Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1

Aim: To evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B1 (FB1) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology. Methods and Results: Using Plackett-Burman screening designs (PBSD) and cen...

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Publicado:	2013
Materias:	Biomass production Cane molasses Fumonisin B1 Saccharomyces cerevisiae Statistical optimization fumonisin B1 molasses probiotic agent bioassay biomass concentration (composition) fermentation growth rate optimization probiotics statistical analysis toxic organism yeast article batch fermentation binding assay biomass production bioreactor chemical structure culture medium fungal strain fungus culture fungus growth in vitro study incubation time nonhuman response surface method sugarcane Animal Feed Biomass Bioreactors Carbon Culture Media Fermentation Fumonisins Industrial Microbiology Models, Statistical Molasses Nitrogen Probiotics Saccharum Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v114_n5_p1338_Armando http://hdl.handle.net/20.500.12110/paper_13645072_v114_n5_p1338_Armando
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13645072_v114_n5_p1338_Armando
record_format	dspace
spelling	paper:paper_13645072_v114_n5_p1338_Armando2023-06-08T16:11:45Z Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1 Biomass production Cane molasses Fumonisin B1 Saccharomyces cerevisiae Statistical optimization fumonisin B1 molasses probiotic agent bioassay biomass concentration (composition) fermentation growth rate optimization probiotics statistical analysis toxic organism yeast article batch fermentation binding assay biomass production bioreactor chemical structure culture medium fungal strain fungus culture fungus growth in vitro study incubation time nonhuman response surface method Saccharomyces cerevisiae sugarcane Animal Feed Biomass Bioreactors Carbon Culture Media Fermentation Fumonisins Industrial Microbiology Models, Statistical Molasses Nitrogen Probiotics Saccharomyces cerevisiae Saccharum Animalia Saccharomyces cerevisiae Saccharum Aim: To evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B1 (FB1) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology. Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (g l-1) fermentable sugars provided by sugar cane molasses (CMs), yeast extract (YE) and (NH4)2HPO4 (DAP) was formulated. The S. cerevisiae RC016 strain showed the greatest binding at all assayed FB1 concentration. The CMs, YE, DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC016. Conclusion: A combination of CMs 17%; YE 4·61 g l-1 and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC016. Significance and Impact of the Study: The importance of this work lies in the search for live strains with both probiotic and fumonisin B1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed. © 2013 The Society for Applied Microbiology. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v114_n5_p1338_Armando http://hdl.handle.net/20.500.12110/paper_13645072_v114_n5_p1338_Armando
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biomass production Cane molasses Fumonisin B1 Saccharomyces cerevisiae Statistical optimization fumonisin B1 molasses probiotic agent bioassay biomass concentration (composition) fermentation growth rate optimization probiotics statistical analysis toxic organism yeast article batch fermentation binding assay biomass production bioreactor chemical structure culture medium fungal strain fungus culture fungus growth in vitro study incubation time nonhuman response surface method Saccharomyces cerevisiae sugarcane Animal Feed Biomass Bioreactors Carbon Culture Media Fermentation Fumonisins Industrial Microbiology Models, Statistical Molasses Nitrogen Probiotics Saccharomyces cerevisiae Saccharum Animalia Saccharomyces cerevisiae Saccharum
spellingShingle	Biomass production Cane molasses Fumonisin B1 Saccharomyces cerevisiae Statistical optimization fumonisin B1 molasses probiotic agent bioassay biomass concentration (composition) fermentation growth rate optimization probiotics statistical analysis toxic organism yeast article batch fermentation binding assay biomass production bioreactor chemical structure culture medium fungal strain fungus culture fungus growth in vitro study incubation time nonhuman response surface method Saccharomyces cerevisiae sugarcane Animal Feed Biomass Bioreactors Carbon Culture Media Fermentation Fumonisins Industrial Microbiology Models, Statistical Molasses Nitrogen Probiotics Saccharomyces cerevisiae Saccharum Animalia Saccharomyces cerevisiae Saccharum Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
topic_facet	Biomass production Cane molasses Fumonisin B1 Saccharomyces cerevisiae Statistical optimization fumonisin B1 molasses probiotic agent bioassay biomass concentration (composition) fermentation growth rate optimization probiotics statistical analysis toxic organism yeast article batch fermentation binding assay biomass production bioreactor chemical structure culture medium fungal strain fungus culture fungus growth in vitro study incubation time nonhuman response surface method Saccharomyces cerevisiae sugarcane Animal Feed Biomass Bioreactors Carbon Culture Media Fermentation Fumonisins Industrial Microbiology Models, Statistical Molasses Nitrogen Probiotics Saccharomyces cerevisiae Saccharum Animalia Saccharomyces cerevisiae Saccharum
description	Aim: To evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B1 (FB1) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology. Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (g l-1) fermentable sugars provided by sugar cane molasses (CMs), yeast extract (YE) and (NH4)2HPO4 (DAP) was formulated. The S. cerevisiae RC016 strain showed the greatest binding at all assayed FB1 concentration. The CMs, YE, DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC016. Conclusion: A combination of CMs 17%; YE 4·61 g l-1 and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC016. Significance and Impact of the Study: The importance of this work lies in the search for live strains with both probiotic and fumonisin B1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed. © 2013 The Society for Applied Microbiology.
title	Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
title_short	Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
title_full	Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
title_fullStr	Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
title_full_unstemmed	Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
title_sort	statistical optimization of culture conditions for biomass production of probiotic gut-borne saccharomyces cerevisiae strain able to reduce fumonisin b1
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v114_n5_p1338_Armando http://hdl.handle.net/20.500.12110/paper_13645072_v114_n5_p1338_Armando
_version_	1768544609937391616

Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1

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