An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells

Whole cells of the yeast Rhodotorula minuta were used in the biotransformation of dialkyl esters of 2-oxoglutaric acid. Almost 100% of conversion with 97-98% of enantiomeric excess of the (S) form of 2-hydroxydiesters was obtained through an enantioselective reduction of dimethyl and diethyl 2-oxogl...

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Autores principales:	Rustoy, Eduardo Miguel, Cerrutti, Patricia, Galvagno, Miguel Angel, Baldessari, Alicia
Publicado:	2008
Materias:	Biotransformation Dialkyl 2-oxoglutaric acid esters Rhodotorula minuta Cells Enantiomers Enantioselectivity Yeast Dialkyl esters Oxoglutaric acid Esters 2 oxoglutaric acid 4 hydroxybutyric acid article biocatalyst biotransformation decarboxylation enantiomer enantioselectivity hydrolysis nonhuman reduction Rhodotorula
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10242422_v26_n3_p204_Rustoy http://hdl.handle.net/20.500.12110/paper_10242422_v26_n3_p204_Rustoy
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10242422_v26_n3_p204_Rustoy
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spelling	paper:paper_10242422_v26_n3_p204_Rustoy2023-06-08T16:00:12Z An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells Rustoy, Eduardo Miguel Cerrutti, Patricia Galvagno, Miguel Angel Baldessari, Alicia Biotransformation Dialkyl 2-oxoglutaric acid esters Rhodotorula minuta Cells Enantiomers Enantioselectivity Yeast Biotransformation Dialkyl esters Oxoglutaric acid Esters 2 oxoglutaric acid 4 hydroxybutyric acid article biocatalyst biotransformation decarboxylation enantiomer enantioselectivity hydrolysis nonhuman reduction Rhodotorula Rhodotorula minuta Rhodotorula minuta Whole cells of the yeast Rhodotorula minuta were used in the biotransformation of dialkyl esters of 2-oxoglutaric acid. Almost 100% of conversion with 97-98% of enantiomeric excess of the (S) form of 2-hydroxydiesters was obtained through an enantioselective reduction of dimethyl and diethyl 2-oxoglutarate. When longer alkoxy chain 2-oxoglutarates were used as substrates, the corresponding 4-hydroxybutyric esters were obtained, suggesting a combination process including hydrolysis, decarboxylation and reduction. The cells showed a remarkable high productivity: high conversion and enantiomeric excess were obtained at 2 g wet weight mmol-1 substrate. Fil:Rustoy, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerrutti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baldessari, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10242422_v26_n3_p204_Rustoy http://hdl.handle.net/20.500.12110/paper_10242422_v26_n3_p204_Rustoy
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biotransformation Dialkyl 2-oxoglutaric acid esters Rhodotorula minuta Cells Enantiomers Enantioselectivity Yeast Biotransformation Dialkyl esters Oxoglutaric acid Esters 2 oxoglutaric acid 4 hydroxybutyric acid article biocatalyst biotransformation decarboxylation enantiomer enantioselectivity hydrolysis nonhuman reduction Rhodotorula Rhodotorula minuta Rhodotorula minuta
spellingShingle	Biotransformation Dialkyl 2-oxoglutaric acid esters Rhodotorula minuta Cells Enantiomers Enantioselectivity Yeast Biotransformation Dialkyl esters Oxoglutaric acid Esters 2 oxoglutaric acid 4 hydroxybutyric acid article biocatalyst biotransformation decarboxylation enantiomer enantioselectivity hydrolysis nonhuman reduction Rhodotorula Rhodotorula minuta Rhodotorula minuta Rustoy, Eduardo Miguel Cerrutti, Patricia Galvagno, Miguel Angel Baldessari, Alicia An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
topic_facet	Biotransformation Dialkyl 2-oxoglutaric acid esters Rhodotorula minuta Cells Enantiomers Enantioselectivity Yeast Biotransformation Dialkyl esters Oxoglutaric acid Esters 2 oxoglutaric acid 4 hydroxybutyric acid article biocatalyst biotransformation decarboxylation enantiomer enantioselectivity hydrolysis nonhuman reduction Rhodotorula Rhodotorula minuta Rhodotorula minuta
description	Whole cells of the yeast Rhodotorula minuta were used in the biotransformation of dialkyl esters of 2-oxoglutaric acid. Almost 100% of conversion with 97-98% of enantiomeric excess of the (S) form of 2-hydroxydiesters was obtained through an enantioselective reduction of dimethyl and diethyl 2-oxoglutarate. When longer alkoxy chain 2-oxoglutarates were used as substrates, the corresponding 4-hydroxybutyric esters were obtained, suggesting a combination process including hydrolysis, decarboxylation and reduction. The cells showed a remarkable high productivity: high conversion and enantiomeric excess were obtained at 2 g wet weight mmol-1 substrate.
author	Rustoy, Eduardo Miguel Cerrutti, Patricia Galvagno, Miguel Angel Baldessari, Alicia
author_facet	Rustoy, Eduardo Miguel Cerrutti, Patricia Galvagno, Miguel Angel Baldessari, Alicia
author_sort	Rustoy, Eduardo Miguel
title	An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
title_short	An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
title_full	An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
title_fullStr	An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
title_full_unstemmed	An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
title_sort	efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by rhodotorula minuta whole cells
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10242422_v26_n3_p204_Rustoy http://hdl.handle.net/20.500.12110/paper_10242422_v26_n3_p204_Rustoy
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An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells

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