β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
The successful use of six β-carbolines (i.e. nor-harmane (9H-pyrido[3,4-b]indole), harmane (1-methyl-9H-pyrido[3,4-b]indole), harmine (7-methoxy-1-methyl-9H-pyrido[3,4-b]indole), harmol (1-methyl-9H-pyrido[3,4-b]indol-7-ol), harmaline (3,4-dihydro-7-methoxy-1-methyl-9H-pyrido[3,4-b]indole) and harma...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v32_n3_p287_Nonami http://hdl.handle.net/20.500.12110/paper_10765174_v32_n3_p287_Nonami |
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| Sumario: | The successful use of six β-carbolines (i.e. nor-harmane (9H-pyrido[3,4-b]indole), harmane (1-methyl-9H-pyrido[3,4-b]indole), harmine (7-methoxy-1-methyl-9H-pyrido[3,4-b]indole), harmol (1-methyl-9H-pyrido[3,4-b]indol-7-ol), harmaline (3,4-dihydro-7-methoxy-1-methyl-9H-pyrido[3,4-b]indole) and harmalol (3,4-dihydro-1-methyl-9H-pyrido[3,4-b]indol-7-ol)) is reported as matrices in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) (λ(exc) = 337 nm) for proteins (proteins checked: gramicidin S, bovine insulin, aprotinin, horse heart cytochrome c, ribonuclease A, lysozyme, myoglobin, trypsin, protease and bovine serum albumin) and sulfated oligosaccharides (λ-carrageenans of M(r) 549, 712, 1570 and 1733), using stainless-steel probes and membranes (poly(vinylidene difluoride) (PVDF) polymers) to prepare the samples. The possible use of some phenylcarbonyl compounds (hydroxyphenyl ketones, amino- and hydroxybenzoic acids) and a few carbazole derivatives as matrices is also discussed briefly. In addition, the usefulness of the new matrices in MALDI/TOF-MS (λ(exc) = 337 nm) of proteins and sulfated oligosaccharides is compared with those of classical MALDI matrices such as α-cyano-4-hydroxycinnamic acid, gentisic acid, sinapinic acid, 6-aza-2-thiothymine and 3-indole-trans-β-acrylic acid. Laser desorption/TOF-MS (λ(exc) = 337 nm) of the new matrices is also described. |
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