Occurrence of GABA and GABA receptors in human spermatozoa
Gamma-aminobutyric acid (GABA) concentrations in seminal plasma and washed spermatozoa from normal donors were assessed by a sensitive radioreceptor assay, and were detectable in both fractions. Specific binding of [3H]-muscimol was shown to be dependent on protein concentration, temperature and inc...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v4_n8_p769_Ritta http://hdl.handle.net/20.500.12110/paper_13609947_v4_n8_p769_Ritta |
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paper:paper_13609947_v4_n8_p769_Ritta2025-07-30T18:47:24Z Occurrence of GABA and GABA receptors in human spermatozoa GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility Gamma-aminobutyric acid (GABA) concentrations in seminal plasma and washed spermatozoa from normal donors were assessed by a sensitive radioreceptor assay, and were detectable in both fractions. Specific binding of [3H]-muscimol was shown to be dependent on protein concentration, temperature and incubation time. [3H]-muscimol specific binding to human sperm membranes was significantly inhibited by the GABA type A receptor (GABA(A)) antagonist, bicuculline, and by the GABA(A) agonists, muscimol and isoguvacine, but not by the GABA type B receptor (GABA(B)) agonist baclofen. Scatchard analysis of [3H]-muscimol binding yielded a linear plot consistent with a single population of binding sites with a dissociation constant in the low nanomolar range. Incubation with GABA at a high micromolar concentration for 3 h under capacitating conditions resulted in an increase in the percentage of spermatozoa showing hyperactivated motility as assessed by computerized motility analyser. However, low micromolar concentrations of the GABA(A) agonist, muscimol, were sufficient to significantly increase sperm hyperactivity. These results suggest that the effect of GABA on human sperm motility might be mediated through a specific GABA(A) receptor. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v4_n8_p769_Ritta http://hdl.handle.net/20.500.12110/paper_13609947_v4_n8_p769_Ritta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility |
| spellingShingle |
GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility Occurrence of GABA and GABA receptors in human spermatozoa |
| topic_facet |
GABA Hyperactivated motility Specific binding Spermatozoa 4 aminobutyric acid 4 aminobutyric acid A receptor 4 aminobutyric acid B receptor 4 aminobutyric acid receptor bicuculline muscimol article controlled study dissociation constant human human cell male normal human priority journal protein expression receptor binding receptor density seminal plasma spermatozoon spermatozoon motility |
| description |
Gamma-aminobutyric acid (GABA) concentrations in seminal plasma and washed spermatozoa from normal donors were assessed by a sensitive radioreceptor assay, and were detectable in both fractions. Specific binding of [3H]-muscimol was shown to be dependent on protein concentration, temperature and incubation time. [3H]-muscimol specific binding to human sperm membranes was significantly inhibited by the GABA type A receptor (GABA(A)) antagonist, bicuculline, and by the GABA(A) agonists, muscimol and isoguvacine, but not by the GABA type B receptor (GABA(B)) agonist baclofen. Scatchard analysis of [3H]-muscimol binding yielded a linear plot consistent with a single population of binding sites with a dissociation constant in the low nanomolar range. Incubation with GABA at a high micromolar concentration for 3 h under capacitating conditions resulted in an increase in the percentage of spermatozoa showing hyperactivated motility as assessed by computerized motility analyser. However, low micromolar concentrations of the GABA(A) agonist, muscimol, were sufficient to significantly increase sperm hyperactivity. These results suggest that the effect of GABA on human sperm motility might be mediated through a specific GABA(A) receptor. |
| title |
Occurrence of GABA and GABA receptors in human spermatozoa |
| title_short |
Occurrence of GABA and GABA receptors in human spermatozoa |
| title_full |
Occurrence of GABA and GABA receptors in human spermatozoa |
| title_fullStr |
Occurrence of GABA and GABA receptors in human spermatozoa |
| title_full_unstemmed |
Occurrence of GABA and GABA receptors in human spermatozoa |
| title_sort |
occurrence of gaba and gaba receptors in human spermatozoa |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v4_n8_p769_Ritta http://hdl.handle.net/20.500.12110/paper_13609947_v4_n8_p769_Ritta |
| _version_ |
1840325960062205952 |