Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins

Simple and fast methods for the monitoring of phenol-like compounds are relevant in diverse fields ranging from waste management to neurosciences. Laccases are copper-containing enzymes, which, depending on their origin, are able to oxidize different phenol compounds at different pH conditions. Thro...

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Autores principales: Wirth, Sonia Alejandra, Battaglini, Fernando
Publicado: 2017
Materias:
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v29_n2_p616_Boron
http://hdl.handle.net/20.500.12110/paper_10400397_v29_n2_p616_Boron
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spelling paper:paper_10400397_v29_n2_p616_Boron2023-06-08T16:00:40Z Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins Wirth, Sonia Alejandra Battaglini, Fernando dopamine laccase pH dependence phenol derivatives screen printed electrode Simple and fast methods for the monitoring of phenol-like compounds are relevant in diverse fields ranging from waste management to neurosciences. Laccases are copper-containing enzymes, which, depending on their origin, are able to oxidize different phenol compounds at different pH conditions. Through adequate laccase immobilization, disposable screen printed electrodes can be used as interphase to build amperometric phenol sensors. In this work three different laccases were studied for the determination of phenol-like compounds, two of them are isoenzymes from Trametes trogii and the third one from Rhus vernicifera. Their immobilization on screen printed electrodes is presented for the construction of amperometric sensors. The electrode substrate is composed by graphite screen printed electrodes modified with carbon nanotubes and silica microspheres where, depending on the application, one of the three laccases is adsorbed. As each laccase shows an optimum working pH, they were conveniently selected to determine dopamine at physiological pH and catechol at acid pH. Determinations in the micromolar range were possible in both cases. Chronoamperometry shows to be an effective technique for their determinations, simpler than other electrochemical methods already presented in the literature. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Fil:Wirth, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v29_n2_p616_Boron http://hdl.handle.net/20.500.12110/paper_10400397_v29_n2_p616_Boron
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic dopamine
laccase
pH dependence
phenol derivatives
screen printed electrode
spellingShingle dopamine
laccase
pH dependence
phenol derivatives
screen printed electrode
Wirth, Sonia Alejandra
Battaglini, Fernando
Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins
topic_facet dopamine
laccase
pH dependence
phenol derivatives
screen printed electrode
description Simple and fast methods for the monitoring of phenol-like compounds are relevant in diverse fields ranging from waste management to neurosciences. Laccases are copper-containing enzymes, which, depending on their origin, are able to oxidize different phenol compounds at different pH conditions. Through adequate laccase immobilization, disposable screen printed electrodes can be used as interphase to build amperometric phenol sensors. In this work three different laccases were studied for the determination of phenol-like compounds, two of them are isoenzymes from Trametes trogii and the third one from Rhus vernicifera. Their immobilization on screen printed electrodes is presented for the construction of amperometric sensors. The electrode substrate is composed by graphite screen printed electrodes modified with carbon nanotubes and silica microspheres where, depending on the application, one of the three laccases is adsorbed. As each laccase shows an optimum working pH, they were conveniently selected to determine dopamine at physiological pH and catechol at acid pH. Determinations in the micromolar range were possible in both cases. Chronoamperometry shows to be an effective technique for their determinations, simpler than other electrochemical methods already presented in the literature. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
author Wirth, Sonia Alejandra
Battaglini, Fernando
author_facet Wirth, Sonia Alejandra
Battaglini, Fernando
author_sort Wirth, Sonia Alejandra
title Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins
title_short Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins
title_full Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins
title_fullStr Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins
title_full_unstemmed Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins
title_sort versatile electrochemical platform for the determination of phenol-like compounds based on laccases from different origins
publishDate 2017
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v29_n2_p616_Boron
http://hdl.handle.net/20.500.12110/paper_10400397_v29_n2_p616_Boron
work_keys_str_mv AT wirthsoniaalejandra versatileelectrochemicalplatformforthedeterminationofphenollikecompoundsbasedonlaccasesfromdifferentorigins
AT battaglinifernando versatileelectrochemicalplatformforthedeterminationofphenollikecompoundsbasedonlaccasesfromdifferentorigins
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