Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction
Objectives: This study was focused on the role of indole acetic acid (IAA) in the defense against oxidative stress damage caused by drought in soybean plants and to elucidate whether heme oxygenase-1 (HO-1) and nitric oxide (NO) are involved in this mechanism. IAA is an auxin that participates in ma...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v19_n6_p242_Lecube http://hdl.handle.net/20.500.12110/paper_13510002_v19_n6_p242_Lecube |
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paper:paper_13510002_v19_n6_p242_Lecube2023-06-08T16:10:58Z Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction Drought stress Glycine max. L Heme oxygenase Indole acetic acid Nitric oxide ascorbic acid catalase glutathione guaiacol peroxidase heme oxygenase 1 indoleacetic acid macrogol nitric oxide nitroprusside sodium protoporphyrin zinc superoxide dismutase thiobarbituric acid reactive substance zinc ion antioxidant heme oxygenase indoleacetic acid indoleacetic acid derivative macrogol derivative Article drought stress enzyme activity gene expression lipid peroxidation nonhuman oxidative stress plant parameters protein content soybean chemistry drought enzymology metabolism physiological stress soybean Glycine max Antioxidants Ascorbic Acid Droughts Glutathione Heme Oxygenase (Decyclizing) Indoleacetic Acids Lipid Peroxidation Nitric Oxide Oxidative Stress Polyethylene Glycols Soybeans Stress, Physiological Thiobarbituric Acid Reactive Substances Objectives: This study was focused on the role of indole acetic acid (IAA) in the defense against oxidative stress damage caused by drought in soybean plants and to elucidate whether heme oxygenase-1 (HO-1) and nitric oxide (NO) are involved in this mechanism. IAA is an auxin that participates in many plant processes including oxidative stress defense, but to the best of our knowledge no information is yet available about its possible action in drought stress. Methods: To this end, soybean plants were treated with 8% polyethylene glycol (PEG) or 100 μM IAA. To evaluate the behavior of IAA, plants were pretreated with this compound previous to PEG addition. Lipid peroxidation levels (thiobarbituric acid reactive substances (TBARS)), glutathione (GSH) and ascorbate (AS) contents, catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) activities were determined to evaluate oxidative damage. Results: Drought treatment (8% PEG) caused a significant increase in TBARS levels as well as a marked decrease in the non-enzymatic (GSH and AS) and enzymatic (CAT, SOD, and POD) antioxidant defense systems. Pre-treatment with IAA prevented the alterations of stress parameters caused by drought, while treatment with IAA alone did not produce changes in TBARS levels, or GSH and AS contents. Moreover, the activities of the classical enzymes involved in the enzymatic defense system (SOD, CAT, and POD) remained similar to control values. Furthermore, this hormone could enhance HO-1 activity (75% with respect to controls), and this increase was positively correlated with protein content as well as gene expression. The direct participation of HO-1 as an antioxidant enzyme was established by performing experiments in the presence of Zn-protoporphyrin IX, a well-known irreversible inhibitor of this enzyme. It was also demonstrated that HO-1 is modulated by NO, as shown by experiments performed in the presence of an NO donor (sodium nitroprusside), an NO scavenger (2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide), or an NO synthesis inhibitor (N-nitro-L-arginine methyl ester, NAME). Discussion: It is concluded that IAA is responsible, at least in part, for the protection against oxidative stress caused by drought in soybean plants through the modulation of NO levels which, in turn, enhances HO-1 synthesis and activity. © W. S. Maney & Son Ltd 2014. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v19_n6_p242_Lecube http://hdl.handle.net/20.500.12110/paper_13510002_v19_n6_p242_Lecube |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Drought stress Glycine max. L Heme oxygenase Indole acetic acid Nitric oxide ascorbic acid catalase glutathione guaiacol peroxidase heme oxygenase 1 indoleacetic acid macrogol nitric oxide nitroprusside sodium protoporphyrin zinc superoxide dismutase thiobarbituric acid reactive substance zinc ion antioxidant heme oxygenase indoleacetic acid indoleacetic acid derivative macrogol derivative Article drought stress enzyme activity gene expression lipid peroxidation nonhuman oxidative stress plant parameters protein content soybean chemistry drought enzymology metabolism physiological stress soybean Glycine max Antioxidants Ascorbic Acid Droughts Glutathione Heme Oxygenase (Decyclizing) Indoleacetic Acids Lipid Peroxidation Nitric Oxide Oxidative Stress Polyethylene Glycols Soybeans Stress, Physiological Thiobarbituric Acid Reactive Substances |
| spellingShingle |
Drought stress Glycine max. L Heme oxygenase Indole acetic acid Nitric oxide ascorbic acid catalase glutathione guaiacol peroxidase heme oxygenase 1 indoleacetic acid macrogol nitric oxide nitroprusside sodium protoporphyrin zinc superoxide dismutase thiobarbituric acid reactive substance zinc ion antioxidant heme oxygenase indoleacetic acid indoleacetic acid derivative macrogol derivative Article drought stress enzyme activity gene expression lipid peroxidation nonhuman oxidative stress plant parameters protein content soybean chemistry drought enzymology metabolism physiological stress soybean Glycine max Antioxidants Ascorbic Acid Droughts Glutathione Heme Oxygenase (Decyclizing) Indoleacetic Acids Lipid Peroxidation Nitric Oxide Oxidative Stress Polyethylene Glycols Soybeans Stress, Physiological Thiobarbituric Acid Reactive Substances Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction |
| topic_facet |
Drought stress Glycine max. L Heme oxygenase Indole acetic acid Nitric oxide ascorbic acid catalase glutathione guaiacol peroxidase heme oxygenase 1 indoleacetic acid macrogol nitric oxide nitroprusside sodium protoporphyrin zinc superoxide dismutase thiobarbituric acid reactive substance zinc ion antioxidant heme oxygenase indoleacetic acid indoleacetic acid derivative macrogol derivative Article drought stress enzyme activity gene expression lipid peroxidation nonhuman oxidative stress plant parameters protein content soybean chemistry drought enzymology metabolism physiological stress soybean Glycine max Antioxidants Ascorbic Acid Droughts Glutathione Heme Oxygenase (Decyclizing) Indoleacetic Acids Lipid Peroxidation Nitric Oxide Oxidative Stress Polyethylene Glycols Soybeans Stress, Physiological Thiobarbituric Acid Reactive Substances |
| description |
Objectives: This study was focused on the role of indole acetic acid (IAA) in the defense against oxidative stress damage caused by drought in soybean plants and to elucidate whether heme oxygenase-1 (HO-1) and nitric oxide (NO) are involved in this mechanism. IAA is an auxin that participates in many plant processes including oxidative stress defense, but to the best of our knowledge no information is yet available about its possible action in drought stress. Methods: To this end, soybean plants were treated with 8% polyethylene glycol (PEG) or 100 μM IAA. To evaluate the behavior of IAA, plants were pretreated with this compound previous to PEG addition. Lipid peroxidation levels (thiobarbituric acid reactive substances (TBARS)), glutathione (GSH) and ascorbate (AS) contents, catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) activities were determined to evaluate oxidative damage. Results: Drought treatment (8% PEG) caused a significant increase in TBARS levels as well as a marked decrease in the non-enzymatic (GSH and AS) and enzymatic (CAT, SOD, and POD) antioxidant defense systems. Pre-treatment with IAA prevented the alterations of stress parameters caused by drought, while treatment with IAA alone did not produce changes in TBARS levels, or GSH and AS contents. Moreover, the activities of the classical enzymes involved in the enzymatic defense system (SOD, CAT, and POD) remained similar to control values. Furthermore, this hormone could enhance HO-1 activity (75% with respect to controls), and this increase was positively correlated with protein content as well as gene expression. The direct participation of HO-1 as an antioxidant enzyme was established by performing experiments in the presence of Zn-protoporphyrin IX, a well-known irreversible inhibitor of this enzyme. It was also demonstrated that HO-1 is modulated by NO, as shown by experiments performed in the presence of an NO donor (sodium nitroprusside), an NO scavenger (2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide), or an NO synthesis inhibitor (N-nitro-L-arginine methyl ester, NAME). Discussion: It is concluded that IAA is responsible, at least in part, for the protection against oxidative stress caused by drought in soybean plants through the modulation of NO levels which, in turn, enhances HO-1 synthesis and activity. © W. S. Maney & Son Ltd 2014. |
| title |
Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction |
| title_short |
Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction |
| title_full |
Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction |
| title_fullStr |
Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction |
| title_full_unstemmed |
Indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: The role of heme oxygenase induction |
| title_sort |
indole acetic acid is responsible for protection against oxidative stress caused by drought in soybean plants: the role of heme oxygenase induction |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v19_n6_p242_Lecube http://hdl.handle.net/20.500.12110/paper_13510002_v19_n6_p242_Lecube |
| _version_ |
1768542048969818112 |