Improving image contrast in fluorescence microscopy with nanostructured substrates

Metallic and dielectric nanostructures can show sharp contrasted resonances, sensitive to the environment, and high field enhancement in sub-wavelength volumes. For this reason, these structures are commonly used as molecular sensors. Only few works have focused on their application in optical micro...

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Detalles Bibliográficos
Publicado: 2015
Materias:
Fluorescence
Fluorescence microscopy
Nanostructures
Refractive index
Titanium dioxide
Axial resolutions
Background noise
Fluorescence excitation
Metallodielectric
Molecular sensors
Nanostructured substrates
Nanostructured TiO
Total internal reflections
Substrates
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10944087_v23_n23_p29772_Brunstein
http://hdl.handle.net/20.500.12110/paper_10944087_v23_n23_p29772_Brunstein
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_10944087_v23_n23_p29772_Brunstein
record_format dspace
spelling paper:paper_10944087_v23_n23_p29772_Brunstein2023-06-08T16:06:55Z Improving image contrast in fluorescence microscopy with nanostructured substrates Fluorescence Fluorescence microscopy Nanostructures Refractive index Titanium dioxide Axial resolutions Background noise Fluorescence excitation Metallodielectric Molecular sensors Nanostructured substrates Nanostructured TiO Total internal reflections Substrates Metallic and dielectric nanostructures can show sharp contrasted resonances, sensitive to the environment, and high field enhancement in sub-wavelength volumes. For this reason, these structures are commonly used as molecular sensors. Only few works have focused on their application in optical microscopy, in particular in superresolution. In this work we have designed, fabricated and optically tested a nanostructured TiO2 substrate, fabricated by direct embossing of TiO2 derived film, as a substrate for fluorescence microscopy. Moreover, using numerical simulations, we have compared the signal to background noise with respect to other metallo-dielectric structures. We show that the TiO2 structure is a good candidate for reducing the thickness of the fluorescence excitation down to ∼100 nm. Therefore, this substrate can be used to obtain Total Internal Reflection (TIRF) axial resolution without a TIRF-Microscopy system. ©2015 Optical Society of America. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10944087_v23_n23_p29772_Brunstein http://hdl.handle.net/20.500.12110/paper_10944087_v23_n23_p29772_Brunstein
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Fluorescence
Fluorescence microscopy
Nanostructures
Refractive index
Titanium dioxide
Axial resolutions
Background noise
Fluorescence excitation
Metallodielectric
Molecular sensors
Nanostructured substrates
Nanostructured TiO
Total internal reflections
Substrates
spellingShingle Fluorescence
Fluorescence microscopy
Nanostructures
Refractive index
Titanium dioxide
Axial resolutions
Background noise
Fluorescence excitation
Metallodielectric
Molecular sensors
Nanostructured substrates
Nanostructured TiO
Total internal reflections
Substrates
Improving image contrast in fluorescence microscopy with nanostructured substrates
topic_facet Fluorescence
Fluorescence microscopy
Nanostructures
Refractive index
Titanium dioxide
Axial resolutions
Background noise
Fluorescence excitation
Metallodielectric
Molecular sensors
Nanostructured substrates
Nanostructured TiO
Total internal reflections
Substrates
description Metallic and dielectric nanostructures can show sharp contrasted resonances, sensitive to the environment, and high field enhancement in sub-wavelength volumes. For this reason, these structures are commonly used as molecular sensors. Only few works have focused on their application in optical microscopy, in particular in superresolution. In this work we have designed, fabricated and optically tested a nanostructured TiO2 substrate, fabricated by direct embossing of TiO2 derived film, as a substrate for fluorescence microscopy. Moreover, using numerical simulations, we have compared the signal to background noise with respect to other metallo-dielectric structures. We show that the TiO2 structure is a good candidate for reducing the thickness of the fluorescence excitation down to ∼100 nm. Therefore, this substrate can be used to obtain Total Internal Reflection (TIRF) axial resolution without a TIRF-Microscopy system. ©2015 Optical Society of America.
title Improving image contrast in fluorescence microscopy with nanostructured substrates
title_short Improving image contrast in fluorescence microscopy with nanostructured substrates
title_full Improving image contrast in fluorescence microscopy with nanostructured substrates
title_fullStr Improving image contrast in fluorescence microscopy with nanostructured substrates
title_full_unstemmed Improving image contrast in fluorescence microscopy with nanostructured substrates
title_sort improving image contrast in fluorescence microscopy with nanostructured substrates
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10944087_v23_n23_p29772_Brunstein
http://hdl.handle.net/20.500.12110/paper_10944087_v23_n23_p29772_Brunstein
_version_ 1768545155937206272

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