Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement
Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest y...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10226680_v770_n_p402_Leasen http://hdl.handle.net/20.500.12110/paper_10226680_v770_n_p402_Leasen |
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paper:paper_10226680_v770_n_p402_Leasen2023-06-08T16:00:08Z Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement 3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene blue Tin-doped indium oxide 3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene Blue Tin doped indium oxide Aromatic compounds Cyclic voltammetry DNA DNA sequences Organic polymers Oxide films Probes Self assembled monolayers Tin Polymerase chain reaction Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events. © (2013) Trans Tech Publications, Switzerland. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10226680_v770_n_p402_Leasen http://hdl.handle.net/20.500.12110/paper_10226680_v770_n_p402_Leasen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene blue Tin-doped indium oxide 3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene Blue Tin doped indium oxide Aromatic compounds Cyclic voltammetry DNA DNA sequences Organic polymers Oxide films Probes Self assembled monolayers Tin Polymerase chain reaction |
| spellingShingle |
3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene blue Tin-doped indium oxide 3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene Blue Tin doped indium oxide Aromatic compounds Cyclic voltammetry DNA DNA sequences Organic polymers Oxide films Probes Self assembled monolayers Tin Polymerase chain reaction Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| topic_facet |
3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene blue Tin-doped indium oxide 3-mercaptopropionic acid Differential pulse voltammetry Hybridization Methylene Blue Tin doped indium oxide Aromatic compounds Cyclic voltammetry DNA DNA sequences Organic polymers Oxide films Probes Self assembled monolayers Tin Polymerase chain reaction |
| description |
Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events. © (2013) Trans Tech Publications, Switzerland. |
| title |
Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| title_short |
Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| title_full |
Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| title_fullStr |
Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| title_full_unstemmed |
Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| title_sort |
detecting dna-dna hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10226680_v770_n_p402_Leasen http://hdl.handle.net/20.500.12110/paper_10226680_v770_n_p402_Leasen |
| _version_ |
1768544236807913472 |