Cell wall modifications during osmotic stress in Lactobacillus casei

Aims: To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. Methods and Results: Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l -1 NaCl; N condition) and control (MRS; C condition) conditions were deter...

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Autores principales:	Piuri, Mariana, Ruzal, Sandra Mónica
Publicado:	2005
Materias:	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Microscopy, Electron Osmosis Penicillin-Binding Proteins Bacteria (microorganisms) Lactobacillus Posibacteria
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v98_n1_p84_Piuri http://hdl.handle.net/20.500.12110/paper_13645072_v98_n1_p84_Piuri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13645072_v98_n1_p84_Piuri
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spelling	paper:paper_13645072_v98_n1_p84_Piuri2023-06-08T16:11:47Z Cell wall modifications during osmotic stress in Lactobacillus casei Piuri, Mariana Ruzal, Sandra Mónica Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria Aims: To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. Methods and Results: Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l -1 NaCl; N condition) and control (MRS; C condition) conditions were determined by transmission electronic microscopy and analytical procedures. Lactobacillus casei cells grown in N condition were significantly larger than cells grown under unstressed C condition. Increased sensitivity to mutanolysin and antibiotics with target in the cell wall was observed in N condition. Purified cell wall also showed the increased sensitivity to lysis by mutanolysin. Analysis of peptidoglycan (PG) from stressed cells showed that modification was at the structural level in accordance with a decreased PG cross-link involving penicillin-binding proteins (PBP). Nine PBP were first described in this species and these proteins were expressed in low percentages or presented a modified pattern of saturation with penicillin G (Pen G) during growth in high salt. Three of the essential PBP were fully saturated in N condition at lower Pen G concentrations than in C condition, suggesting differences in functionality in vivo. Conclusions: The results show that growth in high salt modified the structural properties of the cell wall. Significance and Impact of Study: Advances in understanding the adaptation to high osmolarity, in particular those involving sensitivity to lysis of lactic acid bacteria. Fil:Piuri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v98_n1_p84_Piuri http://hdl.handle.net/20.500.12110/paper_13645072_v98_n1_p84_Piuri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria
spellingShingle	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria Piuri, Mariana Ruzal, Sandra Mónica Cell wall modifications during osmotic stress in Lactobacillus casei
topic_facet	Lactobacillus casei Osmotic stress PBP Peptidoglycan ampicillin antibiotic agent bacitracin enzyme fosfomycin mutanolysin nisin penicillin binding protein penicillin G peptidoglycan sodium chloride unclassified drug vancomycin cytology microbiology osmoregulation stress analytic method antibiotic sensitivity article bacterial cell bacterial cell wall cell growth controlled study cytolysis in vivo study lactic acid bacterium Lactobacillus casei lysis nonhuman osmolarity osmotic stress purification sensitivity analysis transmission electron microscopy Bacteriological Techniques Cell Wall Drug Resistance Electrophoresis, Polyacrylamide Gel Hydrolysis Lactobacillus casei Microscopy, Electron Osmosis Penicillin-Binding Proteins Peptidoglycan Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria
description	Aims: To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. Methods and Results: Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l -1 NaCl; N condition) and control (MRS; C condition) conditions were determined by transmission electronic microscopy and analytical procedures. Lactobacillus casei cells grown in N condition were significantly larger than cells grown under unstressed C condition. Increased sensitivity to mutanolysin and antibiotics with target in the cell wall was observed in N condition. Purified cell wall also showed the increased sensitivity to lysis by mutanolysin. Analysis of peptidoglycan (PG) from stressed cells showed that modification was at the structural level in accordance with a decreased PG cross-link involving penicillin-binding proteins (PBP). Nine PBP were first described in this species and these proteins were expressed in low percentages or presented a modified pattern of saturation with penicillin G (Pen G) during growth in high salt. Three of the essential PBP were fully saturated in N condition at lower Pen G concentrations than in C condition, suggesting differences in functionality in vivo. Conclusions: The results show that growth in high salt modified the structural properties of the cell wall. Significance and Impact of Study: Advances in understanding the adaptation to high osmolarity, in particular those involving sensitivity to lysis of lactic acid bacteria.
author	Piuri, Mariana Ruzal, Sandra Mónica
author_facet	Piuri, Mariana Ruzal, Sandra Mónica
author_sort	Piuri, Mariana
title	Cell wall modifications during osmotic stress in Lactobacillus casei
title_short	Cell wall modifications during osmotic stress in Lactobacillus casei
title_full	Cell wall modifications during osmotic stress in Lactobacillus casei
title_fullStr	Cell wall modifications during osmotic stress in Lactobacillus casei
title_full_unstemmed	Cell wall modifications during osmotic stress in Lactobacillus casei
title_sort	cell wall modifications during osmotic stress in lactobacillus casei
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v98_n1_p84_Piuri http://hdl.handle.net/20.500.12110/paper_13645072_v98_n1_p84_Piuri
work_keys_str_mv	AT piurimariana cellwallmodificationsduringosmoticstressinlactobacilluscasei AT ruzalsandramonica cellwallmodificationsduringosmoticstressinlactobacilluscasei
_version_	1768542706009636864

Cell wall modifications during osmotic stress in Lactobacillus casei

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