β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices

The successful use of six β-carbolines (i.e. nor-harmane (9H-pyrido[3,4-b]indole), harmane (1-methyl-9H-pyrido[3,4-b]indole), harmine (7-methoxy-1-methyl-9H-pyrido[3,4-b]indole), harmol (1-methyl-9H-pyrido[3,4-b]indol-7-ol), harmaline (3,4-dihydro-7-methoxy-1-methyl-9H-pyrido[3,4-b]indole) and harma...

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Autor principal:	Erra Balsells, Rosa
Publicado:	1997
Materias:	β-Carbolines 9H-Pyrido[3,4-b]indoles Benzoindoles Carbazoles Phenylcarbonyl compounds Pyridoindoles UV MALDI matric UV MALDI/TOF-MS matrix acetophenone derivative alkaloid alpha cyano 4 hydroxycinnamic acid benzoic acid derivative benzophenone derivative beta carboline derivative carbazole derivative gentisic acid article fluorescence mass spectrometry materials membrane priority journal protein analysis technique ultraviolet spectrophotometry
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v32_n3_p287_Nonami http://hdl.handle.net/20.500.12110/paper_10765174_v32_n3_p287_Nonami
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10765174_v32_n3_p287_Nonami
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spelling	paper:paper_10765174_v32_n3_p287_Nonami2023-06-08T16:05:21Z β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices Erra Balsells, Rosa β-Carbolines 9H-Pyrido[3,4-b]indoles Benzoindoles Carbazoles Phenylcarbonyl compounds Pyridoindoles UV MALDI matric UV MALDI/TOF-MS matrix acetophenone derivative alkaloid alpha cyano 4 hydroxycinnamic acid benzoic acid derivative benzophenone derivative beta carboline derivative carbazole derivative gentisic acid article fluorescence mass spectrometry materials membrane priority journal protein analysis technique ultraviolet spectrophotometry The successful use of six β-carbolines (i.e. nor-harmane (9H-pyrido[3,4-b]indole), harmane (1-methyl-9H-pyrido[3,4-b]indole), harmine (7-methoxy-1-methyl-9H-pyrido[3,4-b]indole), harmol (1-methyl-9H-pyrido[3,4-b]indol-7-ol), harmaline (3,4-dihydro-7-methoxy-1-methyl-9H-pyrido[3,4-b]indole) and harmalol (3,4-dihydro-1-methyl-9H-pyrido[3,4-b]indol-7-ol)) is reported as matrices in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) (λ(exc) = 337 nm) for proteins (proteins checked: gramicidin S, bovine insulin, aprotinin, horse heart cytochrome c, ribonuclease A, lysozyme, myoglobin, trypsin, protease and bovine serum albumin) and sulfated oligosaccharides (λ-carrageenans of M(r) 549, 712, 1570 and 1733), using stainless-steel probes and membranes (poly(vinylidene difluoride) (PVDF) polymers) to prepare the samples. The possible use of some phenylcarbonyl compounds (hydroxyphenyl ketones, amino- and hydroxybenzoic acids) and a few carbazole derivatives as matrices is also discussed briefly. In addition, the usefulness of the new matrices in MALDI/TOF-MS (λ(exc) = 337 nm) of proteins and sulfated oligosaccharides is compared with those of classical MALDI matrices such as α-cyano-4-hydroxycinnamic acid, gentisic acid, sinapinic acid, 6-aza-2-thiothymine and 3-indole-trans-β-acrylic acid. Laser desorption/TOF-MS (λ(exc) = 337 nm) of the new matrices is also described. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v32_n3_p287_Nonami http://hdl.handle.net/20.500.12110/paper_10765174_v32_n3_p287_Nonami
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-Carbolines 9H-Pyrido[3,4-b]indoles Benzoindoles Carbazoles Phenylcarbonyl compounds Pyridoindoles UV MALDI matric UV MALDI/TOF-MS matrix acetophenone derivative alkaloid alpha cyano 4 hydroxycinnamic acid benzoic acid derivative benzophenone derivative beta carboline derivative carbazole derivative gentisic acid article fluorescence mass spectrometry materials membrane priority journal protein analysis technique ultraviolet spectrophotometry
spellingShingle	β-Carbolines 9H-Pyrido[3,4-b]indoles Benzoindoles Carbazoles Phenylcarbonyl compounds Pyridoindoles UV MALDI matric UV MALDI/TOF-MS matrix acetophenone derivative alkaloid alpha cyano 4 hydroxycinnamic acid benzoic acid derivative benzophenone derivative beta carboline derivative carbazole derivative gentisic acid article fluorescence mass spectrometry materials membrane priority journal protein analysis technique ultraviolet spectrophotometry Erra Balsells, Rosa β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
topic_facet	β-Carbolines 9H-Pyrido[3,4-b]indoles Benzoindoles Carbazoles Phenylcarbonyl compounds Pyridoindoles UV MALDI matric UV MALDI/TOF-MS matrix acetophenone derivative alkaloid alpha cyano 4 hydroxycinnamic acid benzoic acid derivative benzophenone derivative beta carboline derivative carbazole derivative gentisic acid article fluorescence mass spectrometry materials membrane priority journal protein analysis technique ultraviolet spectrophotometry
description	The successful use of six β-carbolines (i.e. nor-harmane (9H-pyrido[3,4-b]indole), harmane (1-methyl-9H-pyrido[3,4-b]indole), harmine (7-methoxy-1-methyl-9H-pyrido[3,4-b]indole), harmol (1-methyl-9H-pyrido[3,4-b]indol-7-ol), harmaline (3,4-dihydro-7-methoxy-1-methyl-9H-pyrido[3,4-b]indole) and harmalol (3,4-dihydro-1-methyl-9H-pyrido[3,4-b]indol-7-ol)) is reported as matrices in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) (λ(exc) = 337 nm) for proteins (proteins checked: gramicidin S, bovine insulin, aprotinin, horse heart cytochrome c, ribonuclease A, lysozyme, myoglobin, trypsin, protease and bovine serum albumin) and sulfated oligosaccharides (λ-carrageenans of M(r) 549, 712, 1570 and 1733), using stainless-steel probes and membranes (poly(vinylidene difluoride) (PVDF) polymers) to prepare the samples. The possible use of some phenylcarbonyl compounds (hydroxyphenyl ketones, amino- and hydroxybenzoic acids) and a few carbazole derivatives as matrices is also discussed briefly. In addition, the usefulness of the new matrices in MALDI/TOF-MS (λ(exc) = 337 nm) of proteins and sulfated oligosaccharides is compared with those of classical MALDI matrices such as α-cyano-4-hydroxycinnamic acid, gentisic acid, sinapinic acid, 6-aza-2-thiothymine and 3-indole-trans-β-acrylic acid. Laser desorption/TOF-MS (λ(exc) = 337 nm) of the new matrices is also described.
author	Erra Balsells, Rosa
author_facet	Erra Balsells, Rosa
author_sort	Erra Balsells, Rosa
title	β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
title_short	β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
title_full	β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
title_fullStr	β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
title_full_unstemmed	β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
title_sort	β-carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: a comparative study using phenylcarbonyl compounds, carbazoles and classical matrices
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v32_n3_p287_Nonami http://hdl.handle.net/20.500.12110/paper_10765174_v32_n3_p287_Nonami
work_keys_str_mv	AT errabalsellsrosa bcarbolinealkaloidsasmatricesformatrixassistedultravioletlaserdesorptiontimeofflightmassspectrometryofproteinsandsulfatedoligosaccharidesacomparativestudyusingphenylcarbonylcompoundscarbazolesandclassicalmatrices
_version_	1768542562419736576

β-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices

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