Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations
Transgenic Nicotiana tabacum plants expressing the TGBp1 movement protein of potato virus X (PVX) were studied to investigate the effects caused by this protein on plant physiology and development. TGBp1 caused consistent reductions of size and weight in different organs of these plants; however sho...
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2004
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paper:paper_09819428_v42_n9_p731_Kobayashi2023-06-08T15:59:14Z Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations Kobayashi, Ken Ares, Vera Ximena Rivero Pérez, María Mercedes Mentaberry, Alejandro Néstor Chloroplast Movement protein Physiology Potexvirus Tobacco chlorophyll primer DNA virus protein article gene expression regulation genetics growth, development and aging metabolism photosynthesis physiology polymerase chain reaction Potexvirus tobacco transgenic plant virology Chlorophyll DNA Primers Gene Expression Regulation, Viral Photosynthesis Plants, Genetically Modified Polymerase Chain Reaction Potexvirus Tobacco Viral Proteins Nicotiana Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potexvirus Solanum tuberosum Transgenic Nicotiana tabacum plants expressing the TGBp1 movement protein of potato virus X (PVX) were studied to investigate the effects caused by this protein on plant physiology and development. TGBp1 caused consistent reductions of size and weight in different organs of these plants; however shoot-to-root ratios were similar to those of control plants. Transgenic seedlings showed smaller root meristems and calli derived from TGBp1 leaves grew at a slower rate through successive subcultures. Microscopic observations of TGBp1 plants revealed flattened chloroplasts containing plastoglobuli-like bodies. Further analyses showed a considerable reduction in photosynthetic rate, lower starch levels in leaves and roots, higher nitrate accumulation in leaves and induction of pathogenesis-related (PR) protein genes. Since these changes were not observed when other PVX sequences were expressed in tobacco, we postulate that TGBp1 is an important symptom contributor in PVX infections. © 2004 Elsevier SAS. All rights reserved. Fil:Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ares, X. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rivero, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mentaberry, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v42_n9_p731_Kobayashi http://hdl.handle.net/20.500.12110/paper_09819428_v42_n9_p731_Kobayashi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chloroplast Movement protein Physiology Potexvirus Tobacco chlorophyll primer DNA virus protein article gene expression regulation genetics growth, development and aging metabolism photosynthesis physiology polymerase chain reaction Potexvirus tobacco transgenic plant virology Chlorophyll DNA Primers Gene Expression Regulation, Viral Photosynthesis Plants, Genetically Modified Polymerase Chain Reaction Potexvirus Tobacco Viral Proteins Nicotiana Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potexvirus Solanum tuberosum |
| spellingShingle |
Chloroplast Movement protein Physiology Potexvirus Tobacco chlorophyll primer DNA virus protein article gene expression regulation genetics growth, development and aging metabolism photosynthesis physiology polymerase chain reaction Potexvirus tobacco transgenic plant virology Chlorophyll DNA Primers Gene Expression Regulation, Viral Photosynthesis Plants, Genetically Modified Polymerase Chain Reaction Potexvirus Tobacco Viral Proteins Nicotiana Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potexvirus Solanum tuberosum Kobayashi, Ken Ares, Vera Ximena Rivero Pérez, María Mercedes Mentaberry, Alejandro Néstor Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
| topic_facet |
Chloroplast Movement protein Physiology Potexvirus Tobacco chlorophyll primer DNA virus protein article gene expression regulation genetics growth, development and aging metabolism photosynthesis physiology polymerase chain reaction Potexvirus tobacco transgenic plant virology Chlorophyll DNA Primers Gene Expression Regulation, Viral Photosynthesis Plants, Genetically Modified Polymerase Chain Reaction Potexvirus Tobacco Viral Proteins Nicotiana Nicotiana obtusifolia Nicotiana tabacum Potato virus X Potexvirus Solanum tuberosum |
| description |
Transgenic Nicotiana tabacum plants expressing the TGBp1 movement protein of potato virus X (PVX) were studied to investigate the effects caused by this protein on plant physiology and development. TGBp1 caused consistent reductions of size and weight in different organs of these plants; however shoot-to-root ratios were similar to those of control plants. Transgenic seedlings showed smaller root meristems and calli derived from TGBp1 leaves grew at a slower rate through successive subcultures. Microscopic observations of TGBp1 plants revealed flattened chloroplasts containing plastoglobuli-like bodies. Further analyses showed a considerable reduction in photosynthetic rate, lower starch levels in leaves and roots, higher nitrate accumulation in leaves and induction of pathogenesis-related (PR) protein genes. Since these changes were not observed when other PVX sequences were expressed in tobacco, we postulate that TGBp1 is an important symptom contributor in PVX infections. © 2004 Elsevier SAS. All rights reserved. |
| author |
Kobayashi, Ken Ares, Vera Ximena Rivero Pérez, María Mercedes Mentaberry, Alejandro Néstor |
| author_facet |
Kobayashi, Ken Ares, Vera Ximena Rivero Pérez, María Mercedes Mentaberry, Alejandro Néstor |
| author_sort |
Kobayashi, Ken |
| title |
Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
| title_short |
Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
| title_full |
Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
| title_fullStr |
Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
| title_full_unstemmed |
Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
| title_sort |
over-expression of potato virus x tgbp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v42_n9_p731_Kobayashi http://hdl.handle.net/20.500.12110/paper_09819428_v42_n9_p731_Kobayashi |
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1768542898430672896 |