Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents
Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2)...
Guardado en:
| Publicado: |
2016
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v121_n6_p1766_Magnoli http://hdl.handle.net/20.500.12110/paper_13645072_v121_n6_p1766_Magnoli |
| Aporte de: |
| id |
paper:paper_13645072_v121_n6_p1766_Magnoli |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_13645072_v121_n6_p1766_Magnoli2023-06-08T16:11:45Z Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents adsorption aflatoxin B1 broilers’ ecosystem native yeast Aves Candida tropicalis Clavispora lusitaniae Pichia aflatoxin B1 adsorption animal animal food chicken feces intestine isolation and purification metabolism microbiology yeast Adsorption Aflatoxin B1 Animal Feed Animals Chickens Feces Intestines Yeasts Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml−1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This study makes a contribution to using native yeasts from broilers’ environment for controlling chronic aflatoxicosis in avian production. © 2016 The Society for Applied Microbiology 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v121_n6_p1766_Magnoli http://hdl.handle.net/20.500.12110/paper_13645072_v121_n6_p1766_Magnoli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adsorption aflatoxin B1 broilers’ ecosystem native yeast Aves Candida tropicalis Clavispora lusitaniae Pichia aflatoxin B1 adsorption animal animal food chicken feces intestine isolation and purification metabolism microbiology yeast Adsorption Aflatoxin B1 Animal Feed Animals Chickens Feces Intestines Yeasts |
| spellingShingle |
adsorption aflatoxin B1 broilers’ ecosystem native yeast Aves Candida tropicalis Clavispora lusitaniae Pichia aflatoxin B1 adsorption animal animal food chicken feces intestine isolation and purification metabolism microbiology yeast Adsorption Aflatoxin B1 Animal Feed Animals Chickens Feces Intestines Yeasts Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents |
| topic_facet |
adsorption aflatoxin B1 broilers’ ecosystem native yeast Aves Candida tropicalis Clavispora lusitaniae Pichia aflatoxin B1 adsorption animal animal food chicken feces intestine isolation and purification metabolism microbiology yeast Adsorption Aflatoxin B1 Animal Feed Animals Chickens Feces Intestines Yeasts |
| description |
Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml−1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This study makes a contribution to using native yeasts from broilers’ environment for controlling chronic aflatoxicosis in avian production. © 2016 The Society for Applied Microbiology |
| title |
Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents |
| title_short |
Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents |
| title_full |
Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents |
| title_fullStr |
Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents |
| title_full_unstemmed |
Novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin B1 adsorbents |
| title_sort |
novel yeast isolated from broilers’ feedstuff, gut and faeces as aflatoxin b1 adsorbents |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v121_n6_p1766_Magnoli http://hdl.handle.net/20.500.12110/paper_13645072_v121_n6_p1766_Magnoli |
| _version_ |
1768544697693765632 |