genotoxicity Sentinel organism Sister chromatid exchange biological marker mitomycin xenobiotic agent mitomycin xenobiotic agent adult animal cell armadillo Article autosome biological monitoring cell proliferation Chaetophractus villosus chromosome aberration controlled study DNA damage female genetic damage genotoxicity karyotype lymphocyte culture male mitosis index nonhuman priority journal sex chromosome sister chromatid sister chromatid exchange animal chemically induced chromosome aberration drug effects lymphocyte metabolism Xenarthra Animals Chromosome Aberrations DNA Damage Female Lymphocytes Male Mitomycin Mitotic Index Sister Chromatid Exchange Xenarthra Xenobiotics |
| description |
Sentinel species are useful tools for studying the deleterious effects of xenobiotics on wildlife. The large hairy armadillo (Chaetophractus villosus) is the most abundant and widely distributed mammal in Argentina. It is a long-lived, omnivorous, burrowing species, with fairly restricted home ranges. To evaluate the level of spontaneous genetic damage in this mammal, we determined the baseline values of several genotoxicity biomarkers. The study included 20 C. villosus adults of both sexes from eight pristine localities within its geographic distribution range. Genotoxicity analysis was performed on 72-h lymphocyte cultures, using mitomycin C as positive control. We obtained the baseline values of mitotic index (MI = 10.52 ± 0.30 metaphases/total cells, n = 20), chromosome aberrations (CA = 0.13 ± 0.22, n = 20), sister chromatid exchanges (SCE) = 6.55 ± 0.26, n = 6) and replication index (RI = 1.66, n = 6). MI and CA did not show significant differences (P > 0.05) among localities or between sexes. No significant differences in MI, CA, SCE, and RI (P > 0.05) were found between values from the pristine localities and historical data. There were significant differences in CA, SCE, and RI (P < 0.05) between lymphocyte cultures from pristine localities and those exposed to mitomycin C. We propose the large hairy armadillo as a sentinel organism for environmental biomonitoring of genotoxic chemicals due to its abundance, easy manipulation, well-known biology, the fact that it is usually exposed to different mixtures and concentrations of environmental contaminants, and the baseline values of genetic damage characterized by MI, CA, SCE and RI as biomarkers. © 2015 Elsevier B.V. |
| title |
Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges |
| title_short |
Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges |
| title_full |
Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges |
| title_fullStr |
Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges |
| title_full_unstemmed |
Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges |
| title_sort |
chaetophractus villosus as a sentinel organism: baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v796_n_p40_Rossi http://hdl.handle.net/20.500.12110/paper_13835718_v796_n_p40_Rossi |
| _version_ |
1768544097716404224 |