Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides

A primary mode-of-action of all pyrethroid insecticides (PYRs) is the disruption of the voltage-gated sodium channel electrophysiology in neurons of target pests and nontarget species. The neurological actions of PYRs on non-neuronal cells of the nervous system remain poorly investigated. In the pre...

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Autores principales:	Romero, Delfina M., Wolansky, Marcelo Javier, Kotler, Mónica Lidia
Publicado:	2017
Materias:	C6 cells Cell death Cellular damage Pyrethroids 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide bifenthrin cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug insecticide animal cell Article astrocytoma cell cell nucleus cell viability controlled study drug cytotoxicity glia cell lysosome mitochondrion MTT assay neurotoxicity nonhuman rat animal astrocytoma cell fractionation dose response fluorescence microscopy metabolism pathology tumor cell line Animals Astrocytoma Cell Line, Tumor Dose-Response Relationship, Drug Insecticides Microscopy, Fluorescence Pyrethrins Rats Subcellular Fractions
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10966080_v155_n1_p196_Romero http://hdl.handle.net/20.500.12110/paper_10966080_v155_n1_p196_Romero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10966080_v155_n1_p196_Romero
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spelling	paper:paper_10966080_v155_n1_p196_Romero2023-06-08T16:07:04Z Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides Romero, Delfina M. Wolansky, Marcelo Javier Kotler, Mónica Lidia C6 cells Cell death Cellular damage Pyrethroids 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide bifenthrin cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug insecticide pyrethroid animal cell Article astrocytoma cell cell nucleus cell viability controlled study drug cytotoxicity glia cell lysosome mitochondrion MTT assay neurotoxicity nonhuman rat animal astrocytoma cell fractionation dose response fluorescence microscopy metabolism pathology tumor cell line Animals Astrocytoma Cell Line, Tumor Dose-Response Relationship, Drug Insecticides Microscopy, Fluorescence Pyrethrins Rats Subcellular Fractions A primary mode-of-action of all pyrethroid insecticides (PYRs) is the disruption of the voltage-gated sodium channel electrophysiology in neurons of target pests and nontarget species. The neurological actions of PYRs on non-neuronal cells of the nervous system remain poorly investigated. In the present work, we used C6 astrocytoma cells to study PYR actions (0.1-50 μM) under the hypothesis that glial cells may be targeted by and vulnerable to PYRs. To this end, we characterized the effects of bifenthrin (BF), tefluthrin (TF), α-cypermethrin (α-CYP), and deltamethrin (DM) on the integrity of nuclear, mitochondrial, and lysosomal compartments. In general, 24- to 48-h exposures produced concentration-related impairment of cell viability. In single-compound, 24-h exposure experiments, effective concentration (EC)15s 3-(4,5-dimethyl-thiazol-2- yl)-2,5-diphenyl-tetrazolium bromide (MTT assay) were computed as follows (in μM): BF, 16.1; TF, 37.3; α-CYP, 7.8; DM, 5.0. We found concentration-related damage in several C6-cell subcellular compartments (mitochondria, nuclei, and lysosomes) at≥ 10-1 lM levels. Last, we examined a mixture of all PYRs (ie, Σ individual EC15) using MTT assays and subcellular analyses. Our findings indicate that C6 cells are responsive to nM levels of PYRs, suggesting that astroglial susceptibility may contribute to the low-dose neurological effects caused by these insecticides. This research further suggests that C6 cells may provide relevant information as a screening platform for pesticide mixtures targeting nervous system cells by expected and unexpected toxicogenic pathways potentially contributing to clinical neurotoxicity. © The Author 2016. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. Fil:Romero, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wolansky, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kotler, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10966080_v155_n1_p196_Romero http://hdl.handle.net/20.500.12110/paper_10966080_v155_n1_p196_Romero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	C6 cells Cell death Cellular damage Pyrethroids 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide bifenthrin cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug insecticide pyrethroid animal cell Article astrocytoma cell cell nucleus cell viability controlled study drug cytotoxicity glia cell lysosome mitochondrion MTT assay neurotoxicity nonhuman rat animal astrocytoma cell fractionation dose response fluorescence microscopy metabolism pathology tumor cell line Animals Astrocytoma Cell Line, Tumor Dose-Response Relationship, Drug Insecticides Microscopy, Fluorescence Pyrethrins Rats Subcellular Fractions
spellingShingle	C6 cells Cell death Cellular damage Pyrethroids 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide bifenthrin cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug insecticide pyrethroid animal cell Article astrocytoma cell cell nucleus cell viability controlled study drug cytotoxicity glia cell lysosome mitochondrion MTT assay neurotoxicity nonhuman rat animal astrocytoma cell fractionation dose response fluorescence microscopy metabolism pathology tumor cell line Animals Astrocytoma Cell Line, Tumor Dose-Response Relationship, Drug Insecticides Microscopy, Fluorescence Pyrethrins Rats Subcellular Fractions Romero, Delfina M. Wolansky, Marcelo Javier Kotler, Mónica Lidia Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides
topic_facet	C6 cells Cell death Cellular damage Pyrethroids 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide bifenthrin cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug insecticide pyrethroid animal cell Article astrocytoma cell cell nucleus cell viability controlled study drug cytotoxicity glia cell lysosome mitochondrion MTT assay neurotoxicity nonhuman rat animal astrocytoma cell fractionation dose response fluorescence microscopy metabolism pathology tumor cell line Animals Astrocytoma Cell Line, Tumor Dose-Response Relationship, Drug Insecticides Microscopy, Fluorescence Pyrethrins Rats Subcellular Fractions
description	A primary mode-of-action of all pyrethroid insecticides (PYRs) is the disruption of the voltage-gated sodium channel electrophysiology in neurons of target pests and nontarget species. The neurological actions of PYRs on non-neuronal cells of the nervous system remain poorly investigated. In the present work, we used C6 astrocytoma cells to study PYR actions (0.1-50 μM) under the hypothesis that glial cells may be targeted by and vulnerable to PYRs. To this end, we characterized the effects of bifenthrin (BF), tefluthrin (TF), α-cypermethrin (α-CYP), and deltamethrin (DM) on the integrity of nuclear, mitochondrial, and lysosomal compartments. In general, 24- to 48-h exposures produced concentration-related impairment of cell viability. In single-compound, 24-h exposure experiments, effective concentration (EC)15s 3-(4,5-dimethyl-thiazol-2- yl)-2,5-diphenyl-tetrazolium bromide (MTT assay) were computed as follows (in μM): BF, 16.1; TF, 37.3; α-CYP, 7.8; DM, 5.0. We found concentration-related damage in several C6-cell subcellular compartments (mitochondria, nuclei, and lysosomes) at≥ 10-1 lM levels. Last, we examined a mixture of all PYRs (ie, Σ individual EC15) using MTT assays and subcellular analyses. Our findings indicate that C6 cells are responsive to nM levels of PYRs, suggesting that astroglial susceptibility may contribute to the low-dose neurological effects caused by these insecticides. This research further suggests that C6 cells may provide relevant information as a screening platform for pesticide mixtures targeting nervous system cells by expected and unexpected toxicogenic pathways potentially contributing to clinical neurotoxicity. © The Author 2016. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
author	Romero, Delfina M. Wolansky, Marcelo Javier Kotler, Mónica Lidia
author_facet	Romero, Delfina M. Wolansky, Marcelo Javier Kotler, Mónica Lidia
author_sort	Romero, Delfina M.
title	Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides
title_short	Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides
title_full	Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides
title_fullStr	Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides
title_full_unstemmed	Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides
title_sort	vulnerability of c6 astrocytoma cells after single-compound and joint exposure to type i and type ii pyrethroid insecticides
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10966080_v155_n1_p196_Romero http://hdl.handle.net/20.500.12110/paper_10966080_v155_n1_p196_Romero
work_keys_str_mv	AT romerodelfinam vulnerabilityofc6astrocytomacellsaftersinglecompoundandjointexposuretotypeiandtypeiipyrethroidinsecticides AT wolanskymarcelojavier vulnerabilityofc6astrocytomacellsaftersinglecompoundandjointexposuretotypeiandtypeiipyrethroidinsecticides AT kotlermonicalidia vulnerabilityofc6astrocytomacellsaftersinglecompoundandjointexposuretotypeiandtypeiipyrethroidinsecticides
_version_	1768541573566431232

Vulnerability of C6 astrocytoma cells after single-compound and joint exposure to type I and type II pyrethroid insecticides

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