Heterogeneous distribution of histone methylation in mature human sperm
Purpose: To analyze the presence of various histone modifications in ejaculated human spermatozoa. Methods: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results: We ob...
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2014
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paper:paper_10580468_v31_n1_p45_LaSpina2023-06-08T16:03:19Z Heterogeneous distribution of histone methylation in mature human sperm Epigenetic Histone modifications Human sperm Sperm subpopulations histone article cell differentiation cell structure controlled study ejaculation HeLa cell histone acetylation histone methylation histone modification human human cell immunocytochemistry immunofluorescence male normal human nucleosome priority journal prospective study protein processing semen analysis spermatid spermatozoon spermatozoon maturation Cell Nucleus Epigenesis, Genetic HeLa Cells Histone-Lysine N-Methyltransferase Histones Humans Male Methylation Semen Analysis Spermatogenesis Spermatozoa Purpose: To analyze the presence of various histone modifications in ejaculated human spermatozoa. Methods: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results: We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm. Conclusions: Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility. © 2013 Springer Science+Business Media. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10580468_v31_n1_p45_LaSpina http://hdl.handle.net/20.500.12110/paper_10580468_v31_n1_p45_LaSpina |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Epigenetic Histone modifications Human sperm Sperm subpopulations histone article cell differentiation cell structure controlled study ejaculation HeLa cell histone acetylation histone methylation histone modification human human cell immunocytochemistry immunofluorescence male normal human nucleosome priority journal prospective study protein processing semen analysis spermatid spermatozoon spermatozoon maturation Cell Nucleus Epigenesis, Genetic HeLa Cells Histone-Lysine N-Methyltransferase Histones Humans Male Methylation Semen Analysis Spermatogenesis Spermatozoa |
| spellingShingle |
Epigenetic Histone modifications Human sperm Sperm subpopulations histone article cell differentiation cell structure controlled study ejaculation HeLa cell histone acetylation histone methylation histone modification human human cell immunocytochemistry immunofluorescence male normal human nucleosome priority journal prospective study protein processing semen analysis spermatid spermatozoon spermatozoon maturation Cell Nucleus Epigenesis, Genetic HeLa Cells Histone-Lysine N-Methyltransferase Histones Humans Male Methylation Semen Analysis Spermatogenesis Spermatozoa Heterogeneous distribution of histone methylation in mature human sperm |
| topic_facet |
Epigenetic Histone modifications Human sperm Sperm subpopulations histone article cell differentiation cell structure controlled study ejaculation HeLa cell histone acetylation histone methylation histone modification human human cell immunocytochemistry immunofluorescence male normal human nucleosome priority journal prospective study protein processing semen analysis spermatid spermatozoon spermatozoon maturation Cell Nucleus Epigenesis, Genetic HeLa Cells Histone-Lysine N-Methyltransferase Histones Humans Male Methylation Semen Analysis Spermatogenesis Spermatozoa |
| description |
Purpose: To analyze the presence of various histone modifications in ejaculated human spermatozoa. Methods: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results: We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm. Conclusions: Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility. © 2013 Springer Science+Business Media. |
| title |
Heterogeneous distribution of histone methylation in mature human sperm |
| title_short |
Heterogeneous distribution of histone methylation in mature human sperm |
| title_full |
Heterogeneous distribution of histone methylation in mature human sperm |
| title_fullStr |
Heterogeneous distribution of histone methylation in mature human sperm |
| title_full_unstemmed |
Heterogeneous distribution of histone methylation in mature human sperm |
| title_sort |
heterogeneous distribution of histone methylation in mature human sperm |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10580468_v31_n1_p45_LaSpina http://hdl.handle.net/20.500.12110/paper_10580468_v31_n1_p45_LaSpina |
| _version_ |
1768544145939365888 |