Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line

Background/ Aims: Since the reversible phosphorylation of tyrosyl residues is a critical event in cellular signaling pathways activated by erythropoietin (Epo), attention has been focused on protein tyrosine phosphatases (PTPs) and their coordinated action with protein tyrosine kinases. The prototyp...

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Detalles Bibliográficos
Autores principales: Callero, Mariana A., Pérez, Gladys Mabel, Vittori, Daniela Cecilia, Pregi, Nicolás, Nesse, Alcira Beatriz
Publicado: 2007
Materias:
Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v20_n5_p319_Callero
http://hdl.handle.net/20.500.12110/paper_10158987_v20_n5_p319_Callero
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_10158987_v20_n5_p319_Callero
record_format dspace
spelling paper:paper_10158987_v20_n5_p319_Callero2023-06-08T15:59:46Z Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line Callero, Mariana A. Pérez, Gladys Mabel Vittori, Daniela Cecilia Pregi, Nicolás Nesse, Alcira Beatriz Erythropoietin Protein Tyrosine Phosphatase 1B UT-7 cell line 4 nitrophenylphosphatase erythropoietin Janus kinase 2 phosphatidylinositol 3 kinase protein tyrosine phosphatase 1B article controlled study enzyme activity enzyme phosphorylation feedback system human human cell hydrolysis priority journal protein expression protein function real time polymerase chain reaction signal transduction Western blotting 1-Phosphatidylinositol 3-Kinase Cell Line Cell Survival Down-Regulation Erythropoietin Gene Expression Regulation, Enzymologic Janus Kinase 2 Phosphorylation Protein-Tyrosine-Phosphatase Tyrphostins Background/ Aims: Since the reversible phosphorylation of tyrosyl residues is a critical event in cellular signaling pathways activated by erythropoietin (Epo), attention has been focused on protein tyrosine phosphatases (PTPs) and their coordinated action with protein tyrosine kinases. The prototypic member of the PTP family is PTP1B, a widely expressed non-receptor PTP located both in cytosol and intracellular membranes via its hydrophobic C-terminal targeting sequence. PTP1B has been implicated in the regulation of signaling pathways involving tyrosine phosphorylation induced by growth factors, cytokines, and hormones, such as the downregulation of erythropoietin and insulin receptors. However, little is known about which factor modulates the activity of this enzyme. Methods: The effect of Epo on PTP1B expression was studied in the UT-7 Epo-dependent cell line. PTP1B expression was analyzed under different conditions by Real-Time PCR and Western blot, while PTP1B phosphatase activity was determined by a p-nitrophenylphosphate hydrolysis assay. Results: Epo rapidly induced an increased expression of PTP1B which was associated with higher PTP1B tyrosine phosphorylation and phosphatase activity. The action of Epo on PTP1B induction involved Janus Kinase 2 (JAK2) and Phosphatidylinositol-3 kinase (PI3K). Conclusion: The results allow us to suggest for the first time that, besides modulating Epo/Epo receptor signaling, PTP1B undergoes feedback regulation by Epo. Copyright © 2007 S. Karger AG. Fil:Callero, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v20_n5_p319_Callero http://hdl.handle.net/20.500.12110/paper_10158987_v20_n5_p319_Callero
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Erythropoietin
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
spellingShingle Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Erythropoietin
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
Callero, Mariana A.
Pérez, Gladys Mabel
Vittori, Daniela Cecilia
Pregi, Nicolás
Nesse, Alcira Beatriz
Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
topic_facet Erythropoietin
Protein Tyrosine Phosphatase 1B
UT-7 cell line
4 nitrophenylphosphatase
erythropoietin
Janus kinase 2
phosphatidylinositol 3 kinase
protein tyrosine phosphatase 1B
article
controlled study
enzyme activity
enzyme phosphorylation
feedback system
human
human cell
hydrolysis
priority journal
protein expression
protein function
real time polymerase chain reaction
signal transduction
Western blotting
1-Phosphatidylinositol 3-Kinase
Cell Line
Cell Survival
Down-Regulation
Erythropoietin
Gene Expression Regulation, Enzymologic
Janus Kinase 2
Phosphorylation
Protein-Tyrosine-Phosphatase
Tyrphostins
description Background/ Aims: Since the reversible phosphorylation of tyrosyl residues is a critical event in cellular signaling pathways activated by erythropoietin (Epo), attention has been focused on protein tyrosine phosphatases (PTPs) and their coordinated action with protein tyrosine kinases. The prototypic member of the PTP family is PTP1B, a widely expressed non-receptor PTP located both in cytosol and intracellular membranes via its hydrophobic C-terminal targeting sequence. PTP1B has been implicated in the regulation of signaling pathways involving tyrosine phosphorylation induced by growth factors, cytokines, and hormones, such as the downregulation of erythropoietin and insulin receptors. However, little is known about which factor modulates the activity of this enzyme. Methods: The effect of Epo on PTP1B expression was studied in the UT-7 Epo-dependent cell line. PTP1B expression was analyzed under different conditions by Real-Time PCR and Western blot, while PTP1B phosphatase activity was determined by a p-nitrophenylphosphate hydrolysis assay. Results: Epo rapidly induced an increased expression of PTP1B which was associated with higher PTP1B tyrosine phosphorylation and phosphatase activity. The action of Epo on PTP1B induction involved Janus Kinase 2 (JAK2) and Phosphatidylinositol-3 kinase (PI3K). Conclusion: The results allow us to suggest for the first time that, besides modulating Epo/Epo receptor signaling, PTP1B undergoes feedback regulation by Epo. Copyright © 2007 S. Karger AG.
author Callero, Mariana A.
Pérez, Gladys Mabel
Vittori, Daniela Cecilia
Pregi, Nicolás
Nesse, Alcira Beatriz
author_facet Callero, Mariana A.
Pérez, Gladys Mabel
Vittori, Daniela Cecilia
Pregi, Nicolás
Nesse, Alcira Beatriz
author_sort Callero, Mariana A.
title Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_short Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_full Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_fullStr Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_full_unstemmed Modulation of protein tyrosine phosphatase 1B by erythropoietin in UT-7 cell line
title_sort modulation of protein tyrosine phosphatase 1b by erythropoietin in ut-7 cell line
publishDate 2007
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v20_n5_p319_Callero
http://hdl.handle.net/20.500.12110/paper_10158987_v20_n5_p319_Callero
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