Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis

Most cells are naturally resistant to TNF-α-induced cell death and become sensitized when NF-κB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNIF-α-induced c...

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Autores principales:	Franco, Diana Lorena, Nojek, Ignacio Martín, Molinero, Luciana Lorena, Coso, Omar Adrian, Costas, Mónica Alejandra
Publicado:	2002
Materias:	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13509047_v9_n10_p1090_Franco http://hdl.handle.net/20.500.12110/paper_13509047_v9_n10_p1090_Franco
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13509047_v9_n10_p1090_Franco
record_format	dspace
spelling	paper:paper_13509047_v9_n10_p1090_Franco2023-06-08T16:10:57Z Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis Franco, Diana Lorena Nojek, Ignacio Martín Molinero, Luciana Lorena Coso, Omar Adrian Costas, Mónica Alejandra Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha Most cells are naturally resistant to TNF-α-induced cell death and become sensitized when NF-κB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNIF-α-induced cell death. We found that it sensitizes the naturally resistant HeLa cells to TNF-α-induced apoptosis, with the involvement of an increase in the activity of several kinases, the inhibition of Bcl-2 expression, and a late increase on NF-κB activation. Cell death occurs regardless of the enhanced NF-κB activity, whose inhibition produces an increase in apoptosis. The inhibition of p38 kinase, also involved in NF-κB activation, significantly increases the effect of osmotic stress on TNF-α-induced cell death. Fil:Franco, D.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nojek, I.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molinero, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Costas, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13509047_v9_n10_p1090_Franco http://hdl.handle.net/20.500.12110/paper_13509047_v9_n10_p1090_Franco
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha
spellingShingle	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha Franco, Diana Lorena Nojek, Ignacio Martín Molinero, Luciana Lorena Coso, Omar Adrian Costas, Mónica Alejandra Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
topic_facet	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha
description	Most cells are naturally resistant to TNF-α-induced cell death and become sensitized when NF-κB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNIF-α-induced cell death. We found that it sensitizes the naturally resistant HeLa cells to TNF-α-induced apoptosis, with the involvement of an increase in the activity of several kinases, the inhibition of Bcl-2 expression, and a late increase on NF-κB activation. Cell death occurs regardless of the enhanced NF-κB activity, whose inhibition produces an increase in apoptosis. The inhibition of p38 kinase, also involved in NF-κB activation, significantly increases the effect of osmotic stress on TNF-α-induced cell death.
author	Franco, Diana Lorena Nojek, Ignacio Martín Molinero, Luciana Lorena Coso, Omar Adrian Costas, Mónica Alejandra
author_facet	Franco, Diana Lorena Nojek, Ignacio Martín Molinero, Luciana Lorena Coso, Omar Adrian Costas, Mónica Alejandra
author_sort	Franco, Diana Lorena
title	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_short	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_full	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_fullStr	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_full_unstemmed	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_sort	osmotic stress sensitizes naturally resistant cells to tnf-α-induced apoptosis
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13509047_v9_n10_p1090_Franco http://hdl.handle.net/20.500.12110/paper_13509047_v9_n10_p1090_Franco
work_keys_str_mv	AT francodianalorena osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT nojekignaciomartin osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT molinerolucianalorena osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT cosoomaradrian osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT costasmonicaalejandra osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis
_version_	1768544697148506112

Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis

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