Improved ROS measurement in root hair cells

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Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress respon...

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Autor principal:	Estevez, Jose Manuel
Publicado:	2015
Materias:	2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant root Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p67_Juarez http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p67_Juarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10643745_v1242_n_p67_Juarez
record_format	dspace
spelling	paper:paper_10643745_v1242_n_p67_Juarez2023-06-08T16:04:06Z Improved ROS measurement in root hair cells Estevez, Jose Manuel 2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241–1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2′,7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p67_Juarez http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p67_Juarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species
spellingShingle	2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species Estevez, Jose Manuel Improved ROS measurement in root hair cells
topic_facet	2′,7-Dihidro dicloro fluorescein Root hair ROS reactive oxygen metabolite 2,7-dihydrodichlorofluorescein diacetate Arabidopsis protein fluorescein derivative fluorescent dye reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase Arabidopsis thaliana Article confocal microscopy image analysis measurement nonhuman plant cell plant growth root hair Arabidopsis chemistry cytology cytosol enzymology metabolism plant cell plant root Arabidopsis Arabidopsis Proteins Cytosol Fluoresceins Fluorescent Dyes NADPH Oxidase Plant Cells Plant Roots Reactive Oxygen Species
description	Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241–1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2′,7-dihidro dicloro fluorescein (H2DCFDA). © Springer Science+Business Media New York 2015.
author	Estevez, Jose Manuel
author_facet	Estevez, Jose Manuel
author_sort	Estevez, Jose Manuel
title	Improved ROS measurement in root hair cells
title_short	Improved ROS measurement in root hair cells
title_full	Improved ROS measurement in root hair cells
title_fullStr	Improved ROS measurement in root hair cells
title_full_unstemmed	Improved ROS measurement in root hair cells
title_sort	improved ros measurement in root hair cells
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p67_Juarez http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p67_Juarez
work_keys_str_mv	AT estevezjosemanuel improvedrosmeasurementinroothaircells
_version_	1768546359458136064

Improved ROS measurement in root hair cells

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