Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?

Lactobacillus plantarum ST16PA was isolated from papaya and previously have been shown that produces a 6.5 kDa bacteriocin, active against different species from genera Enterobacter, Enterococcus, Lactobacillus, Pseudomonas, Streptococcus and Staphylococcus and different serotypes of Listeria spp. I...

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Guardado en:
Detalles Bibliográficos
Publicado: 2014
Materias:
Bacteriocin
Cytotoxicity
Lactobacillus plantarum
Virulence factors
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13100351_v20_n_p55_Todorov
http://hdl.handle.net/20.500.12110/paper_13100351_v20_n_p55_Todorov
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_13100351_v20_n_p55_Todorov
record_format dspace
spelling paper:paper_13100351_v20_n_p55_Todorov2023-06-08T16:10:18Z Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture? Bacteriocin Cytotoxicity Lactobacillus plantarum Virulence factors Lactobacillus plantarum ST16PA was isolated from papaya and previously have been shown that produces a 6.5 kDa bacteriocin, active against different species from genera Enterobacter, Enterococcus, Lactobacillus, Pseudomonas, Streptococcus and Staphylococcus and different serotypes of Listeria spp. In addition Lb. plantarum ST16Pa presented a good potential to be consider as a probiotic candidate based on the genetic and physiological tests. PCR analysis of DNA from Lb. plantarum ST16Pa was generated positive results for presence of nisin and enterocin P genes and no evidences for presence of pediocin PA-1, plantaricin S, plantaricin W, plantaricin NC8, enterocin A, enterocin B or enterocin L50B have been obtained. Based on the molecular size (6.5 kDa as determined by Tricin-SDS-PAGE) of the expressed bacteriocin, most probably Lb. plantarum ST16Pa express bacteriocin different from nisin or enterocin P. Semi-purified bacteriocin was presenting a very high activity against Listeria monocytogenes (102 400 AU ml–1), Peudomonas aeroginosa (25 600 AU ml–1) and Enterococcus faecalis (102 400 AU ml–1). However, when semi-purified bacteriocin have been tested for cytotoxicity, CC50 > 1600 μg ml–1 have been recorded. In addition, Lb. plantarum ST16Pa generated positive PCR results on the DNA level for gelE (gelatinase), hyl (hyaluronidase), asa1 (aggregation substance), ace (adhesion of collagen) and tdc (tyrosine decarboxylase), a high virulence profile when been examined for presence of virulence factors. © 2014, National Centre for Agrarian Sciences. All rights reserved. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13100351_v20_n_p55_Todorov http://hdl.handle.net/20.500.12110/paper_13100351_v20_n_p55_Todorov
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bacteriocin
Cytotoxicity
Lactobacillus plantarum
Virulence factors
spellingShingle Bacteriocin
Cytotoxicity
Lactobacillus plantarum
Virulence factors
Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?
topic_facet Bacteriocin
Cytotoxicity
Lactobacillus plantarum
Virulence factors
description Lactobacillus plantarum ST16PA was isolated from papaya and previously have been shown that produces a 6.5 kDa bacteriocin, active against different species from genera Enterobacter, Enterococcus, Lactobacillus, Pseudomonas, Streptococcus and Staphylococcus and different serotypes of Listeria spp. In addition Lb. plantarum ST16Pa presented a good potential to be consider as a probiotic candidate based on the genetic and physiological tests. PCR analysis of DNA from Lb. plantarum ST16Pa was generated positive results for presence of nisin and enterocin P genes and no evidences for presence of pediocin PA-1, plantaricin S, plantaricin W, plantaricin NC8, enterocin A, enterocin B or enterocin L50B have been obtained. Based on the molecular size (6.5 kDa as determined by Tricin-SDS-PAGE) of the expressed bacteriocin, most probably Lb. plantarum ST16Pa express bacteriocin different from nisin or enterocin P. Semi-purified bacteriocin was presenting a very high activity against Listeria monocytogenes (102 400 AU ml–1), Peudomonas aeroginosa (25 600 AU ml–1) and Enterococcus faecalis (102 400 AU ml–1). However, when semi-purified bacteriocin have been tested for cytotoxicity, CC50 > 1600 μg ml–1 have been recorded. In addition, Lb. plantarum ST16Pa generated positive PCR results on the DNA level for gelE (gelatinase), hyl (hyaluronidase), asa1 (aggregation substance), ace (adhesion of collagen) and tdc (tyrosine decarboxylase), a high virulence profile when been examined for presence of virulence factors. © 2014, National Centre for Agrarian Sciences. All rights reserved.
title Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?
title_short Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?
title_full Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?
title_fullStr Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?
title_full_unstemmed Lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?
title_sort lactobacillus plantarum st16pa–are we ready to use it as bio-protective culture?
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13100351_v20_n_p55_Todorov
http://hdl.handle.net/20.500.12110/paper_13100351_v20_n_p55_Todorov
_version_ 1768544652463439872

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