Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential
In the present work we used a murine mammary cancer model of two related adenocarcinomas with different lung metastasizing abilities, to compare their global gene expression profiles. Clontech Atlas mouse cDNA microarrays of primary cultured tumor cells were employed to identify genes that are modul...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1021335X_v16_n6_p1403_Urtreger http://hdl.handle.net/20.500.12110/paper_1021335X_v16_n6_p1403_Urtreger |
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paper:paper_1021335X_v16_n6_p1403_Urtreger2025-07-30T18:35:57Z Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential cDNA arrays Fibronectin Metastasizing ability Murine mammary adenocarcinoma fibronectin adenocarcinoma animal article comparative study DNA microarray down regulation experimental neoplasm female gene expression gene expression profiling gene expression regulation genetics metabolism metastasis mouse pathology Western blotting Adenocarcinoma Animals Blotting, Western Down-Regulation Female Fibronectins Gene Expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Mammary Neoplasms, Experimental Mice Neoplasm Metastasis Oligonucleotide Array Sequence Analysis In the present work we used a murine mammary cancer model of two related adenocarcinomas with different lung metastasizing abilities, to compare their global gene expression profiles. Clontech Atlas mouse cDNA microarrays of primary cultured tumor cells were employed to identify genes that are modulated in the more metastatic variant MM3 relative to its parental tumor M3. A total of 88 from 1,176 genes were differentially expressed in MM3 primary cultures, most of them (n=86) were upregulated. Genes were grouped according to their functions as associated with signal transduction and transcription regulation (e.g. Stat1 and Zfp 92), with cell adhesion and motility (cadherin 1, fibronectin), with invasion and angiogenesis (uPA, 72 kDa MMP2), with the regulation of cell proliferation and cell death (cyclins G and A2, TNF), and also included growth factors and receptors, oncogenes and tumor suppressors genes (p107, TGFβ2, TBR-I, PDGFR). Only 2 genes, TTF1 and fibronectin (FN), showed a significant downregulation. Notably FN expression, loss of which has been associated with a malignant phenotype, was reduced about 19-fold in the more metastatic MM3 cells. Previously known differences in expression patterns associated with the metastatic capacity of MM3 and M3 adenocarcinomas, including downregulation of FN or upregulated expression of TGFβ and proteases, were confirmed by the array data. The fact that FN was one of the only two genes significantly down-regulated out of the 1,176 genes analyzed stresses the hypothesis that FN may behave as an important metastasis suppressor gene in mammary cancer. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1021335X_v16_n6_p1403_Urtreger http://hdl.handle.net/20.500.12110/paper_1021335X_v16_n6_p1403_Urtreger |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cDNA arrays Fibronectin Metastasizing ability Murine mammary adenocarcinoma fibronectin adenocarcinoma animal article comparative study DNA microarray down regulation experimental neoplasm female gene expression gene expression profiling gene expression regulation genetics metabolism metastasis mouse pathology Western blotting Adenocarcinoma Animals Blotting, Western Down-Regulation Female Fibronectins Gene Expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Mammary Neoplasms, Experimental Mice Neoplasm Metastasis Oligonucleotide Array Sequence Analysis |
| spellingShingle |
cDNA arrays Fibronectin Metastasizing ability Murine mammary adenocarcinoma fibronectin adenocarcinoma animal article comparative study DNA microarray down regulation experimental neoplasm female gene expression gene expression profiling gene expression regulation genetics metabolism metastasis mouse pathology Western blotting Adenocarcinoma Animals Blotting, Western Down-Regulation Female Fibronectins Gene Expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Mammary Neoplasms, Experimental Mice Neoplasm Metastasis Oligonucleotide Array Sequence Analysis Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential |
| topic_facet |
cDNA arrays Fibronectin Metastasizing ability Murine mammary adenocarcinoma fibronectin adenocarcinoma animal article comparative study DNA microarray down regulation experimental neoplasm female gene expression gene expression profiling gene expression regulation genetics metabolism metastasis mouse pathology Western blotting Adenocarcinoma Animals Blotting, Western Down-Regulation Female Fibronectins Gene Expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Mammary Neoplasms, Experimental Mice Neoplasm Metastasis Oligonucleotide Array Sequence Analysis |
| description |
In the present work we used a murine mammary cancer model of two related adenocarcinomas with different lung metastasizing abilities, to compare their global gene expression profiles. Clontech Atlas mouse cDNA microarrays of primary cultured tumor cells were employed to identify genes that are modulated in the more metastatic variant MM3 relative to its parental tumor M3. A total of 88 from 1,176 genes were differentially expressed in MM3 primary cultures, most of them (n=86) were upregulated. Genes were grouped according to their functions as associated with signal transduction and transcription regulation (e.g. Stat1 and Zfp 92), with cell adhesion and motility (cadherin 1, fibronectin), with invasion and angiogenesis (uPA, 72 kDa MMP2), with the regulation of cell proliferation and cell death (cyclins G and A2, TNF), and also included growth factors and receptors, oncogenes and tumor suppressors genes (p107, TGFβ2, TBR-I, PDGFR). Only 2 genes, TTF1 and fibronectin (FN), showed a significant downregulation. Notably FN expression, loss of which has been associated with a malignant phenotype, was reduced about 19-fold in the more metastatic MM3 cells. Previously known differences in expression patterns associated with the metastatic capacity of MM3 and M3 adenocarcinomas, including downregulation of FN or upregulated expression of TGFβ and proteases, were confirmed by the array data. The fact that FN was one of the only two genes significantly down-regulated out of the 1,176 genes analyzed stresses the hypothesis that FN may behave as an important metastasis suppressor gene in mammary cancer. |
| title |
Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential |
| title_short |
Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential |
| title_full |
Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential |
| title_fullStr |
Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential |
| title_full_unstemmed |
Fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential |
| title_sort |
fibronectin is distinctly downregulated in murine mammary adenocarcinoma cells with high metastatic potential |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1021335X_v16_n6_p1403_Urtreger http://hdl.handle.net/20.500.12110/paper_1021335X_v16_n6_p1403_Urtreger |
| _version_ |
1840326350211121152 |