Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes
Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v33_n6_p1745_Cacace http://hdl.handle.net/20.500.12110/paper_10158987_v33_n6_p1745_Cacace |
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paper:paper_10158987_v33_n6_p1745_Cacace2023-06-08T15:59:47Z Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes Bumetanide Cell volume regulation DIDS HL-1 atrial myocytes RVD RVI 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide hypertonic solution hypotonic solution sodium potassium chloride cotransporter bicarbonate chloride antiporter isotonic solution Ringer solution sodium potassium chloride cotransporter 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide diuretic agent hypertonic solution hypotonic solution animal cell animal experiment article biological activity cell function cell membrane transport cell swelling cell volume cellular parameters concentration response enzyme inhibition heart muscle cell hyperosmotic stress hypoosmotic stress light intensity light scattering nonhuman osmosis osmotic stress priority journal regulatory mechanism regulatory volume decrease regulatory volume increase Article cell membrane controlled study illumination mouse water flow animal cardiac muscle cell cell line cell size cytology drug effects time factor 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid Animals Bumetanide Cell Line Cell Size Diuretics Hypertonic Solutions Hypotonic Solutions Mice Myocytes, Cardiac Osmosis Time Factors Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in volume regulation of HL-1 cells. Methods: we used a light scattering system to measure the transient volume changes of HL-1 cells when subjected to osmotic challenge. Results: We found that HL-1 cells correct for their volume excess by undergoing regulatory volume decrease (RVD), and also respond to hypertonic stress with a regulatory volume increase (RVI). Rate of RVD was 0.08 ± 0.04 intensity/min, and rate of RVI was 0.09 ± 0.01 intensity/min. Volume recovery was 83.68 ± 5.73 % for RVD and 92.3 ± 2.3 % for RVI. Bumetanide 50 μM inhibited volume recovery, from 92.3 ± 2.3 % (control) to 24.6 ± 8.8 % and reduced the rate of RVI from 0.070 ± 0.020 intensity/min (control) to 0.010 ± 0.005 intensity/min. 50 μM DIDS reduced volume recovery to 42.93 ± 7.7 % and rate of RVI, to 0.03 ± 0.01 intensity/min. Conclusions: these results suggest that bumetanide- and DIDS-sensitive mechanisms are involved in the RVI of HL-1 cells, which points to the involvement of the Na+/K+/2Cl- cotransporter and Cl-/bicarbonate exchanger in RVI, respectively. © 2014 S. Karger AG, Basel. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v33_n6_p1745_Cacace http://hdl.handle.net/20.500.12110/paper_10158987_v33_n6_p1745_Cacace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bumetanide Cell volume regulation DIDS HL-1 atrial myocytes RVD RVI 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide hypertonic solution hypotonic solution sodium potassium chloride cotransporter bicarbonate chloride antiporter isotonic solution Ringer solution sodium potassium chloride cotransporter 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide diuretic agent hypertonic solution hypotonic solution animal cell animal experiment article biological activity cell function cell membrane transport cell swelling cell volume cellular parameters concentration response enzyme inhibition heart muscle cell hyperosmotic stress hypoosmotic stress light intensity light scattering nonhuman osmosis osmotic stress priority journal regulatory mechanism regulatory volume decrease regulatory volume increase Article cell membrane controlled study illumination mouse water flow animal cardiac muscle cell cell line cell size cytology drug effects time factor 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid Animals Bumetanide Cell Line Cell Size Diuretics Hypertonic Solutions Hypotonic Solutions Mice Myocytes, Cardiac Osmosis Time Factors |
| spellingShingle |
Bumetanide Cell volume regulation DIDS HL-1 atrial myocytes RVD RVI 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide hypertonic solution hypotonic solution sodium potassium chloride cotransporter bicarbonate chloride antiporter isotonic solution Ringer solution sodium potassium chloride cotransporter 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide diuretic agent hypertonic solution hypotonic solution animal cell animal experiment article biological activity cell function cell membrane transport cell swelling cell volume cellular parameters concentration response enzyme inhibition heart muscle cell hyperosmotic stress hypoosmotic stress light intensity light scattering nonhuman osmosis osmotic stress priority journal regulatory mechanism regulatory volume decrease regulatory volume increase Article cell membrane controlled study illumination mouse water flow animal cardiac muscle cell cell line cell size cytology drug effects time factor 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid Animals Bumetanide Cell Line Cell Size Diuretics Hypertonic Solutions Hypotonic Solutions Mice Myocytes, Cardiac Osmosis Time Factors Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes |
| topic_facet |
Bumetanide Cell volume regulation DIDS HL-1 atrial myocytes RVD RVI 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide hypertonic solution hypotonic solution sodium potassium chloride cotransporter bicarbonate chloride antiporter isotonic solution Ringer solution sodium potassium chloride cotransporter 4,4' diisothiocyanatostilbene 2,2' disulfonic acid bumetanide diuretic agent hypertonic solution hypotonic solution animal cell animal experiment article biological activity cell function cell membrane transport cell swelling cell volume cellular parameters concentration response enzyme inhibition heart muscle cell hyperosmotic stress hypoosmotic stress light intensity light scattering nonhuman osmosis osmotic stress priority journal regulatory mechanism regulatory volume decrease regulatory volume increase Article cell membrane controlled study illumination mouse water flow animal cardiac muscle cell cell line cell size cytology drug effects time factor 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid Animals Bumetanide Cell Line Cell Size Diuretics Hypertonic Solutions Hypotonic Solutions Mice Myocytes, Cardiac Osmosis Time Factors |
| description |
Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in volume regulation of HL-1 cells. Methods: we used a light scattering system to measure the transient volume changes of HL-1 cells when subjected to osmotic challenge. Results: We found that HL-1 cells correct for their volume excess by undergoing regulatory volume decrease (RVD), and also respond to hypertonic stress with a regulatory volume increase (RVI). Rate of RVD was 0.08 ± 0.04 intensity/min, and rate of RVI was 0.09 ± 0.01 intensity/min. Volume recovery was 83.68 ± 5.73 % for RVD and 92.3 ± 2.3 % for RVI. Bumetanide 50 μM inhibited volume recovery, from 92.3 ± 2.3 % (control) to 24.6 ± 8.8 % and reduced the rate of RVI from 0.070 ± 0.020 intensity/min (control) to 0.010 ± 0.005 intensity/min. 50 μM DIDS reduced volume recovery to 42.93 ± 7.7 % and rate of RVI, to 0.03 ± 0.01 intensity/min. Conclusions: these results suggest that bumetanide- and DIDS-sensitive mechanisms are involved in the RVI of HL-1 cells, which points to the involvement of the Na+/K+/2Cl- cotransporter and Cl-/bicarbonate exchanger in RVI, respectively. © 2014 S. Karger AG, Basel. |
| title |
Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes |
| title_short |
Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes |
| title_full |
Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes |
| title_fullStr |
Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes |
| title_full_unstemmed |
Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes |
| title_sort |
regulatory volume increase and regulatory volume decrease responses in hl-1 atrial myocytes |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v33_n6_p1745_Cacace http://hdl.handle.net/20.500.12110/paper_10158987_v33_n6_p1745_Cacace |
| _version_ |
1768544513078329344 |