Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm

The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controve...

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Autores principales:	Saragüeta, Patricia, Lanuza, Guillermo
Publicado:	1994
Materias:	Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v39_n3_p280_Saragueta http://hdl.handle.net/20.500.12110/paper_1040452X_v39_n3_p280_Saragueta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1040452X_v39_n3_p280_Saragueta
record_format	dspace
spelling	paper:paper_1040452X_v39_n3_p280_Saragueta2025-07-30T18:36:41Z Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm Saragüeta, Patricia Lanuza, Guillermo Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controversial. We performed a three step purification scheme for hFF and all the fractions were screened for the AR‐inducing activity. AR activity was associated with a protein fraction of Mr > 180 kD that on further analysis under PAGE was found to be composed by subunits of apparent Mr 50,000 and 29,000. The N‐terminal sequences of these bands showed a 100% homology with the heavy and light chains of human lgG. A polyclonal antibody raised against the purified protein and anti‐human lgG were both able to suppress the acrosome reactioninducing activity of crude hFF. However, neither normal human serum nor a purified preparation of human lgG were able to mimic the AR‐inducing activity of hFF. We concluded that the AR‐inducing activity of hFF is, at least in part, due to the presence of antisperm antibodies. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc. Fil:Saragüeta, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v39_n3_p280_Saragueta http://hdl.handle.net/20.500.12110/paper_1040452X_v39_n3_p280_Saragueta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't
spellingShingle	Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't Saragüeta, Patricia Lanuza, Guillermo Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
topic_facet	Acrosome reaction Antibodies Follicular fluid Sperm immunoglobulin g immunoglobulin heavy chain immunoglobulin light chain protein subunit sperm antibody acrosome amino terminal sequence article controlled study female fertilization in vitro human human cell male ovary follicle fluid polyacrylamide gel electrophoresis priority journal sequence homology Acrosome Amino Acid Sequence Antibodies Female Follicular Fluid Human Male Molecular Sequence Data Molecular Weight Sperm Immobilizing Agents Sperm-Ovum Interactions Spermatozoa Support, Non-U.S. Gov't
description	The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controversial. We performed a three step purification scheme for hFF and all the fractions were screened for the AR‐inducing activity. AR activity was associated with a protein fraction of Mr > 180 kD that on further analysis under PAGE was found to be composed by subunits of apparent Mr 50,000 and 29,000. The N‐terminal sequences of these bands showed a 100% homology with the heavy and light chains of human lgG. A polyclonal antibody raised against the purified protein and anti‐human lgG were both able to suppress the acrosome reactioninducing activity of crude hFF. However, neither normal human serum nor a purified preparation of human lgG were able to mimic the AR‐inducing activity of hFF. We concluded that the AR‐inducing activity of hFF is, at least in part, due to the presence of antisperm antibodies. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc.
author	Saragüeta, Patricia Lanuza, Guillermo
author_facet	Saragüeta, Patricia Lanuza, Guillermo
author_sort	Saragüeta, Patricia
title	Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
title_short	Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
title_full	Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
title_fullStr	Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
title_full_unstemmed	Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
title_sort	immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm
publishDate	1994
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v39_n3_p280_Saragueta http://hdl.handle.net/20.500.12110/paper_1040452X_v39_n3_p280_Saragueta
work_keys_str_mv	AT saraguetapatricia immunoglobulinsfromhumanfollicularfluidinducetheacrosomereactioninhumansperm AT lanuzaguillermo immunoglobulinsfromhumanfollicularfluidinducetheacrosomereactioninhumansperm
_version_	1840321381304107008

Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm

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