Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4?
Summary: Purpose: Serum supplementation has shown to have beneficial effects on in vitro bovine embryo development. However, it is often assumed that serum supplementation may produce mitochondrial damage and this damage would generate lipid accumulation, a major obstacle for cryopreservation. The a...
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paper:paper_10580468_v30_n10_p1377_Crocco2023-06-08T16:03:18Z Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? Crocco, Melisa Candela de los Angeles Kelmansky, Diana M. Embryo Lipid droplet Mitochondria Serum Ultrastructure fat droplet article cell adhesion cell organelle cell ultrastructure controlled study cow embryo embryo development in vitro study lipid storage mitochondrial dynamics mitochondrial volume mitochondrion nonhuman priority journal serum Bovinae Animals Breeding Cattle Cryopreservation Culture Media, Serum-Free Embryo Culture Techniques Embryo, Mammalian Embryonic Development Female Lipids Mitochondria Pregnancy Reproductive Techniques, Assisted Serum Zygote Summary: Purpose: Serum supplementation has shown to have beneficial effects on in vitro bovine embryo development. However, it is often assumed that serum supplementation may produce mitochondrial damage and this damage would generate lipid accumulation, a major obstacle for cryopreservation. The aim of the present study is to investigate the previous assumptions in early embryonic stages. Methods: We considered in vitro produced bovine embryos from day 1 to 4 of development, which were grown in presence of serum from days 1, 2 or 3 or in absence of it. Electron transmission micrographs allowed us to quantify the area occupied by lipid droplets and by the different mitochondrial types to evaluate serum effect. Using confocal microscopy we analyzed mitochondrial activity and location. Results: We found no evidence of lipid droplets accumulation or mitochondrial degeneration or reduction of mitochondrial area in serum supplemented media. Further, our results suggest that events of mitochondrial proliferation are taking place even in serum supplemented media. Conclusions: Serum does not produce lipid accumulation or mitochondrial damage in bovine embryos from 2 to 16 cells. When serum was added to embryo culture medium on day 3 of development, there were ultrastructural signs of a beneficial effect for embryo development. The lack of serum until day 3 may also avoid the unnecessary exposure to potentially inhibitory factors present on it. © 2013 Springer Science+Business Media New York. Fil:Crocco, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kelmansky, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10580468_v30_n10_p1377_Crocco http://hdl.handle.net/20.500.12110/paper_10580468_v30_n10_p1377_Crocco |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Embryo Lipid droplet Mitochondria Serum Ultrastructure fat droplet article cell adhesion cell organelle cell ultrastructure controlled study cow embryo embryo development in vitro study lipid storage mitochondrial dynamics mitochondrial volume mitochondrion nonhuman priority journal serum Bovinae Animals Breeding Cattle Cryopreservation Culture Media, Serum-Free Embryo Culture Techniques Embryo, Mammalian Embryonic Development Female Lipids Mitochondria Pregnancy Reproductive Techniques, Assisted Serum Zygote |
| spellingShingle |
Embryo Lipid droplet Mitochondria Serum Ultrastructure fat droplet article cell adhesion cell organelle cell ultrastructure controlled study cow embryo embryo development in vitro study lipid storage mitochondrial dynamics mitochondrial volume mitochondrion nonhuman priority journal serum Bovinae Animals Breeding Cattle Cryopreservation Culture Media, Serum-Free Embryo Culture Techniques Embryo, Mammalian Embryonic Development Female Lipids Mitochondria Pregnancy Reproductive Techniques, Assisted Serum Zygote Crocco, Melisa Candela de los Angeles Kelmansky, Diana M. Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? |
| topic_facet |
Embryo Lipid droplet Mitochondria Serum Ultrastructure fat droplet article cell adhesion cell organelle cell ultrastructure controlled study cow embryo embryo development in vitro study lipid storage mitochondrial dynamics mitochondrial volume mitochondrion nonhuman priority journal serum Bovinae Animals Breeding Cattle Cryopreservation Culture Media, Serum-Free Embryo Culture Techniques Embryo, Mammalian Embryonic Development Female Lipids Mitochondria Pregnancy Reproductive Techniques, Assisted Serum Zygote |
| description |
Summary: Purpose: Serum supplementation has shown to have beneficial effects on in vitro bovine embryo development. However, it is often assumed that serum supplementation may produce mitochondrial damage and this damage would generate lipid accumulation, a major obstacle for cryopreservation. The aim of the present study is to investigate the previous assumptions in early embryonic stages. Methods: We considered in vitro produced bovine embryos from day 1 to 4 of development, which were grown in presence of serum from days 1, 2 or 3 or in absence of it. Electron transmission micrographs allowed us to quantify the area occupied by lipid droplets and by the different mitochondrial types to evaluate serum effect. Using confocal microscopy we analyzed mitochondrial activity and location. Results: We found no evidence of lipid droplets accumulation or mitochondrial degeneration or reduction of mitochondrial area in serum supplemented media. Further, our results suggest that events of mitochondrial proliferation are taking place even in serum supplemented media. Conclusions: Serum does not produce lipid accumulation or mitochondrial damage in bovine embryos from 2 to 16 cells. When serum was added to embryo culture medium on day 3 of development, there were ultrastructural signs of a beneficial effect for embryo development. The lack of serum until day 3 may also avoid the unnecessary exposure to potentially inhibitory factors present on it. © 2013 Springer Science+Business Media New York. |
| author |
Crocco, Melisa Candela de los Angeles Kelmansky, Diana M. |
| author_facet |
Crocco, Melisa Candela de los Angeles Kelmansky, Diana M. |
| author_sort |
Crocco, Melisa Candela de los Angeles |
| title |
Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? |
| title_short |
Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? |
| title_full |
Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? |
| title_fullStr |
Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? |
| title_full_unstemmed |
Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? |
| title_sort |
does serum cause lipid-droplet accumulation in bovine embryos produced in vitro, during developmental days 1 to 4? |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10580468_v30_n10_p1377_Crocco http://hdl.handle.net/20.500.12110/paper_10580468_v30_n10_p1377_Crocco |
| work_keys_str_mv |
AT croccomelisacandeladelosangeles doesserumcauselipiddropletaccumulationinbovineembryosproducedinvitroduringdevelopmentaldays1to4 AT kelmanskydianam doesserumcauselipiddropletaccumulationinbovineembryosproducedinvitroduringdevelopmentaldays1to4 |
| _version_ |
1768541948002435072 |