GnRH receptors and GnRH endocrine effects on luteoma cells

An ovary implanted into the spleen of an ovariectomized rat develops into a luteinized tumor, growing in response to gonadotrophins. Previously, it was shown that in vivo Buserelin, a gonadotrophin-releasing hormone (GnRH) analog, inhibited tumor growth. To determine if GnRH had a direct effect on t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Publicado:	1997
Materias:	Buserelin effects Estradiol GnRH receptors Luteoma Progesterone buserelin estradiol gonadorelin receptor progesterone animal experiment animal model animal tissue article controlled study female luteoma nonhuman priority journal rat subcutaneous drug administration tumor cell Animals Antineoplastic Agents, Hormonal Buserelin Estrus Female Gonadotropin-Releasing Hormone Iodine Radioisotopes Kinetics Organ Culture Techniques Ovarian Neoplasms Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p165_ChamsonReig http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p165_ChamsonReig
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1355008X_v6_n2_p165_ChamsonReig
record_format	dspace
spelling	paper:paper_1355008X_v6_n2_p165_ChamsonReig2023-06-08T16:11:07Z GnRH receptors and GnRH endocrine effects on luteoma cells Buserelin effects Estradiol GnRH receptors Luteoma Progesterone buserelin estradiol gonadorelin receptor progesterone animal experiment animal model animal tissue article controlled study female luteoma nonhuman priority journal rat subcutaneous drug administration tumor cell Animals Antineoplastic Agents, Hormonal Buserelin Estradiol Estrus Female Gonadotropin-Releasing Hormone Iodine Radioisotopes Kinetics Luteoma Organ Culture Techniques Ovarian Neoplasms Progesterone Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Tumor Cells, Cultured An ovary implanted into the spleen of an ovariectomized rat develops into a luteinized tumor, growing in response to gonadotrophins. Previously, it was shown that in vivo Buserelin, a gonadotrophin-releasing hormone (GnRH) analog, inhibited tumor growth. To determine if GnRH had a direct effect on tumor cells, the presence of GnRH receptors as well as the endocrine effects of buserelin were studied on tumoral tissue. GnRH receptors were present in luteoma in similar concentrations and dissociation constant (Kd) to control estrous ovaries. In vivo treatment with buserelin did not modify luteoma GnRH receptors, In organ incubations, luteoma secreted significantly higher estradiol and lower progesterone than estrous ovaries; addition of buserelin did not modify steroid secretion. The same difference in basal steroid secretion between luteoma cells and luteal cells superovulated prepubertal ovaries was observed in cell cultures. Although luteinizing-hormone (LH)-stimulated progesterone in both kinds of cells, buserelin significantly inhibited LH-stimulated progesterone only in luteoma cells. These results describe clear differences in basal steroid secretion between tumoral and normal tissue. Furthermore, they show that luteoma possess GnRH receptors similar to those in normal ovarian tissue, and that GnRH analogs have endocrine effects on these cells. Therefore, a direct effect of buserelin on luteoma cells can be postulated. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p165_ChamsonReig http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p165_ChamsonReig
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Buserelin effects Estradiol GnRH receptors Luteoma Progesterone buserelin estradiol gonadorelin receptor progesterone animal experiment animal model animal tissue article controlled study female luteoma nonhuman priority journal rat subcutaneous drug administration tumor cell Animals Antineoplastic Agents, Hormonal Buserelin Estradiol Estrus Female Gonadotropin-Releasing Hormone Iodine Radioisotopes Kinetics Luteoma Organ Culture Techniques Ovarian Neoplasms Progesterone Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Tumor Cells, Cultured
spellingShingle	Buserelin effects Estradiol GnRH receptors Luteoma Progesterone buserelin estradiol gonadorelin receptor progesterone animal experiment animal model animal tissue article controlled study female luteoma nonhuman priority journal rat subcutaneous drug administration tumor cell Animals Antineoplastic Agents, Hormonal Buserelin Estradiol Estrus Female Gonadotropin-Releasing Hormone Iodine Radioisotopes Kinetics Luteoma Organ Culture Techniques Ovarian Neoplasms Progesterone Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Tumor Cells, Cultured GnRH receptors and GnRH endocrine effects on luteoma cells
topic_facet	Buserelin effects Estradiol GnRH receptors Luteoma Progesterone buserelin estradiol gonadorelin receptor progesterone animal experiment animal model animal tissue article controlled study female luteoma nonhuman priority journal rat subcutaneous drug administration tumor cell Animals Antineoplastic Agents, Hormonal Buserelin Estradiol Estrus Female Gonadotropin-Releasing Hormone Iodine Radioisotopes Kinetics Luteoma Organ Culture Techniques Ovarian Neoplasms Progesterone Rats Rats, Sprague-Dawley Receptors, LHRH Superovulation Tumor Cells, Cultured
description	An ovary implanted into the spleen of an ovariectomized rat develops into a luteinized tumor, growing in response to gonadotrophins. Previously, it was shown that in vivo Buserelin, a gonadotrophin-releasing hormone (GnRH) analog, inhibited tumor growth. To determine if GnRH had a direct effect on tumor cells, the presence of GnRH receptors as well as the endocrine effects of buserelin were studied on tumoral tissue. GnRH receptors were present in luteoma in similar concentrations and dissociation constant (Kd) to control estrous ovaries. In vivo treatment with buserelin did not modify luteoma GnRH receptors, In organ incubations, luteoma secreted significantly higher estradiol and lower progesterone than estrous ovaries; addition of buserelin did not modify steroid secretion. The same difference in basal steroid secretion between luteoma cells and luteal cells superovulated prepubertal ovaries was observed in cell cultures. Although luteinizing-hormone (LH)-stimulated progesterone in both kinds of cells, buserelin significantly inhibited LH-stimulated progesterone only in luteoma cells. These results describe clear differences in basal steroid secretion between tumoral and normal tissue. Furthermore, they show that luteoma possess GnRH receptors similar to those in normal ovarian tissue, and that GnRH analogs have endocrine effects on these cells. Therefore, a direct effect of buserelin on luteoma cells can be postulated.
title	GnRH receptors and GnRH endocrine effects on luteoma cells
title_short	GnRH receptors and GnRH endocrine effects on luteoma cells
title_full	GnRH receptors and GnRH endocrine effects on luteoma cells
title_fullStr	GnRH receptors and GnRH endocrine effects on luteoma cells
title_full_unstemmed	GnRH receptors and GnRH endocrine effects on luteoma cells
title_sort	gnrh receptors and gnrh endocrine effects on luteoma cells
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p165_ChamsonReig http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p165_ChamsonReig
_version_	1768545941793538048

GnRH receptors and GnRH endocrine effects on luteoma cells

Ejemplares similares