Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test

The genotoxicity of pesticides is an issue of worldwide concern. The present study was undertaken to evaluate the genotoxic potential of a widely used herbicide formulation, Roundup® (glyphosate), in erythrocytes of broad-snouted caiman (Caiman latirostris) after in ovo exposure. Caiman embryos were...

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Autores principales:	Poletta, Gisela Laura, Mudry, Marta Dolores
Publicado:	2009
Materias:	Caiman latirostris Comet assay Glyphosate formulations Micronucleous test Pesticides DNA glyphosate alligator animal cell article blood sampling caiman latirostris comet assay controlled study DNA damage embryo erythrocyte genotoxicity hatching micronucleus test nonhuman priority journal Alligators and Crocodiles Animals Comet Assay DNA Damage Erythrocytes Glycine Herbicides Micronucleus Tests Animalia Caiman Spondias purpurea
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v672_n2_p95_Poletta http://hdl.handle.net/20.500.12110/paper_13835718_v672_n2_p95_Poletta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13835718_v672_n2_p95_Poletta
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spelling	paper:paper_13835718_v672_n2_p95_Poletta2023-06-08T16:12:28Z Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test Poletta, Gisela Laura Mudry, Marta Dolores Caiman latirostris Comet assay Glyphosate formulations Micronucleous test Pesticides DNA glyphosate alligator animal cell article blood sampling caiman latirostris comet assay controlled study DNA damage embryo erythrocyte genotoxicity hatching micronucleus test nonhuman priority journal Alligators and Crocodiles Animals Comet Assay DNA Damage Erythrocytes Glycine Herbicides Micronucleus Tests Animalia Caiman Caiman latirostris Spondias purpurea The genotoxicity of pesticides is an issue of worldwide concern. The present study was undertaken to evaluate the genotoxic potential of a widely used herbicide formulation, Roundup® (glyphosate), in erythrocytes of broad-snouted caiman (Caiman latirostris) after in ovo exposure. Caiman embryos were exposed at early embryonic stage to different sub-lethal concentrations of Roundup® (50, 100, 200, 300, 400, 500, 750, 1000, 1250 and 1750 μg/egg). At time of hatching, blood samples were obtained from each animal and two short-term tests, the Comet assay and the Micronucleus (MN) test, were performed on erythrocytes to assess DNA damage. A significant increase in DNA damage was observed at a concentration of 500 μg/egg or higher, compared to untreated control animals (p < 0.05). Results from both the Comet assay and the MN test revealed a concentration-dependent effect. This study demonstrated adverse effects of Roundup® on DNA of C. latirostris and confirmed that the Comet assay and the MN test applied on caiman erythrocytes are useful tools in determining potential genotoxicity of pesticides. The identification of sentinel species as well as sensitive biomarkers among the natural biota is imperative to thoroughly evaluate genetic damage, which has significant consequences for short- and long-term survival of the natural species. © 2008 Elsevier B.V. All rights reserved. Fil:Poletta, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v672_n2_p95_Poletta http://hdl.handle.net/20.500.12110/paper_13835718_v672_n2_p95_Poletta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Caiman latirostris Comet assay Glyphosate formulations Micronucleous test Pesticides DNA glyphosate alligator animal cell article blood sampling caiman latirostris comet assay controlled study DNA damage embryo erythrocyte genotoxicity hatching micronucleus test nonhuman priority journal Alligators and Crocodiles Animals Comet Assay DNA Damage Erythrocytes Glycine Herbicides Micronucleus Tests Animalia Caiman Caiman latirostris Spondias purpurea
spellingShingle	Caiman latirostris Comet assay Glyphosate formulations Micronucleous test Pesticides DNA glyphosate alligator animal cell article blood sampling caiman latirostris comet assay controlled study DNA damage embryo erythrocyte genotoxicity hatching micronucleus test nonhuman priority journal Alligators and Crocodiles Animals Comet Assay DNA Damage Erythrocytes Glycine Herbicides Micronucleus Tests Animalia Caiman Caiman latirostris Spondias purpurea Poletta, Gisela Laura Mudry, Marta Dolores Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test
topic_facet	Caiman latirostris Comet assay Glyphosate formulations Micronucleous test Pesticides DNA glyphosate alligator animal cell article blood sampling caiman latirostris comet assay controlled study DNA damage embryo erythrocyte genotoxicity hatching micronucleus test nonhuman priority journal Alligators and Crocodiles Animals Comet Assay DNA Damage Erythrocytes Glycine Herbicides Micronucleus Tests Animalia Caiman Caiman latirostris Spondias purpurea
description	The genotoxicity of pesticides is an issue of worldwide concern. The present study was undertaken to evaluate the genotoxic potential of a widely used herbicide formulation, Roundup® (glyphosate), in erythrocytes of broad-snouted caiman (Caiman latirostris) after in ovo exposure. Caiman embryos were exposed at early embryonic stage to different sub-lethal concentrations of Roundup® (50, 100, 200, 300, 400, 500, 750, 1000, 1250 and 1750 μg/egg). At time of hatching, blood samples were obtained from each animal and two short-term tests, the Comet assay and the Micronucleus (MN) test, were performed on erythrocytes to assess DNA damage. A significant increase in DNA damage was observed at a concentration of 500 μg/egg or higher, compared to untreated control animals (p < 0.05). Results from both the Comet assay and the MN test revealed a concentration-dependent effect. This study demonstrated adverse effects of Roundup® on DNA of C. latirostris and confirmed that the Comet assay and the MN test applied on caiman erythrocytes are useful tools in determining potential genotoxicity of pesticides. The identification of sentinel species as well as sensitive biomarkers among the natural biota is imperative to thoroughly evaluate genetic damage, which has significant consequences for short- and long-term survival of the natural species. © 2008 Elsevier B.V. All rights reserved.
author	Poletta, Gisela Laura Mudry, Marta Dolores
author_facet	Poletta, Gisela Laura Mudry, Marta Dolores
author_sort	Poletta, Gisela Laura
title	Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test
title_short	Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test
title_full	Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test
title_fullStr	Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test
title_full_unstemmed	Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test
title_sort	genotoxicity of the herbicide formulation roundup® (glyphosate) in broad-snouted caiman (caiman latirostris) evidenced by the comet assay and the micronucleus test
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v672_n2_p95_Poletta http://hdl.handle.net/20.500.12110/paper_13835718_v672_n2_p95_Poletta
work_keys_str_mv	AT polettagiselalaura genotoxicityoftheherbicideformulationroundupglyphosateinbroadsnoutedcaimancaimanlatirostrisevidencedbythecometassayandthemicronucleustest AT mudrymartadolores genotoxicityoftheherbicideformulationroundupglyphosateinbroadsnoutedcaimancaimanlatirostrisevidencedbythecometassayandthemicronucleustest
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Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test

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