Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v20_n12_p2304_Yu http://hdl.handle.net/20.500.12110/paper_10440305_v20_n12_p2304_Yu |
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paper:paper_10440305_v20_n12_p2304_Yu2023-06-08T16:01:04Z Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry Erra Balsells, Rosa Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section of a tulip bulb in different regions, including basal plate, outer and inner rims of scale, flower bud and foliage leaves. Different parts of tulip petals and leaves have also been investigated. Carbohydrates, amino acids and other phytochemicals were detected. A series of in vivo PESI-MS experiments were carried out on the second outermost scales of four living tulip bulbs to monitoring the change of carbohydrate content during the first week of initial growth. The breakdown of carbohydrates was observed which was in accordance with previous reports achieved by other techniques. This study has indicated that PESI-MS can be used for rapid and direct analysis of phytochemicals in living biological systems with advantages of low sample consumption and little sample preparation. Therefore, PESI-MS can be a new choice for direct analysis/profiling of bioactive compounds or monitoring metabolic changes in living biological systems. © 2009. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v20_n12_p2304_Yu http://hdl.handle.net/20.500.12110/paper_10440305_v20_n12_p2304_Yu |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa |
| spellingShingle |
Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa Erra Balsells, Rosa Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry |
| topic_facet |
Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa |
| description |
Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section of a tulip bulb in different regions, including basal plate, outer and inner rims of scale, flower bud and foliage leaves. Different parts of tulip petals and leaves have also been investigated. Carbohydrates, amino acids and other phytochemicals were detected. A series of in vivo PESI-MS experiments were carried out on the second outermost scales of four living tulip bulbs to monitoring the change of carbohydrate content during the first week of initial growth. The breakdown of carbohydrates was observed which was in accordance with previous reports achieved by other techniques. This study has indicated that PESI-MS can be used for rapid and direct analysis of phytochemicals in living biological systems with advantages of low sample consumption and little sample preparation. Therefore, PESI-MS can be a new choice for direct analysis/profiling of bioactive compounds or monitoring metabolic changes in living biological systems. © 2009. |
| author |
Erra Balsells, Rosa |
| author_facet |
Erra Balsells, Rosa |
| author_sort |
Erra Balsells, Rosa |
| title |
Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry |
| title_short |
Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry |
| title_full |
Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry |
| title_fullStr |
Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry |
| title_full_unstemmed |
Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry |
| title_sort |
direct profiling of phytochemicals in tulip tissues and in vivo monitoring of the change of carbohydrate content in tulip bulbs by probe electrospray ionization mass spectrometry |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10440305_v20_n12_p2304_Yu http://hdl.handle.net/20.500.12110/paper_10440305_v20_n12_p2304_Yu |
| work_keys_str_mv |
AT errabalsellsrosa directprofilingofphytochemicalsintuliptissuesandinvivomonitoringofthechangeofcarbohydratecontentintulipbulbsbyprobeelectrosprayionizationmassspectrometry |
| _version_ |
1768541856060145664 |