New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide
Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major v...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v28_n12_p2716_Casabuono http://hdl.handle.net/20.500.12110/paper_10440305_v28_n12_p2716_Casabuono |
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paper:paper_10440305_v28_n12_p2716_Casabuono2023-06-08T16:01:05Z New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide Brucella Lipid A Lipopolysaccharide MALDI-TOF MS Acylation Cell membranes Mass spectrometry Negative ions Brucella Core oligosaccharide Gram-negative bacteria Lipid A Lipopolysaccharides MALDI TOF MS MALDI-TOF mass spectrometry Structural studies Bacteria bacterium lipopolysaccharide disaccharide lipid A phosphate phosphoethanolamine pyrophosphorylethanolamine unclassified drug acylation Article Brucella abortus Brucella suis lipid analysis matrix assisted laser desorption ionization time of flight mass spectrometry negative ion electrospray nonhuman positive ion electrospray tandem mass spectrometry Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major virulence factor of Brucella. In the last years, structural studies allowed the determination of new structures in the core oligosaccharide and the O-antigen of this lipopolysaccharide. In this work, we have reinvestigated the lipid A structure isolated from B. suis and B. abortus lipopolysaccharides. A detailed study by MALDI-TOF mass spectrometry in the positive and negative ion modes of the lipid A moieties purified from both species was performed. Interestingly, a new feature was detected: the presence of a pyrophosphorylethanolamine residue substituting the backbone. LID-MS/MS analysis of some of the detected ions allowed assurance that the Lipid A structure composed by the diGlcN3N disaccharide, mainly hexa-acylated and penta-acylated, bearing one phosphate and one pyrophosphorylethanolamine residue. [Figure not available: see fulltext.]. © 2017, American Society for Mass Spectrometry. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v28_n12_p2716_Casabuono http://hdl.handle.net/20.500.12110/paper_10440305_v28_n12_p2716_Casabuono |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Brucella Lipid A Lipopolysaccharide MALDI-TOF MS Acylation Cell membranes Mass spectrometry Negative ions Brucella Core oligosaccharide Gram-negative bacteria Lipid A Lipopolysaccharides MALDI TOF MS MALDI-TOF mass spectrometry Structural studies Bacteria bacterium lipopolysaccharide disaccharide lipid A phosphate phosphoethanolamine pyrophosphorylethanolamine unclassified drug acylation Article Brucella abortus Brucella suis lipid analysis matrix assisted laser desorption ionization time of flight mass spectrometry negative ion electrospray nonhuman positive ion electrospray tandem mass spectrometry |
| spellingShingle |
Brucella Lipid A Lipopolysaccharide MALDI-TOF MS Acylation Cell membranes Mass spectrometry Negative ions Brucella Core oligosaccharide Gram-negative bacteria Lipid A Lipopolysaccharides MALDI TOF MS MALDI-TOF mass spectrometry Structural studies Bacteria bacterium lipopolysaccharide disaccharide lipid A phosphate phosphoethanolamine pyrophosphorylethanolamine unclassified drug acylation Article Brucella abortus Brucella suis lipid analysis matrix assisted laser desorption ionization time of flight mass spectrometry negative ion electrospray nonhuman positive ion electrospray tandem mass spectrometry New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide |
| topic_facet |
Brucella Lipid A Lipopolysaccharide MALDI-TOF MS Acylation Cell membranes Mass spectrometry Negative ions Brucella Core oligosaccharide Gram-negative bacteria Lipid A Lipopolysaccharides MALDI TOF MS MALDI-TOF mass spectrometry Structural studies Bacteria bacterium lipopolysaccharide disaccharide lipid A phosphate phosphoethanolamine pyrophosphorylethanolamine unclassified drug acylation Article Brucella abortus Brucella suis lipid analysis matrix assisted laser desorption ionization time of flight mass spectrometry negative ion electrospray nonhuman positive ion electrospray tandem mass spectrometry |
| description |
Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major virulence factor of Brucella. In the last years, structural studies allowed the determination of new structures in the core oligosaccharide and the O-antigen of this lipopolysaccharide. In this work, we have reinvestigated the lipid A structure isolated from B. suis and B. abortus lipopolysaccharides. A detailed study by MALDI-TOF mass spectrometry in the positive and negative ion modes of the lipid A moieties purified from both species was performed. Interestingly, a new feature was detected: the presence of a pyrophosphorylethanolamine residue substituting the backbone. LID-MS/MS analysis of some of the detected ions allowed assurance that the Lipid A structure composed by the diGlcN3N disaccharide, mainly hexa-acylated and penta-acylated, bearing one phosphate and one pyrophosphorylethanolamine residue. [Figure not available: see fulltext.]. © 2017, American Society for Mass Spectrometry. |
| title |
New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide |
| title_short |
New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide |
| title_full |
New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide |
| title_fullStr |
New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide |
| title_full_unstemmed |
New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide |
| title_sort |
new features in the lipid a structure of brucella suis and brucella abortus lipopolysaccharide |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v28_n12_p2716_Casabuono http://hdl.handle.net/20.500.12110/paper_10440305_v28_n12_p2716_Casabuono |
| _version_ |
1768544830126817280 |