Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies
The sequence of events during protein and lipid deposition has been examined in the developing endosperm of Myrsine laetevirens using light and electron microscopy and chemical analyses. At 10-20 d after pollination (DAP), plastids contain starch grains and phytoferritin deposits in a crystalline ar...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10585893_v160_n3_p501_Otegui http://hdl.handle.net/20.500.12110/paper_10585893_v160_n3_p501_Otegui |
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paper:paper_10585893_v160_n3_p501_Otegui2023-06-08T16:03:20Z Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies Endosperm development Lipids Myrsine laetevirens Phytoferritin Proteins Myrsine laetevirens The sequence of events during protein and lipid deposition has been examined in the developing endosperm of Myrsine laetevirens using light and electron microscopy and chemical analyses. At 10-20 d after pollination (DAP), plastids contain starch grains and phytoferritin deposits in a crystalline arrangement. Phytoferritin disappears 30 DAP, when lipid bodies are formed via secretion from smooth endoplasmic reticulum. Rough endoplasmic reticulum and Golgi stacks are present in all stages of development but are particularly evident during protein deposition. Protein bodies arise from preexistent vacuoles or derive directly from Golgi vesicles. Deposition of the crystalloid proteins occurs between 40 and 50 DAP; the matrix proteins appear at 60-70 DAP. Analysis of fatty acids by gas-liquid chromatography revealed that lipid accumulation follows a sigmoidal pattern. During the first analyzed stage (10 DAP), the fatty acid composition corresponds to structural membrane lipids. From 20 to 60 DAP, the dominant fatty acids were C18:2, C18:1, and C16:0, in a relative proportion almost similar to that of the storage lipids from mature seeds. In the mature endosperm, matrix and buffer-soluble proteins resolve into seven bands under reducing conditions and into six bands under nonreducing conditions; some of these bands were glycosylated. The crystalloid and the insoluble protein fraction resolve into five bands under both nonreducing and reducing conditions; no band is glycosylated. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10585893_v160_n3_p501_Otegui http://hdl.handle.net/20.500.12110/paper_10585893_v160_n3_p501_Otegui |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Endosperm development Lipids Myrsine laetevirens Phytoferritin Proteins Myrsine laetevirens |
| spellingShingle |
Endosperm development Lipids Myrsine laetevirens Phytoferritin Proteins Myrsine laetevirens Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies |
| topic_facet |
Endosperm development Lipids Myrsine laetevirens Phytoferritin Proteins Myrsine laetevirens |
| description |
The sequence of events during protein and lipid deposition has been examined in the developing endosperm of Myrsine laetevirens using light and electron microscopy and chemical analyses. At 10-20 d after pollination (DAP), plastids contain starch grains and phytoferritin deposits in a crystalline arrangement. Phytoferritin disappears 30 DAP, when lipid bodies are formed via secretion from smooth endoplasmic reticulum. Rough endoplasmic reticulum and Golgi stacks are present in all stages of development but are particularly evident during protein deposition. Protein bodies arise from preexistent vacuoles or derive directly from Golgi vesicles. Deposition of the crystalloid proteins occurs between 40 and 50 DAP; the matrix proteins appear at 60-70 DAP. Analysis of fatty acids by gas-liquid chromatography revealed that lipid accumulation follows a sigmoidal pattern. During the first analyzed stage (10 DAP), the fatty acid composition corresponds to structural membrane lipids. From 20 to 60 DAP, the dominant fatty acids were C18:2, C18:1, and C16:0, in a relative proportion almost similar to that of the storage lipids from mature seeds. In the mature endosperm, matrix and buffer-soluble proteins resolve into seven bands under reducing conditions and into six bands under nonreducing conditions; some of these bands were glycosylated. The crystalloid and the insoluble protein fraction resolve into five bands under both nonreducing and reducing conditions; no band is glycosylated. |
| title |
Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies |
| title_short |
Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies |
| title_full |
Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies |
| title_fullStr |
Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies |
| title_full_unstemmed |
Development of the endosperm of Myrsine laetevirens (Myrsinaceae). II. Formation of protein and lipid bodies |
| title_sort |
development of the endosperm of myrsine laetevirens (myrsinaceae). ii. formation of protein and lipid bodies |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10585893_v160_n3_p501_Otegui http://hdl.handle.net/20.500.12110/paper_10585893_v160_n3_p501_Otegui |
| _version_ |
1768545659617542144 |