Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins
Simple and fast methods for the monitoring of phenol-like compounds are relevant in diverse fields ranging from waste management to neurosciences. Laccases are copper-containing enzymes, which, depending on their origin, are able to oxidize different phenol compounds at different pH conditions. Thro...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v29_n2_p616_Boron http://hdl.handle.net/20.500.12110/paper_10400397_v29_n2_p616_Boron |
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paper:paper_10400397_v29_n2_p616_Boron2023-06-08T16:00:40Z Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins Wirth, Sonia Alejandra Battaglini, Fernando dopamine laccase pH dependence phenol derivatives screen printed electrode Simple and fast methods for the monitoring of phenol-like compounds are relevant in diverse fields ranging from waste management to neurosciences. Laccases are copper-containing enzymes, which, depending on their origin, are able to oxidize different phenol compounds at different pH conditions. Through adequate laccase immobilization, disposable screen printed electrodes can be used as interphase to build amperometric phenol sensors. In this work three different laccases were studied for the determination of phenol-like compounds, two of them are isoenzymes from Trametes trogii and the third one from Rhus vernicifera. Their immobilization on screen printed electrodes is presented for the construction of amperometric sensors. The electrode substrate is composed by graphite screen printed electrodes modified with carbon nanotubes and silica microspheres where, depending on the application, one of the three laccases is adsorbed. As each laccase shows an optimum working pH, they were conveniently selected to determine dopamine at physiological pH and catechol at acid pH. Determinations in the micromolar range were possible in both cases. Chronoamperometry shows to be an effective technique for their determinations, simpler than other electrochemical methods already presented in the literature. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Fil:Wirth, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v29_n2_p616_Boron http://hdl.handle.net/20.500.12110/paper_10400397_v29_n2_p616_Boron |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
dopamine laccase pH dependence phenol derivatives screen printed electrode |
spellingShingle |
dopamine laccase pH dependence phenol derivatives screen printed electrode Wirth, Sonia Alejandra Battaglini, Fernando Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins |
topic_facet |
dopamine laccase pH dependence phenol derivatives screen printed electrode |
description |
Simple and fast methods for the monitoring of phenol-like compounds are relevant in diverse fields ranging from waste management to neurosciences. Laccases are copper-containing enzymes, which, depending on their origin, are able to oxidize different phenol compounds at different pH conditions. Through adequate laccase immobilization, disposable screen printed electrodes can be used as interphase to build amperometric phenol sensors. In this work three different laccases were studied for the determination of phenol-like compounds, two of them are isoenzymes from Trametes trogii and the third one from Rhus vernicifera. Their immobilization on screen printed electrodes is presented for the construction of amperometric sensors. The electrode substrate is composed by graphite screen printed electrodes modified with carbon nanotubes and silica microspheres where, depending on the application, one of the three laccases is adsorbed. As each laccase shows an optimum working pH, they were conveniently selected to determine dopamine at physiological pH and catechol at acid pH. Determinations in the micromolar range were possible in both cases. Chronoamperometry shows to be an effective technique for their determinations, simpler than other electrochemical methods already presented in the literature. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
author |
Wirth, Sonia Alejandra Battaglini, Fernando |
author_facet |
Wirth, Sonia Alejandra Battaglini, Fernando |
author_sort |
Wirth, Sonia Alejandra |
title |
Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins |
title_short |
Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins |
title_full |
Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins |
title_fullStr |
Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins |
title_full_unstemmed |
Versatile Electrochemical Platform for the Determination of Phenol-like Compounds Based on Laccases from Different Origins |
title_sort |
versatile electrochemical platform for the determination of phenol-like compounds based on laccases from different origins |
publishDate |
2017 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v29_n2_p616_Boron http://hdl.handle.net/20.500.12110/paper_10400397_v29_n2_p616_Boron |
work_keys_str_mv |
AT wirthsoniaalejandra versatileelectrochemicalplatformforthedeterminationofphenollikecompoundsbasedonlaccasesfromdifferentorigins AT battaglinifernando versatileelectrochemicalplatformforthedeterminationofphenollikecompoundsbasedonlaccasesfromdifferentorigins |
_version_ |
1768546638257717248 |