UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different m...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v21_n1_p178_Bultel http://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_Bultel |
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paper:paper_10440305_v21_n1_p178_Bultel2023-06-08T16:01:04Z UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography Landoni, Malena Couto, Alicia Susana Kovensky, José Eduardo Controlled degradation Cyano-4-hydroxycinnamic acids Dihydroxybenzoic acids Harmane High-performance anion-exchange chromatography MALDI-TOF MALDI-TOF mass spectrometry Matrix assisted laser desorption/ionization New tools Nitrous acid Structural determination Sulfate groups Chromatographic analysis Chromatography Degradation Desorption Inorganic acids Mass spectrometry Oligosaccharides Polysaccharides Structural analysis Ultraviolet lasers Negative ions alpha cyano 4 hydroxycinnamic acid benzoic acid derivative beta carboline dihydroxybenzoic acid heparin nitrous acid oligosaccharide sulfate unclassified drug anion exchange chromatography article carbohydrate analysis chemical structure matrix assisted laser desorption ionization time of flight mass spectrometry purification structure analysis ultraviolet spectroscopy Carbohydrate Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Heparin Molecular Sequence Data Molecular Structure Nitrous Acid Oligosaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Time Factors Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry. Fil:Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v21_n1_p178_Bultel http://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_Bultel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Controlled degradation Cyano-4-hydroxycinnamic acids Dihydroxybenzoic acids Harmane High-performance anion-exchange chromatography MALDI-TOF MALDI-TOF mass spectrometry Matrix assisted laser desorption/ionization New tools Nitrous acid Structural determination Sulfate groups Chromatographic analysis Chromatography Degradation Desorption Inorganic acids Mass spectrometry Oligosaccharides Polysaccharides Structural analysis Ultraviolet lasers Negative ions alpha cyano 4 hydroxycinnamic acid benzoic acid derivative beta carboline dihydroxybenzoic acid heparin nitrous acid oligosaccharide sulfate unclassified drug anion exchange chromatography article carbohydrate analysis chemical structure matrix assisted laser desorption ionization time of flight mass spectrometry purification structure analysis ultraviolet spectroscopy Carbohydrate Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Heparin Molecular Sequence Data Molecular Structure Nitrous Acid Oligosaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Time Factors |
| spellingShingle |
Controlled degradation Cyano-4-hydroxycinnamic acids Dihydroxybenzoic acids Harmane High-performance anion-exchange chromatography MALDI-TOF MALDI-TOF mass spectrometry Matrix assisted laser desorption/ionization New tools Nitrous acid Structural determination Sulfate groups Chromatographic analysis Chromatography Degradation Desorption Inorganic acids Mass spectrometry Oligosaccharides Polysaccharides Structural analysis Ultraviolet lasers Negative ions alpha cyano 4 hydroxycinnamic acid benzoic acid derivative beta carboline dihydroxybenzoic acid heparin nitrous acid oligosaccharide sulfate unclassified drug anion exchange chromatography article carbohydrate analysis chemical structure matrix assisted laser desorption ionization time of flight mass spectrometry purification structure analysis ultraviolet spectroscopy Carbohydrate Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Heparin Molecular Sequence Data Molecular Structure Nitrous Acid Oligosaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Time Factors Landoni, Malena Couto, Alicia Susana Kovensky, José Eduardo UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
| topic_facet |
Controlled degradation Cyano-4-hydroxycinnamic acids Dihydroxybenzoic acids Harmane High-performance anion-exchange chromatography MALDI-TOF MALDI-TOF mass spectrometry Matrix assisted laser desorption/ionization New tools Nitrous acid Structural determination Sulfate groups Chromatographic analysis Chromatography Degradation Desorption Inorganic acids Mass spectrometry Oligosaccharides Polysaccharides Structural analysis Ultraviolet lasers Negative ions alpha cyano 4 hydroxycinnamic acid benzoic acid derivative beta carboline dihydroxybenzoic acid heparin nitrous acid oligosaccharide sulfate unclassified drug anion exchange chromatography article carbohydrate analysis chemical structure matrix assisted laser desorption ionization time of flight mass spectrometry purification structure analysis ultraviolet spectroscopy Carbohydrate Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Heparin Molecular Sequence Data Molecular Structure Nitrous Acid Oligosaccharides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Time Factors |
| description |
Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry. |
| author |
Landoni, Malena Couto, Alicia Susana Kovensky, José Eduardo |
| author_facet |
Landoni, Malena Couto, Alicia Susana Kovensky, José Eduardo |
| author_sort |
Landoni, Malena |
| title |
UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
| title_short |
UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
| title_full |
UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
| title_fullStr |
UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
| title_full_unstemmed |
UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
| title_sort |
uv-maldi-tof mass spectrometry analysis of heparin oligosaccharides obtained by nitrous acid controlled degradation and high performance anion exchange chromatography |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v21_n1_p178_Bultel http://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_Bultel |
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AT landonimalena uvmalditofmassspectrometryanalysisofheparinoligosaccharidesobtainedbynitrousacidcontrolleddegradationandhighperformanceanionexchangechromatography AT coutoaliciasusana uvmalditofmassspectrometryanalysisofheparinoligosaccharidesobtainedbynitrousacidcontrolleddegradationandhighperformanceanionexchangechromatography AT kovenskyjoseeduardo uvmalditofmassspectrometryanalysisofheparinoligosaccharidesobtainedbynitrousacidcontrolleddegradationandhighperformanceanionexchangechromatography |
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