Isolation, culture and characterization of a new strain of Euglena gracilis

A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for...

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Autores principales: Ruíz, Laura Beatriz, Rocchetta, Iara, Conforti, Visitación Teresa Dora
Publicado: 2004
Materias:
Bleached
Culture
Euglena gracilis
Lipid
Protein
Strain
Sugar
flagellate
river pollution
Euglena
Mastigophora (flagellates)
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13220829_v52_n2_p168_Ruiz
http://hdl.handle.net/20.500.12110/paper_13220829_v52_n2_p168_Ruiz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_13220829_v52_n2_p168_Ruiz
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spelling paper:paper_13220829_v52_n2_p168_Ruiz2023-06-08T16:10:23Z Isolation, culture and characterization of a new strain of Euglena gracilis Ruíz, Laura Beatriz Rocchetta, Iara Conforti, Visitación Teresa Dora Bleached Culture Euglena gracilis Lipid Protein Strain Sugar flagellate river pollution Euglena Euglena gracilis Euglena gracilis Mastigophora (flagellates) A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for MAT were compared with data obtained for a UTEX Culture Collection strain. Likewise, cells from both strains were bleached using streptomycin, and grown in the same media used for green samples. Both MAT and UTEX showed clear differences in their biochemical composition and growth rate depending on the media used. They also exhibited different growth patterns. E. gracilis medium proved to be the best culture environment for both strains either in autotrophic or heterotrophic conditions. Results show that basal contents of lipids, sugars, proteins and chlorophyll a vary depending on the strain, and thus values obtained for one strain do not apply to another. Moreover, strain origin may have an influence on the mechanisms of adaptation or defense developed by each sample. Fil:Ruiz, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Conforti, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13220829_v52_n2_p168_Ruiz http://hdl.handle.net/20.500.12110/paper_13220829_v52_n2_p168_Ruiz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bleached
Culture
Euglena gracilis
Lipid
Protein
Strain
Sugar
flagellate
river pollution
Euglena
Euglena gracilis
Euglena gracilis
Mastigophora (flagellates)
spellingShingle Bleached
Culture
Euglena gracilis
Lipid
Protein
Strain
Sugar
flagellate
river pollution
Euglena
Euglena gracilis
Euglena gracilis
Mastigophora (flagellates)
Ruíz, Laura Beatriz
Rocchetta, Iara
Conforti, Visitación Teresa Dora
Isolation, culture and characterization of a new strain of Euglena gracilis
topic_facet Bleached
Culture
Euglena gracilis
Lipid
Protein
Strain
Sugar
flagellate
river pollution
Euglena
Euglena gracilis
Euglena gracilis
Mastigophora (flagellates)
description A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for MAT were compared with data obtained for a UTEX Culture Collection strain. Likewise, cells from both strains were bleached using streptomycin, and grown in the same media used for green samples. Both MAT and UTEX showed clear differences in their biochemical composition and growth rate depending on the media used. They also exhibited different growth patterns. E. gracilis medium proved to be the best culture environment for both strains either in autotrophic or heterotrophic conditions. Results show that basal contents of lipids, sugars, proteins and chlorophyll a vary depending on the strain, and thus values obtained for one strain do not apply to another. Moreover, strain origin may have an influence on the mechanisms of adaptation or defense developed by each sample.
author Ruíz, Laura Beatriz
Rocchetta, Iara
Conforti, Visitación Teresa Dora
author_facet Ruíz, Laura Beatriz
Rocchetta, Iara
Conforti, Visitación Teresa Dora
author_sort Ruíz, Laura Beatriz
title Isolation, culture and characterization of a new strain of Euglena gracilis
title_short Isolation, culture and characterization of a new strain of Euglena gracilis
title_full Isolation, culture and characterization of a new strain of Euglena gracilis
title_fullStr Isolation, culture and characterization of a new strain of Euglena gracilis
title_full_unstemmed Isolation, culture and characterization of a new strain of Euglena gracilis
title_sort isolation, culture and characterization of a new strain of euglena gracilis
publishDate 2004
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13220829_v52_n2_p168_Ruiz
http://hdl.handle.net/20.500.12110/paper_13220829_v52_n2_p168_Ruiz
work_keys_str_mv AT ruizlaurabeatriz isolationcultureandcharacterizationofanewstrainofeuglenagracilis
AT rocchettaiara isolationcultureandcharacterizationofanewstrainofeuglenagracilis
AT confortivisitacionteresadora isolationcultureandcharacterizationofanewstrainofeuglenagracilis
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