Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice

Mast cells (MC) are frequently increased in neoplasias. Recently, we observed that MC from peritoneal cavity of normal mice (NMC) and one of their mediators (heparin) decreased M3 tumor incidence and tumor cell proliferation in vitro, while MC from peritoneal cavity of tumor-bearing mice (TMC) had n...

Descripción completa

Detalles Bibliográficos
Publicado: 1997
Materias:
Adenocarcinoma
Heparin
Image analysis
Mast cell
Tris (2,2'-bipyridine) ruthenium (II)
adenocarcinoma
animal cell
article
breast carcinoma
image analysis
mast cell degranulation
microfluorometry
mouse
nonhuman
peritoneum mast cell
priority journal
tumor growth
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi
http://hdl.handle.net/20.500.12110/paper_10196439_v11_n6_p1221_Bertolesi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_10196439_v11_n6_p1221_Bertolesi
record_format dspace
spelling paper:paper_10196439_v11_n6_p1221_Bertolesi2023-06-08T15:59:54Z Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice Adenocarcinoma Heparin Image analysis Mast cell Tris (2,2'-bipyridine) ruthenium (II) adenocarcinoma animal cell article breast carcinoma image analysis mast cell degranulation microfluorometry mouse nonhuman peritoneum mast cell priority journal tumor growth Mast cells (MC) are frequently increased in neoplasias. Recently, we observed that MC from peritoneal cavity of normal mice (NMC) and one of their mediators (heparin) decreased M3 tumor incidence and tumor cell proliferation in vitro, while MC from peritoneal cavity of tumor-bearing mice (TMC) had no effect. The purpose of this study was to evaluate the differences in morphology and content of sulfated glycosaminoglycans between NMC and TMC. Both were stained with Mayer's haematoxylin-Rubipy [tris (2,2'-bipyridine) ruthenium (II)] sequence, a specific technique to detect sulfated polysaccharides. Image processing and analysis (IPA) confirmed densitometric and microfluorometric studies and revealed several structural characteristics of MC. TMC were partially degranulated with smaller surface and greater perimeter than NMC. Shape factor, which reflects the sphericity grade of the cell (1 = spherical), was three-fold increased in TMC in relation to NMC (6.15 vs 1.76, respectively). Also, TMC had less than a half sulfated polysaccharides compared to NMC. We conclude that subcutaneous tumor grafts mediate degranulation of MC from peritoneal cavity with the consequent release of MC mediators such as heparin. This may be one of the factors for the absence of antitumoral effect of TMC. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi http://hdl.handle.net/20.500.12110/paper_10196439_v11_n6_p1221_Bertolesi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adenocarcinoma
Heparin
Image analysis
Mast cell
Tris (2,2'-bipyridine) ruthenium (II)
adenocarcinoma
animal cell
article
breast carcinoma
image analysis
mast cell degranulation
microfluorometry
mouse
nonhuman
peritoneum mast cell
priority journal
tumor growth
spellingShingle Adenocarcinoma
Heparin
Image analysis
Mast cell
Tris (2,2'-bipyridine) ruthenium (II)
adenocarcinoma
animal cell
article
breast carcinoma
image analysis
mast cell degranulation
microfluorometry
mouse
nonhuman
peritoneum mast cell
priority journal
tumor growth
Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice
topic_facet Adenocarcinoma
Heparin
Image analysis
Mast cell
Tris (2,2'-bipyridine) ruthenium (II)
adenocarcinoma
animal cell
article
breast carcinoma
image analysis
mast cell degranulation
microfluorometry
mouse
nonhuman
peritoneum mast cell
priority journal
tumor growth
description Mast cells (MC) are frequently increased in neoplasias. Recently, we observed that MC from peritoneal cavity of normal mice (NMC) and one of their mediators (heparin) decreased M3 tumor incidence and tumor cell proliferation in vitro, while MC from peritoneal cavity of tumor-bearing mice (TMC) had no effect. The purpose of this study was to evaluate the differences in morphology and content of sulfated glycosaminoglycans between NMC and TMC. Both were stained with Mayer's haematoxylin-Rubipy [tris (2,2'-bipyridine) ruthenium (II)] sequence, a specific technique to detect sulfated polysaccharides. Image processing and analysis (IPA) confirmed densitometric and microfluorometric studies and revealed several structural characteristics of MC. TMC were partially degranulated with smaller surface and greater perimeter than NMC. Shape factor, which reflects the sphericity grade of the cell (1 = spherical), was three-fold increased in TMC in relation to NMC (6.15 vs 1.76, respectively). Also, TMC had less than a half sulfated polysaccharides compared to NMC. We conclude that subcutaneous tumor grafts mediate degranulation of MC from peritoneal cavity with the consequent release of MC mediators such as heparin. This may be one of the factors for the absence of antitumoral effect of TMC.
title Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice
title_short Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice
title_full Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice
title_fullStr Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice
title_full_unstemmed Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice
title_sort microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice
publishDate 1997
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi
http://hdl.handle.net/20.500.12110/paper_10196439_v11_n6_p1221_Bertolesi
_version_ 1768543382762684416

Ejemplares similares