Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae
Transient and highly regulated elevations of cytosolic Ca2+ control a variety of cellular processes. Bulk measurements using radioactive Ca2+ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells undergo a rise of cytosolic Ca2+ that is mediated by two imp...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10591524_v28_n4_p501_Carbo http://hdl.handle.net/20.500.12110/paper_10591524_v28_n4_p501_Carbo |
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paper:paper_10591524_v28_n4_p501_Carbo2025-07-30T18:39:20Z Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae calcineurin calcium fig1 protein fungal protein mid1 protein sex pheromone transcription factor transcription factor crz1 unclassified drug calcium calcium channel Fig1 protein, S cerevisiae membrane protein MID1 protein, S cerevisiae pheromone Saccharomyces cerevisiae protein sex pheromone Article cell cycle G1 phase cell cycle S phase cell growth frequency modulation fungus growth hormone response nonhuman priority journal Saccharomyces cerevisiae yeast cell cytoplasm cytosol metabolism Saccharomyces cerevisiae signal transduction Calcium Calcium Channels Cytoplasm Cytosol Membrane Glycoproteins Membrane Proteins Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sex Attractants Signal Transduction Transient and highly regulated elevations of cytosolic Ca2+ control a variety of cellular processes. Bulk measurements using radioactive Ca2+ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells undergo a rise of cytosolic Ca2+ that is mediated by two import systems composed of the Mid1-Cch1-Ecm7 protein complex and the Fig1 protein. Although this response has been widely studied, there is no treatment of Ca2+ dynamics at the single-cell level. Here, using protein calcium indicators, we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca2+ bursts. Most bursts reach maximal amplitude in 1-10 s, range between 7 and 30 s, and decay in a way that fits a single-exponential model. In vegetative cells, bursts are scarce but preferentially occur when cells are transitioning G1 and S phases. On pheromone presence, Ca2+ burst occurrence increases dramatically, persisting during cell growth polarization. Pheromone concentration modulates burst frequency in a mechanism that depends on Mid1, Fig1, and a third, unidentified, import system. We also show that the calcineurin-responsive transcription factor Crz1 undergoes nuclear localization bursts during the pheromone response. © 2017 Carbó, Tarkowski, et al. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10591524_v28_n4_p501_Carbo http://hdl.handle.net/20.500.12110/paper_10591524_v28_n4_p501_Carbo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
calcineurin calcium fig1 protein fungal protein mid1 protein sex pheromone transcription factor transcription factor crz1 unclassified drug calcium calcium channel Fig1 protein, S cerevisiae membrane protein MID1 protein, S cerevisiae pheromone Saccharomyces cerevisiae protein sex pheromone Article cell cycle G1 phase cell cycle S phase cell growth frequency modulation fungus growth hormone response nonhuman priority journal Saccharomyces cerevisiae yeast cell cytoplasm cytosol metabolism Saccharomyces cerevisiae signal transduction Calcium Calcium Channels Cytoplasm Cytosol Membrane Glycoproteins Membrane Proteins Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sex Attractants Signal Transduction |
| spellingShingle |
calcineurin calcium fig1 protein fungal protein mid1 protein sex pheromone transcription factor transcription factor crz1 unclassified drug calcium calcium channel Fig1 protein, S cerevisiae membrane protein MID1 protein, S cerevisiae pheromone Saccharomyces cerevisiae protein sex pheromone Article cell cycle G1 phase cell cycle S phase cell growth frequency modulation fungus growth hormone response nonhuman priority journal Saccharomyces cerevisiae yeast cell cytoplasm cytosol metabolism Saccharomyces cerevisiae signal transduction Calcium Calcium Channels Cytoplasm Cytosol Membrane Glycoproteins Membrane Proteins Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sex Attractants Signal Transduction Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae |
| topic_facet |
calcineurin calcium fig1 protein fungal protein mid1 protein sex pheromone transcription factor transcription factor crz1 unclassified drug calcium calcium channel Fig1 protein, S cerevisiae membrane protein MID1 protein, S cerevisiae pheromone Saccharomyces cerevisiae protein sex pheromone Article cell cycle G1 phase cell cycle S phase cell growth frequency modulation fungus growth hormone response nonhuman priority journal Saccharomyces cerevisiae yeast cell cytoplasm cytosol metabolism Saccharomyces cerevisiae signal transduction Calcium Calcium Channels Cytoplasm Cytosol Membrane Glycoproteins Membrane Proteins Pheromones Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sex Attractants Signal Transduction |
| description |
Transient and highly regulated elevations of cytosolic Ca2+ control a variety of cellular processes. Bulk measurements using radioactive Ca2+ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells undergo a rise of cytosolic Ca2+ that is mediated by two import systems composed of the Mid1-Cch1-Ecm7 protein complex and the Fig1 protein. Although this response has been widely studied, there is no treatment of Ca2+ dynamics at the single-cell level. Here, using protein calcium indicators, we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca2+ bursts. Most bursts reach maximal amplitude in 1-10 s, range between 7 and 30 s, and decay in a way that fits a single-exponential model. In vegetative cells, bursts are scarce but preferentially occur when cells are transitioning G1 and S phases. On pheromone presence, Ca2+ burst occurrence increases dramatically, persisting during cell growth polarization. Pheromone concentration modulates burst frequency in a mechanism that depends on Mid1, Fig1, and a third, unidentified, import system. We also show that the calcineurin-responsive transcription factor Crz1 undergoes nuclear localization bursts during the pheromone response. © 2017 Carbó, Tarkowski, et al. |
| title |
Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae |
| title_short |
Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae |
| title_full |
Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae |
| title_fullStr |
Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae |
| title_full_unstemmed |
Sexual pheromone modulates the frequency of cytosolic Ca2+ bursts in Saccharomyces cerevisiae |
| title_sort |
sexual pheromone modulates the frequency of cytosolic ca2+ bursts in saccharomyces cerevisiae |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10591524_v28_n4_p501_Carbo http://hdl.handle.net/20.500.12110/paper_10591524_v28_n4_p501_Carbo |
| _version_ |
1840322785926184960 |