Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
We have previously described the regulation of interleukin-1 receptor antagonist (IL-1ra) protein secretion and expression by IL-1, glucocorticoids and corticotropin-releasing hormone in monocytes in culture. In the present work, we analyze the direct effect of adrenocorticotropic hormone (ACTH) and...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v6_n5_p367_Kovalovsky http://hdl.handle.net/20.500.12110/paper_10217401_v6_n5_p367_Kovalovsky |
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paper:paper_10217401_v6_n5_p367_Kovalovsky2023-06-08T16:00:02Z Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin Kovalovsky, Damián Páez Pereda, Marcelo β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins We have previously described the regulation of interleukin-1 receptor antagonist (IL-1ra) protein secretion and expression by IL-1, glucocorticoids and corticotropin-releasing hormone in monocytes in culture. In the present work, we analyze the direct effect of adrenocorticotropic hormone (ACTH) and β-endorphin on the expression and secretion of IL-1ra by human monocytes in culture. ACTH exerted a dose-dependent inhibitory effect on lipopolysaccharide (LPS)-induced IL-1ra production and mRNA expression. Basal IL-1ra levels were not affected by treatment with any ACTH dose. In contrast, on human monocytes, β-endorphin at concentrations as low as 10 pg/ml produced an increase of basal IL-1ra protein secretion and mRNA expression, this effect being reverted by pretreatment with naloxone. No effect of β- endorphin was observed either in IL-1ra mRNA expression or protein secretion when cells were treated with LPS. The different effects of ACTH and β- endorphin could account for their differential contribution to the inflammatory response: while ACTH contributes to the glucocorticoid overall control of the inflammatory response, β-endorphin exerts an inhibitory tone on the resting IL-1 system. Because IL-1ra is essential in setting the level of monocyte and inflammatory response its differential regulation by the HPA axis hormones contributes to regulating the IL-1/inflammatory temporal response. Fil:Kovalovsky, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v6_n5_p367_Kovalovsky http://hdl.handle.net/20.500.12110/paper_10217401_v6_n5_p367_Kovalovsky |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins |
| spellingShingle |
β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins Kovalovsky, Damián Páez Pereda, Marcelo Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
| topic_facet |
β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins |
| description |
We have previously described the regulation of interleukin-1 receptor antagonist (IL-1ra) protein secretion and expression by IL-1, glucocorticoids and corticotropin-releasing hormone in monocytes in culture. In the present work, we analyze the direct effect of adrenocorticotropic hormone (ACTH) and β-endorphin on the expression and secretion of IL-1ra by human monocytes in culture. ACTH exerted a dose-dependent inhibitory effect on lipopolysaccharide (LPS)-induced IL-1ra production and mRNA expression. Basal IL-1ra levels were not affected by treatment with any ACTH dose. In contrast, on human monocytes, β-endorphin at concentrations as low as 10 pg/ml produced an increase of basal IL-1ra protein secretion and mRNA expression, this effect being reverted by pretreatment with naloxone. No effect of β- endorphin was observed either in IL-1ra mRNA expression or protein secretion when cells were treated with LPS. The different effects of ACTH and β- endorphin could account for their differential contribution to the inflammatory response: while ACTH contributes to the glucocorticoid overall control of the inflammatory response, β-endorphin exerts an inhibitory tone on the resting IL-1 system. Because IL-1ra is essential in setting the level of monocyte and inflammatory response its differential regulation by the HPA axis hormones contributes to regulating the IL-1/inflammatory temporal response. |
| author |
Kovalovsky, Damián Páez Pereda, Marcelo |
| author_facet |
Kovalovsky, Damián Páez Pereda, Marcelo |
| author_sort |
Kovalovsky, Damián |
| title |
Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
| title_short |
Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
| title_full |
Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
| title_fullStr |
Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
| title_full_unstemmed |
Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
| title_sort |
differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v6_n5_p367_Kovalovsky http://hdl.handle.net/20.500.12110/paper_10217401_v6_n5_p367_Kovalovsky |
| work_keys_str_mv |
AT kovalovskydamian differentialregulationofinterleukin1receptorantagonistbyproopiomelanocortinpeptidesadrenocorticotropichormoneandbendorphin AT paezperedamarcelo differentialregulationofinterleukin1receptorantagonistbyproopiomelanocortinpeptidesadrenocorticotropichormoneandbendorphin |
| _version_ |
1768542798547517440 |