N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation

Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAO...

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Autores principales:	Calvo, Juan Carlos, Guerra, Liliana Noemí
Publicado:	2017
Materias:	3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line animal cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v22_n6_p265_Soto http://hdl.handle.net/20.500.12110/paper_13510002_v22_n6_p265_Soto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13510002_v22_n6_p265_Soto
record_format	dspace
spelling	paper:paper_13510002_v22_n6_p265_Soto2023-06-08T16:10:58Z N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation Calvo, Juan Carlos Guerra, Liliana Noemí 3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210–218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation. © 2016 Informa UK Limited, trading as Taylor & Francis Group. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v22_n6_p265_Soto http://hdl.handle.net/20.500.12110/paper_13510002_v22_n6_p265_Soto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation
spellingShingle	3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation Calvo, Juan Carlos Guerra, Liliana Noemí N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
topic_facet	3T3-L1 adipocyte differentiation Antioxidant kinase phosphorylation MAPK mitocondria monoamine oxidase A obesity acetylcysteine amine oxidase (flavin containing) isoenzyme A citrate synthase fumarate hydratase mitochondrial protein mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 stress activated protein kinase 1 acetylcysteine amine oxidase (flavin containing) antioxidant adipocyte adipogenesis Article cell count cell differentiation citric acid cycle controlled study mitochondrion mitosis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 cell line adipocyte animal cell differentiation cytology drug effect metabolism mouse phosphorylation 3T3-L1 Cells Acetylcysteine Adipocytes Animals Antioxidants Cell Differentiation Mice Monoamine Oxidase Phosphorylation
description	Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210–218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
author	Calvo, Juan Carlos Guerra, Liliana Noemí
author_facet	Calvo, Juan Carlos Guerra, Liliana Noemí
author_sort	Calvo, Juan Carlos
title	N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
title_short	N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
title_full	N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
title_fullStr	N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
title_full_unstemmed	N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
title_sort	n-acetylcysteine inhibits kinase phosphorylation during 3t3-l1 adipocyte differentiation
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v22_n6_p265_Soto http://hdl.handle.net/20.500.12110/paper_13510002_v22_n6_p265_Soto
work_keys_str_mv	AT calvojuancarlos nacetylcysteineinhibitskinasephosphorylationduring3t3l1adipocytedifferentiation AT guerraliliananoemi nacetylcysteineinhibitskinasephosphorylationduring3t3l1adipocytedifferentiation
_version_	1768542095763570688

N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation

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